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Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function.
Apoptosis. 2009 Sep; 14(9):1050-60.A

Abstract

Insulin has been shown to possess significant anti-apoptotic effect in myocardial ischemia/reperfusion (MI/R). However, the contribution by this protection of insulin to the prolonged cardiac function in rats subjected to ischemia remains unclear. The present study attempted to test whether early insulin treatment influences adverse prolonged post-ischemic cardiac structural and functional changes. Adult male rats were subjected to left anterior descending coronary artery occlusion and were randomized to receive one of the following treatments: saline (4 ml/kg/h i.v. injection beginning 10 min before the ischemia and continuing for 2 h), insulin (60 U/l, i.v. injection following the same routine, and hypodermic injection of insulin (0.5 U/ml, 1 ml/kg/d) for 3 days after the ischemia surgery) or insulin plus wortmannin (15 mug/kg i.v. injection 15 min before each insulin administration). Treatment with insulin significantly reduced infarct size, decreased plasma creatine kinase and lactate dehydrogenase activities, decreased apoptosis index and caspase-3 activity (all P < 0.01 vs. saline), and improved cardiac function 24 h after ischemia. Importantly, at the end of 4 weeks after the ischemia surgery, MI rats receiving insulin treatment showed smaller left ventricle (LV) cavity and thicker systolic interventricular septum, and increased cardiac ejection fraction and LV fractional shortening (all P < 0.05 vs. saline). Inhibition of insulin signaling with wortmannin not only blocked insulin's anti-apoptotic effect, but also almost completely abolished effects of insulin on cardiac structure and function. These data indicate that inhibition of apoptosis by early insulin treatment alleviates chronic adverse changes in post-ischemic cardiac structure and function.

Authors+Show Affiliations

Department of Physiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19590963

Citation

Xing, Wenjuan, et al. "Insulin Inhibits Myocardial Ischemia-induced Apoptosis and Alleviates Chronic Adverse Changes in Post-ischemic Cardiac Structure and Function." Apoptosis : an International Journal On Programmed Cell Death, vol. 14, no. 9, 2009, pp. 1050-60.
Xing W, Yan W, Fu F, et al. Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function. Apoptosis. 2009;14(9):1050-60.
Xing, W., Yan, W., Fu, F., Jin, Y., Ji, L., Liu, W., Wang, L., Lv, A., Duan, Y., Zhang, J., Zhang, H., & Gao, F. (2009). Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function. Apoptosis : an International Journal On Programmed Cell Death, 14(9), 1050-60. https://doi.org/10.1007/s10495-009-0378-y
Xing W, et al. Insulin Inhibits Myocardial Ischemia-induced Apoptosis and Alleviates Chronic Adverse Changes in Post-ischemic Cardiac Structure and Function. Apoptosis. 2009;14(9):1050-60. PubMed PMID: 19590963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function. AU - Xing,Wenjuan, AU - Yan,Wenjun, AU - Fu,Feng, AU - Jin,Yulan, AU - Ji,Lele, AU - Liu,Wenchong, AU - Wang,Li, AU - Lv,Anlin, AU - Duan,Yunyan, AU - Zhang,Jun, AU - Zhang,Haifeng, AU - Gao,Feng, PY - 2009/7/11/entrez PY - 2009/7/11/pubmed PY - 2009/10/22/medline SP - 1050 EP - 60 JF - Apoptosis : an international journal on programmed cell death JO - Apoptosis VL - 14 IS - 9 N2 - Insulin has been shown to possess significant anti-apoptotic effect in myocardial ischemia/reperfusion (MI/R). However, the contribution by this protection of insulin to the prolonged cardiac function in rats subjected to ischemia remains unclear. The present study attempted to test whether early insulin treatment influences adverse prolonged post-ischemic cardiac structural and functional changes. Adult male rats were subjected to left anterior descending coronary artery occlusion and were randomized to receive one of the following treatments: saline (4 ml/kg/h i.v. injection beginning 10 min before the ischemia and continuing for 2 h), insulin (60 U/l, i.v. injection following the same routine, and hypodermic injection of insulin (0.5 U/ml, 1 ml/kg/d) for 3 days after the ischemia surgery) or insulin plus wortmannin (15 mug/kg i.v. injection 15 min before each insulin administration). Treatment with insulin significantly reduced infarct size, decreased plasma creatine kinase and lactate dehydrogenase activities, decreased apoptosis index and caspase-3 activity (all P < 0.01 vs. saline), and improved cardiac function 24 h after ischemia. Importantly, at the end of 4 weeks after the ischemia surgery, MI rats receiving insulin treatment showed smaller left ventricle (LV) cavity and thicker systolic interventricular septum, and increased cardiac ejection fraction and LV fractional shortening (all P < 0.05 vs. saline). Inhibition of insulin signaling with wortmannin not only blocked insulin's anti-apoptotic effect, but also almost completely abolished effects of insulin on cardiac structure and function. These data indicate that inhibition of apoptosis by early insulin treatment alleviates chronic adverse changes in post-ischemic cardiac structure and function. SN - 1573-675X UR - https://www.unboundmedicine.com/medline/citation/19590963/Insulin_inhibits_myocardial_ischemia_induced_apoptosis_and_alleviates_chronic_adverse_changes_in_post_ischemic_cardiac_structure_and_function_ L2 - https://doi.org/10.1007/s10495-009-0378-y DB - PRIME DP - Unbound Medicine ER -