TY - JOUR T1 - Genetic analysis of avian influenza A viruses isolated from domestic waterfowl in live-bird markets of Hanoi, Vietnam, preceding fatal H5N1 human infections in 2004. AU - Jadhao,Samadhan J, AU - Nguyen,Doan C, AU - Uyeki,Timothy M, AU - Shaw,Michael, AU - Maines,Taronna, AU - Rowe,Thomas, AU - Smith,Catherine, AU - Huynh,Lien P T, AU - Nghiem,Ha K, AU - Nguyen,Diep H T, AU - Nguyen,Hang K L, AU - Nguyen,Hanh H T, AU - Hoang,Long T, AU - Nguyen,Tung, AU - Phuong,Lien S, AU - Klimov,Alexander, AU - Tumpey,Terrence M, AU - Cox,Nancy J, AU - Donis,Ruben O, AU - Matsuoka,Yumiko, AU - Katz,Jacqueline M, Y1 - 2009/07/04/ PY - 2009/01/19/received PY - 2009/06/09/accepted PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2009/10/3/medline SP - 1249 EP - 61 JF - Archives of virology JO - Arch Virol VL - 154 IS - 8 N2 - The first known cases of human infection with highly pathogenic avian influenza (HPAI) H5N1 viruses in Vietnam occurred in late 2003. However, HPAI H5N1 and low-pathogenic avian influenza (LPAI) H5N2 and H9N3 viruses were isolated from domestic waterfowl during live-bird market (LBM) surveillance in Vietnam in 2001 and 2003. To understand the possible role of these early viruses in the genesis of H5N1 strains infecting people, we performed sequencing and molecular characterization. Phylogenetic analysis revealed that the hemagglutinin (HA) genes of two geese HPAI H5N1 strains belonged to clade 3, and their surface glycoprotein and replication complex genes were most closely related (98.5-99.7% homologous) to A/duck/Guangxi/22/01 (H5N1) virus, detected contemporarily in southern China, whilst the M and NS genes were derived from an A/duck/Hong Kong/2986.1/00 (H5N1)-like virus. The H5 HA gene of the duck HPAI H5N1 strain belonged to clade 5 and acquired a gene constellation from A/quail/Shantou/3846/02 (H5N1), A/teal/China/2978.1/02 (H5N1) and A/partridge/Shantou/2286/03 (H5N1)-like viruses. The phylogenetic analysis further indicated that all eight gene segments of goose and duck HPAI H5N1 and LPAI H5N2 viruses were distinct from those of H5N1 clade-1 viruses known to have caused fatal human infections in Vietnam since late 2003. The duck H9N3 isolates derived genes from aquatic-bird influenza viruses, and their H9 HA belonged to the Korean lineage. The PB2 gene of A/duck/Vietnam/340/01 (H9N3) virus had lysine at position 627. Based on the molecular characterization of specific amino acid residues in the surface and relevant internal protein-coding genes, the Vietnamese H5N1 and H9N3 virus isolates indicated specificity to avian cell surface receptor and susceptibility for currently licensed anti-influenza A virus chemotherapeutics. Our findings suggest that the H5N1 and H5N2 viruses that circulated among geese and ducks in LBMs in Hanoi, Vietnam, during 2001 and 2003 were not the immediate ancestors of the clade-1 viruses associated with fatal human infections in Vietnam. The clade-1 HPAI H5N1 viruses were independently introduced into Vietnam. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/19578928/Genetic_analysis_of_avian_influenza_A_viruses_isolated_from_domestic_waterfowl_in_live_bird_markets_of_Hanoi_Vietnam_preceding_fatal_H5N1_human_infections_in_2004_ L2 - https://dx.doi.org/10.1007/s00705-009-0429-2 DB - PRIME DP - Unbound Medicine ER -