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Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients' urine by liquid chromatography-tandem mass spectrometry.
J Pharm Biomed Anal. 2009 Dec 05; 50(5):939-46.JP

Abstract

A novel, selective and sensitive liquid chromatography-tandem mass spectrometry method has been developed and validated for the simultaneous determination of phytoestrogens and their key metabolites in human urine in this study. This method includes internal standard (IS) screening, analytical sample preparation procedure establishment, and linear range investigation. The analytical sample was extracted by liquid-liquid extraction from urine sample. The phytoestrogens and related key metabolites were separated with Agilent Zorbax Eclipse XDB-C18 chromatographic column using methanol and water as mobile phase. The Quattro premier MICROMASS mass spectrometer in negative ion selected reaction monitoring (SRM) mode using electrospray ionization was applied to detect the phytoestrogens and key metabolites. To validate the developed liquid chromatography-tandem mass spectrometry method, the intra- and inter-day precisions, specificity, sensitivity, reproducibility, and sample detective concentration range were evaluated. This is the first reported phytoestrogens analysis and validation study that demonstrates the feasibility of using liquid chromatography-electrospray ionization mass spectrometry to simultaneously analyze ten analytes including both phytoestrogens and their key metabolites in urine samples collected for epidemiological studies in human.

Authors+Show Affiliations

Department of Chinese Medicinal Prescription, China Pharmaceutical University, Nanjing 210038, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19595530

Citation

Yu, Jing, et al. "Simultaneous Determination of Phytoestrogens and Key Metabolites in Breast Cancer Patients' Urine By Liquid Chromatography-tandem Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 50, no. 5, 2009, pp. 939-46.
Yu J, Wu Q, Qiao S, et al. Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients' urine by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2009;50(5):939-46.
Yu, J., Wu, Q., Qiao, S., Yu, Z., Jin, N., & Yu, B. (2009). Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients' urine by liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 50(5), 939-46. https://doi.org/10.1016/j.jpba.2009.06.032
Yu J, et al. Simultaneous Determination of Phytoestrogens and Key Metabolites in Breast Cancer Patients' Urine By Liquid Chromatography-tandem Mass Spectrometry. J Pharm Biomed Anal. 2009 Dec 5;50(5):939-46. PubMed PMID: 19595530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients' urine by liquid chromatography-tandem mass spectrometry. AU - Yu,Jing, AU - Wu,Qian, AU - Qiao,Shanlei, AU - Yu,Zeping, AU - Jin,Nianzu, AU - Yu,Boyang, Y1 - 2009/06/24/ PY - 2009/02/09/received PY - 2009/06/10/revised PY - 2009/06/11/accepted PY - 2009/7/15/entrez PY - 2009/7/15/pubmed PY - 2010/1/13/medline SP - 939 EP - 46 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 50 IS - 5 N2 - A novel, selective and sensitive liquid chromatography-tandem mass spectrometry method has been developed and validated for the simultaneous determination of phytoestrogens and their key metabolites in human urine in this study. This method includes internal standard (IS) screening, analytical sample preparation procedure establishment, and linear range investigation. The analytical sample was extracted by liquid-liquid extraction from urine sample. The phytoestrogens and related key metabolites were separated with Agilent Zorbax Eclipse XDB-C18 chromatographic column using methanol and water as mobile phase. The Quattro premier MICROMASS mass spectrometer in negative ion selected reaction monitoring (SRM) mode using electrospray ionization was applied to detect the phytoestrogens and key metabolites. To validate the developed liquid chromatography-tandem mass spectrometry method, the intra- and inter-day precisions, specificity, sensitivity, reproducibility, and sample detective concentration range were evaluated. This is the first reported phytoestrogens analysis and validation study that demonstrates the feasibility of using liquid chromatography-electrospray ionization mass spectrometry to simultaneously analyze ten analytes including both phytoestrogens and their key metabolites in urine samples collected for epidemiological studies in human. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/19595530/Simultaneous_determination_of_phytoestrogens_and_key_metabolites_in_breast_cancer_patients'_urine_by_liquid_chromatography_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -