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Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling.
J Exp Bot. 2009; 60(11):3221-38.JE

Abstract

In maize (Zea mays), abscisic acid (ABA)-induced H(2)O(2) production activates a 46 kDa mitogen-activated protein kinase (p46MAPK), and the activation of p46MAPK also regulates the production of H(2)O(2). However, the mechanism for the regulation of H(2)O(2) production by MAPK in ABA signalling remains to be elucidated. In this study, four reactive oxygen species (ROS)-producing NADPH oxidase (rboh) genes (ZmrbohA-D) were isolated and characterized in maize leaves. ABA treatment induced a biphasic response (phase I and phase II) in the expression of ZmrbohA-D and the activity of NADPH oxidase. Phase II induced by ABA was blocked by pretreatments with two MAPK kinase (MPKKK) inhibitors and two H(2)O(2) scavengers, but phase I was not affected by these inhibitors or scavengers. Treatment with H(2)O(2) alone also only induced phase II, and the induction was arrested by the MAPKK inhibitors. Furthermore, the ABA-activated p46MAPK was partially purified. Using primers corresponding to the sequences of internal tryptic peptides, the p46MAPK gene was cloned. Analysis of the tryptic peptides and the p46MAPK sequence indicate it is the known ZmMPK5. Treatments with ABA and H(2)O(2) led to a significant increase in the activity of ZmMPK5, although ABA treatment only induced a slight increase in the expression of ZmMPK5. The data indicate that H(2)O(2)-activated ZmMPK5 is involved in the activation of phase II in ABA signalling, but not in phase I. The results suggest that there is a positive feedback loop involving NADPH oxidase, H(2)O(2), and ZmMPK5 in ABA signalling.

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Authors+Show Affiliations

Lin F
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Ding H
No affiliation info available
Wang J
No affiliation info available
Zhang H
No affiliation info available
Zhang A
No affiliation info available
Zhang Y
No affiliation info available
Tan M
No affiliation info available
Dong W
No affiliation info available
Jiang M
No affiliation info available

MeSH

Abscisic AcidAmino Acid SequenceGene Expression Regulation, EnzymologicGene Expression Regulation, PlantHydrogen PeroxideMitogen-Activated Protein KinasesMolecular Sequence DataNADPH OxidasesPlant ProteinsSequence AlignmentSignal TransductionZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19592501

Citation

Lin, Fan, et al. "Positive Feedback Regulation of Maize NADPH Oxidase By Mitogen-activated Protein Kinase Cascade in Abscisic Acid Signalling." Journal of Experimental Botany, vol. 60, no. 11, 2009, pp. 3221-38.
Lin F, Ding H, Wang J, et al. Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling. J Exp Bot. 2009;60(11):3221-38.
Lin, F., Ding, H., Wang, J., Zhang, H., Zhang, A., Zhang, Y., Tan, M., Dong, W., & Jiang, M. (2009). Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling. Journal of Experimental Botany, 60(11), 3221-38. https://doi.org/10.1093/jxb/erp157
Lin F, et al. Positive Feedback Regulation of Maize NADPH Oxidase By Mitogen-activated Protein Kinase Cascade in Abscisic Acid Signalling. J Exp Bot. 2009;60(11):3221-38. PubMed PMID: 19592501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling. AU - Lin,Fan, AU - Ding,Haidong, AU - Wang,Jinxiang, AU - Zhang,Hong, AU - Zhang,Aying, AU - Zhang,Yun, AU - Tan,Mingpu, AU - Dong,Wen, AU - Jiang,Mingyi, Y1 - 2009/07/10/ PY - 2009/7/14/entrez PY - 2009/7/14/pubmed PY - 2009/10/20/medline SP - 3221 EP - 38 JF - Journal of experimental botany JO - J Exp Bot VL - 60 IS - 11 N2 - In maize (Zea mays), abscisic acid (ABA)-induced H(2)O(2) production activates a 46 kDa mitogen-activated protein kinase (p46MAPK), and the activation of p46MAPK also regulates the production of H(2)O(2). However, the mechanism for the regulation of H(2)O(2) production by MAPK in ABA signalling remains to be elucidated. In this study, four reactive oxygen species (ROS)-producing NADPH oxidase (rboh) genes (ZmrbohA-D) were isolated and characterized in maize leaves. ABA treatment induced a biphasic response (phase I and phase II) in the expression of ZmrbohA-D and the activity of NADPH oxidase. Phase II induced by ABA was blocked by pretreatments with two MAPK kinase (MPKKK) inhibitors and two H(2)O(2) scavengers, but phase I was not affected by these inhibitors or scavengers. Treatment with H(2)O(2) alone also only induced phase II, and the induction was arrested by the MAPKK inhibitors. Furthermore, the ABA-activated p46MAPK was partially purified. Using primers corresponding to the sequences of internal tryptic peptides, the p46MAPK gene was cloned. Analysis of the tryptic peptides and the p46MAPK sequence indicate it is the known ZmMPK5. Treatments with ABA and H(2)O(2) led to a significant increase in the activity of ZmMPK5, although ABA treatment only induced a slight increase in the expression of ZmMPK5. The data indicate that H(2)O(2)-activated ZmMPK5 is involved in the activation of phase II in ABA signalling, but not in phase I. The results suggest that there is a positive feedback loop involving NADPH oxidase, H(2)O(2), and ZmMPK5 in ABA signalling. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/19592501/Positive_feedback_regulation_of_maize_NADPH_oxidase_by_mitogen_activated_protein_kinase_cascade_in_abscisic_acid_signalling_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erp157 DB - PRIME DP - Unbound Medicine ER -