Tags

Type your tag names separated by a space and hit enter

Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution.
BMC Microbiol. 2009 Jul 07; 9:132.BM

Abstract

BACKGROUND

Extraintestinal pathogenic Escherichia coli (ExPEC) strains of serotype O1:K1:H7/NM are frequently implicated in neonatal meningitis, urinary tract infections and septicemia in humans. They are also commonly isolated from colibacillosis in poultry. Studies to determine the similarities of ExPEC from different origins have indicated that avian strains potentially have zoonotic properties.

RESULTS

A total of 59 ExPEC O1:K1:H7/NM isolates (21 from avian colibacillosis, 15 from human meningitis, and 23 from human urinary tract infection and septicemia) originated from four countries were characterized by phylogenetic PCR grouping, Multilocus Sequence Typing (MLST), Pulsed Field Gel Electrophoresis (PFGE) and genotyping based on several genes known for their association with ExPEC or avian pathogenic Escherichia coli (APEC) virulence.APEC and human ExPEC isolates differed significantly in their assignments to phylogenetic groups, being phylogroup B2 more prevalent among APEC than among human ExPEC (95% vs. 53%, P = 0.001), whereas phylogroup D was almost exclusively associated with human ExPEC (47% vs. 5%, P = 0.0000). Seven virulence genes showed significant differences, being fimAvMT78 and sat genes linked to human isolates, while papGII, tsh, iron, cvaC and iss were significantly associated to APEC. By MLST, 39 of 40 ExPEC belonging to phylogroup B2, and 17 of 19 belonging to phylogroup D exhibited the Sequence Types (STs) ST95 and ST59, respectively. Additionally, two novel STs (ST1013 and ST1006) were established. Considering strains sharing the same ST, phylogenetic group, virulence genotype and PFGE cluster to belong to the same subclone, five subclones were detected; one of those grouped six strains of human and animal origin from two countries.

CONCLUSION

Present results reveal that the clonal group B2 O1:K1:H7/NM ST95, detected in strains of animal and human origin, recovered from different dates and geographic sources, provides evidence that some APEC isolates may act as potential pathogens for humans and, consequently, poultry as a foodborne source, suggesting no host specificity for this type of isolates. A novel and important finding has been the detection of the clonal group D O1:K1:H7/NM ST59 almost exclusively in humans, carrying pathogenic genes linked to the phylogenetic group D. This finding would suggest D O1:K1:H7/NM ST59 as a host specific pathotype for humans.

Authors+Show Affiliations

Laboratorio de Referencia de E, coli, Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain. azucena.mora@usc.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19583828

Citation

Mora, Azucena, et al. "Extraintestinal Pathogenic Escherichia Coli O1:K1:H7/NM From Human and Avian Origin: Detection of Clonal Groups B2 ST95 and D ST59 With Different Host Distribution." BMC Microbiology, vol. 9, 2009, p. 132.
Mora A, López C, Dabhi G, et al. Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution. BMC Microbiol. 2009;9:132.
Mora, A., López, C., Dabhi, G., Blanco, M., Blanco, J. E., Alonso, M. P., Herrera, A., Mamani, R., Bonacorsi, S., Moulin-Schouleur, M., & Blanco, J. (2009). Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution. BMC Microbiology, 9, 132. https://doi.org/10.1186/1471-2180-9-132
Mora A, et al. Extraintestinal Pathogenic Escherichia Coli O1:K1:H7/NM From Human and Avian Origin: Detection of Clonal Groups B2 ST95 and D ST59 With Different Host Distribution. BMC Microbiol. 2009 Jul 7;9:132. PubMed PMID: 19583828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution. AU - Mora,Azucena, AU - López,Cecilia, AU - Dabhi,Ghizlane, AU - Blanco,Miguel, AU - Blanco,Jesús E, AU - Alonso,María Pilar, AU - Herrera,Alexandra, AU - Mamani,Rosalía, AU - Bonacorsi,Stéphane, AU - Moulin-Schouleur,Maryvonne, AU - Blanco,Jorge, Y1 - 2009/07/07/ PY - 2008/12/30/received PY - 2009/07/07/accepted PY - 2009/7/9/entrez PY - 2009/7/9/pubmed PY - 2009/8/14/medline SP - 132 EP - 132 JF - BMC microbiology JO - BMC Microbiol VL - 9 N2 - BACKGROUND: Extraintestinal pathogenic Escherichia coli (ExPEC) strains of serotype O1:K1:H7/NM are frequently implicated in neonatal meningitis, urinary tract infections and septicemia in humans. They are also commonly isolated from colibacillosis in poultry. Studies to determine the similarities of ExPEC from different origins have indicated that avian strains potentially have zoonotic properties. RESULTS: A total of 59 ExPEC O1:K1:H7/NM isolates (21 from avian colibacillosis, 15 from human meningitis, and 23 from human urinary tract infection and septicemia) originated from four countries were characterized by phylogenetic PCR grouping, Multilocus Sequence Typing (MLST), Pulsed Field Gel Electrophoresis (PFGE) and genotyping based on several genes known for their association with ExPEC or avian pathogenic Escherichia coli (APEC) virulence.APEC and human ExPEC isolates differed significantly in their assignments to phylogenetic groups, being phylogroup B2 more prevalent among APEC than among human ExPEC (95% vs. 53%, P = 0.001), whereas phylogroup D was almost exclusively associated with human ExPEC (47% vs. 5%, P = 0.0000). Seven virulence genes showed significant differences, being fimAvMT78 and sat genes linked to human isolates, while papGII, tsh, iron, cvaC and iss were significantly associated to APEC. By MLST, 39 of 40 ExPEC belonging to phylogroup B2, and 17 of 19 belonging to phylogroup D exhibited the Sequence Types (STs) ST95 and ST59, respectively. Additionally, two novel STs (ST1013 and ST1006) were established. Considering strains sharing the same ST, phylogenetic group, virulence genotype and PFGE cluster to belong to the same subclone, five subclones were detected; one of those grouped six strains of human and animal origin from two countries. CONCLUSION: Present results reveal that the clonal group B2 O1:K1:H7/NM ST95, detected in strains of animal and human origin, recovered from different dates and geographic sources, provides evidence that some APEC isolates may act as potential pathogens for humans and, consequently, poultry as a foodborne source, suggesting no host specificity for this type of isolates. A novel and important finding has been the detection of the clonal group D O1:K1:H7/NM ST59 almost exclusively in humans, carrying pathogenic genes linked to the phylogenetic group D. This finding would suggest D O1:K1:H7/NM ST59 as a host specific pathotype for humans. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/19583828/Extraintestinal_pathogenic_Escherichia_coli_O1:K1:H7/NM_from_human_and_avian_origin:_detection_of_clonal_groups_B2_ST95_and_D_ST59_with_different_host_distribution_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-9-132 DB - PRIME DP - Unbound Medicine ER -