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Neuroprotective effects of Dendrobium alkaloids on rat cortical neurons injured by oxygen-glucose deprivation and reperfusion.
Phytomedicine. 2010 Feb; 17(2):108-15.P

Abstract

In this study we investigated the protective effects of alkaloids from Dendrobium spez. on cortical neurons injured by oxygen-glucose deprivation/reperfusion (OGD/RP) in vitro. Rat primary cultured cerebral cortical neurons were investigated at different time points of OGD/RP. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine cell viability. The concentration of intracellular free calcium [Ca(2+)](i) and mitochondrial membrane potential (MMP) were determined to evaluate the degree of neuron damage. Morphologic changes of neurons following OGD/RP were examined by electron microscope. To evaluate neuron apoptosis, flow cytometry was performed and the expressions of caspase-3 and caspase-12 mRNA were examined by real-time quantitative PCR during OGD 2h/RP 12h. Treatment with Dendrobium alkaloids (0.025 approximately 2.5mg/l) significantly attenuated neuronal damage, with evidence of increased cell viability, decreased cell apoptosis, and decreased cell morphologic impairment. Furthermore, Dendrobium alkaloids inhibited [Ca(2+)](i) elevation, increased MMP and decreased the expressions of caspase-3 and caspase-12 in a concentration-dependent manner at OGD 2h/RP 12h. Dendrobium alkaloids have significantly protective effects on OGD/RP-induced neuronal damages in rat primary neuron cultures. The protection against OGD/RP-induced apoptosis appears to be mediated through blocking the decrease in MMP and increase in [Ca(2+)](i), as well as by down-regulating mRNA expression of caspase-3 and caspase-12.

Authors+Show Affiliations

Department of Pharmacology, Zunyi Medical College, 201 Dalian Road, Zunyi 563000, China. wangqianlily2006@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19577451

Citation

Wang, Q, et al. "Neuroprotective Effects of Dendrobium Alkaloids On Rat Cortical Neurons Injured By Oxygen-glucose Deprivation and Reperfusion." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 17, no. 2, 2010, pp. 108-15.
Wang Q, Gong Q, Wu Q, et al. Neuroprotective effects of Dendrobium alkaloids on rat cortical neurons injured by oxygen-glucose deprivation and reperfusion. Phytomedicine. 2010;17(2):108-15.
Wang, Q., Gong, Q., Wu, Q., & Shi, J. (2010). Neuroprotective effects of Dendrobium alkaloids on rat cortical neurons injured by oxygen-glucose deprivation and reperfusion. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 17(2), 108-15. https://doi.org/10.1016/j.phymed.2009.05.010
Wang Q, et al. Neuroprotective Effects of Dendrobium Alkaloids On Rat Cortical Neurons Injured By Oxygen-glucose Deprivation and Reperfusion. Phytomedicine. 2010;17(2):108-15. PubMed PMID: 19577451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effects of Dendrobium alkaloids on rat cortical neurons injured by oxygen-glucose deprivation and reperfusion. AU - Wang,Q, AU - Gong,Q, AU - Wu,Q, AU - Shi,J, Y1 - 2009/07/03/ PY - 2008/03/06/received PY - 2009/04/08/revised PY - 2009/05/19/accepted PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2010/6/9/medline SP - 108 EP - 15 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 17 IS - 2 N2 - In this study we investigated the protective effects of alkaloids from Dendrobium spez. on cortical neurons injured by oxygen-glucose deprivation/reperfusion (OGD/RP) in vitro. Rat primary cultured cerebral cortical neurons were investigated at different time points of OGD/RP. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine cell viability. The concentration of intracellular free calcium [Ca(2+)](i) and mitochondrial membrane potential (MMP) were determined to evaluate the degree of neuron damage. Morphologic changes of neurons following OGD/RP were examined by electron microscope. To evaluate neuron apoptosis, flow cytometry was performed and the expressions of caspase-3 and caspase-12 mRNA were examined by real-time quantitative PCR during OGD 2h/RP 12h. Treatment with Dendrobium alkaloids (0.025 approximately 2.5mg/l) significantly attenuated neuronal damage, with evidence of increased cell viability, decreased cell apoptosis, and decreased cell morphologic impairment. Furthermore, Dendrobium alkaloids inhibited [Ca(2+)](i) elevation, increased MMP and decreased the expressions of caspase-3 and caspase-12 in a concentration-dependent manner at OGD 2h/RP 12h. Dendrobium alkaloids have significantly protective effects on OGD/RP-induced neuronal damages in rat primary neuron cultures. The protection against OGD/RP-induced apoptosis appears to be mediated through blocking the decrease in MMP and increase in [Ca(2+)](i), as well as by down-regulating mRNA expression of caspase-3 and caspase-12. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/19577451/Neuroprotective_effects_of_Dendrobium_alkaloids_on_rat_cortical_neurons_injured_by_oxygen_glucose_deprivation_and_reperfusion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(09)00134-2 DB - PRIME DP - Unbound Medicine ER -