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Towards the mechanism and comparative effect of diphenyl diselenide, diphenyl ditelluride and ebselen under various pathophysiological conditions in rat's kidney preparation.
Chem Biol Interact. 2009 Nov 10; 182(1):52-8.CB

Abstract

As an extension of our previous work we not only evaluated the relationship between acidosis and lipid peroxidation in rat's kidney homogenate, but also determined for the first time the potential anti-oxidant activity of diphenyl diselenide, diphenyl ditelluride and ebselen at a range of pH values (7.4-5.4). Because of the pH dependency of iron redox cycling, pH and iron need to be well controlled and for the reason we tested a number of pH values (from 7.4 to 5.4) to get a closer idea about the role of iron under various pathological conditions. Acidosis increased rate of lipid peroxidation in the absence Fe (II) in kidney homogenates especially at pH 5.4. This higher extent of lipid peroxidation can be explained by; the mobilized iron which may come from reserves where it is weakly bound. Addition of iron (Fe) chelator desferoxamine (DFO) to reaction medium completely inhibited the peroxidation processes at all studied pH values including acidic values (5.8-5.4). In the presence of Fe (II) acidosis also enhanced detrimental effect of Fe (II) especially at pH (6.4-5.4). Diphenyl diselenide significantly protected lipid peroxidation at all studied pH values, while ebselen offered only a small statistically non-significant protection. The highest anti-oxidant potency was observed for diphenyl ditelluride. These differences in potencies were explained by the mode of action of these compounds using their catalytic anti-oxidant cycles. However, changing the pH of the reaction medium did not alter the anti-oxidant activity of the tested compounds. This study provides evidence for acidosis catalyzed oxidative stress in kidney homogenate and for the first time anti-oxidant potential of diphenyl diselenide and diphenyl ditelluride not only at physiological pH but also at a range of acidic values.

Authors+Show Affiliations

Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, RS, Brazil. waseem anw@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19589335

Citation

Hassan, Waseem, et al. "Towards the Mechanism and Comparative Effect of Diphenyl Diselenide, Diphenyl Ditelluride and Ebselen Under Various Pathophysiological Conditions in Rat's Kidney Preparation." Chemico-biological Interactions, vol. 182, no. 1, 2009, pp. 52-8.
Hassan W, Ibrahim M, Rocha JB. Towards the mechanism and comparative effect of diphenyl diselenide, diphenyl ditelluride and ebselen under various pathophysiological conditions in rat's kidney preparation. Chem Biol Interact. 2009;182(1):52-8.
Hassan, W., Ibrahim, M., & Rocha, J. B. (2009). Towards the mechanism and comparative effect of diphenyl diselenide, diphenyl ditelluride and ebselen under various pathophysiological conditions in rat's kidney preparation. Chemico-biological Interactions, 182(1), 52-8. https://doi.org/10.1016/j.cbi.2009.06.023
Hassan W, Ibrahim M, Rocha JB. Towards the Mechanism and Comparative Effect of Diphenyl Diselenide, Diphenyl Ditelluride and Ebselen Under Various Pathophysiological Conditions in Rat's Kidney Preparation. Chem Biol Interact. 2009 Nov 10;182(1):52-8. PubMed PMID: 19589335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Towards the mechanism and comparative effect of diphenyl diselenide, diphenyl ditelluride and ebselen under various pathophysiological conditions in rat's kidney preparation. AU - Hassan,Waseem, AU - Ibrahim,Mohammad, AU - Rocha,Joao Batista Teixeira, Y1 - 2009/07/07/ PY - 2009/05/21/received PY - 2009/06/23/revised PY - 2009/06/29/accepted PY - 2009/7/11/entrez PY - 2009/7/11/pubmed PY - 2009/10/30/medline SP - 52 EP - 8 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 182 IS - 1 N2 - As an extension of our previous work we not only evaluated the relationship between acidosis and lipid peroxidation in rat's kidney homogenate, but also determined for the first time the potential anti-oxidant activity of diphenyl diselenide, diphenyl ditelluride and ebselen at a range of pH values (7.4-5.4). Because of the pH dependency of iron redox cycling, pH and iron need to be well controlled and for the reason we tested a number of pH values (from 7.4 to 5.4) to get a closer idea about the role of iron under various pathological conditions. Acidosis increased rate of lipid peroxidation in the absence Fe (II) in kidney homogenates especially at pH 5.4. This higher extent of lipid peroxidation can be explained by; the mobilized iron which may come from reserves where it is weakly bound. Addition of iron (Fe) chelator desferoxamine (DFO) to reaction medium completely inhibited the peroxidation processes at all studied pH values including acidic values (5.8-5.4). In the presence of Fe (II) acidosis also enhanced detrimental effect of Fe (II) especially at pH (6.4-5.4). Diphenyl diselenide significantly protected lipid peroxidation at all studied pH values, while ebselen offered only a small statistically non-significant protection. The highest anti-oxidant potency was observed for diphenyl ditelluride. These differences in potencies were explained by the mode of action of these compounds using their catalytic anti-oxidant cycles. However, changing the pH of the reaction medium did not alter the anti-oxidant activity of the tested compounds. This study provides evidence for acidosis catalyzed oxidative stress in kidney homogenate and for the first time anti-oxidant potential of diphenyl diselenide and diphenyl ditelluride not only at physiological pH but also at a range of acidic values. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/19589335/Towards_the_mechanism_and_comparative_effect_of_diphenyl_diselenide_diphenyl_ditelluride_and_ebselen_under_various_pathophysiological_conditions_in_rat's_kidney_preparation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(09)00270-1 DB - PRIME DP - Unbound Medicine ER -