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Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15; 877(24):2487-92.JC

Abstract

Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v). The targeted compounds isolated, collected and purified by HSCCC were analyzed by high performance liquid chromatography (HPLC). A total of 4.6 mg of isoquercitrin, 9.1 mg of hyperoside and 3.0 mg of astragalin with the purity of 95.8%, 97.5% and 98.3%, respectively, were obtained in one-step separation and less than 6 h from 80 mg of crude extract from the leaves of N. nucifera. The chemical structures of all the three compounds were identified by MS, (1)H NMR, (13)C NMR. Astragalin was obtained from N. nucifera for the first time.

Authors+Show Affiliations

State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330047, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19592314

Citation

Deng, Shengguo, et al. "Isolation and Purification of Three Flavonoid Glycosides From the Leaves of Nelumbo Nucifera (Lotus) By High-speed Counter-current Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 24, 2009, pp. 2487-92.
Deng S, Deng Z, Fan Y, et al. Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(24):2487-92.
Deng, S., Deng, Z., Fan, Y., Peng, Y., Li, J., Xiong, D., & Liu, R. (2009). Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(24), 2487-92. https://doi.org/10.1016/j.jchromb.2009.06.026
Deng S, et al. Isolation and Purification of Three Flavonoid Glycosides From the Leaves of Nelumbo Nucifera (Lotus) By High-speed Counter-current Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15;877(24):2487-92. PubMed PMID: 19592314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography. AU - Deng,Shengguo, AU - Deng,Zeyuan, AU - Fan,Yawei, AU - Peng,You, AU - Li,Jing, AU - Xiong,Dongmei, AU - Liu,Rong, Y1 - 2009/06/23/ PY - 2009/02/02/received PY - 2009/06/08/revised PY - 2009/06/12/accepted PY - 2009/7/14/entrez PY - 2009/7/14/pubmed PY - 2009/9/25/medline SP - 2487 EP - 92 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 877 IS - 24 N2 - Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v). The targeted compounds isolated, collected and purified by HSCCC were analyzed by high performance liquid chromatography (HPLC). A total of 4.6 mg of isoquercitrin, 9.1 mg of hyperoside and 3.0 mg of astragalin with the purity of 95.8%, 97.5% and 98.3%, respectively, were obtained in one-step separation and less than 6 h from 80 mg of crude extract from the leaves of N. nucifera. The chemical structures of all the three compounds were identified by MS, (1)H NMR, (13)C NMR. Astragalin was obtained from N. nucifera for the first time. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/19592314/Isolation_and_purification_of_three_flavonoid_glycosides_from_the_leaves_of_Nelumbo_nucifera__Lotus__by_high_speed_counter_current_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00426-7 DB - PRIME DP - Unbound Medicine ER -