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Clustering analysis of Salmonella enterica serovar Typhi isolates in Korea by PFGE, ribotying, and phage typing.
Foodborne Pathog Dis. 2009 Jul-Aug; 6(6):733-8.FP

Abstract

Salmonella enterica serovar Typhi is a Gram-negative bacterium causing the acute febrile disease typhoid fever. In Korea from 2004 to 2006, a total of 51 Salmonella Typhi isolates were identified in stool and blood from healthy carriers and patients with or without overseas travel histories. In this study, antibiogram, pulsed-field gel electrophoresis (PFGE), and automated ribotyping were performed as molecular epidemiological methods with phage typing as a classical subtyping tool of the isolates. Only two isolates were multidrug resistant and 82.3% of the isolates were susceptible to 16 antimicrobial agents tested. When the dendrogram was created based on the PFGE results, the subtypes could be clustered into five groups by 80% similarity criterion. The PFGE patterns of 31 isolates (60.8%) belonged to Cluster 3, the predominant cluster in the study. Three overseas travel-associated cases were differentiated into Cluster 4 of which three isolates were nalidixic acid or multidrug resistant. Major phage type and ribotype were A and PvuII-436-8-S-6, respectively. This study also showed the prevalence of PFGE Cluster 3 in Korea by clustering analysis and the link between some typhoid cases and travel to Cambodia, India, or Indonesia.

Authors+Show Affiliations

Center for Infectious Diseases, Korea National Institute of Health, Seoul, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19580446

Citation

Kim, Shukho, et al. "Clustering Analysis of Salmonella Enterica Serovar Typhi Isolates in Korea By PFGE, Ribotying, and Phage Typing." Foodborne Pathogens and Disease, vol. 6, no. 6, 2009, pp. 733-8.
Kim S, Kim SH, Park JH, et al. Clustering analysis of Salmonella enterica serovar Typhi isolates in Korea by PFGE, ribotying, and phage typing. Foodborne Pathog Dis. 2009;6(6):733-8.
Kim, S., Kim, S. H., Park, J. H., Lee, K. S., Park, M. S., & Lee, B. K. (2009). Clustering analysis of Salmonella enterica serovar Typhi isolates in Korea by PFGE, ribotying, and phage typing. Foodborne Pathogens and Disease, 6(6), 733-8. https://doi.org/10.1089/fpd.2008.0212
Kim S, et al. Clustering Analysis of Salmonella Enterica Serovar Typhi Isolates in Korea By PFGE, Ribotying, and Phage Typing. Foodborne Pathog Dis. 2009 Jul-Aug;6(6):733-8. PubMed PMID: 19580446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clustering analysis of Salmonella enterica serovar Typhi isolates in Korea by PFGE, ribotying, and phage typing. AU - Kim,Shukho, AU - Kim,Sung-Hun, AU - Park,Jeong-Hyun, AU - Lee,Kyung-Shin, AU - Park,Mi-Sun, AU - Lee,Bok Kwon, PY - 2009/7/8/entrez PY - 2009/7/8/pubmed PY - 2009/9/25/medline SP - 733 EP - 8 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 6 IS - 6 N2 - Salmonella enterica serovar Typhi is a Gram-negative bacterium causing the acute febrile disease typhoid fever. In Korea from 2004 to 2006, a total of 51 Salmonella Typhi isolates were identified in stool and blood from healthy carriers and patients with or without overseas travel histories. In this study, antibiogram, pulsed-field gel electrophoresis (PFGE), and automated ribotyping were performed as molecular epidemiological methods with phage typing as a classical subtyping tool of the isolates. Only two isolates were multidrug resistant and 82.3% of the isolates were susceptible to 16 antimicrobial agents tested. When the dendrogram was created based on the PFGE results, the subtypes could be clustered into five groups by 80% similarity criterion. The PFGE patterns of 31 isolates (60.8%) belonged to Cluster 3, the predominant cluster in the study. Three overseas travel-associated cases were differentiated into Cluster 4 of which three isolates were nalidixic acid or multidrug resistant. Major phage type and ribotype were A and PvuII-436-8-S-6, respectively. This study also showed the prevalence of PFGE Cluster 3 in Korea by clustering analysis and the link between some typhoid cases and travel to Cambodia, India, or Indonesia. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/19580446/Clustering_analysis_of_Salmonella_enterica_serovar_Typhi_isolates_in_Korea_by_PFGE_ribotying_and_phage_typing_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2008.0212?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -