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High-salt loading exacerbates increased retinal content of aquaporins AQP1 and AQP4 in rats with diabetic retinopathy.
Exp Eye Res. 2009 Nov; 89(5):741-7.EE

Abstract

In the neural retina, glial cells control formation of ionic gradients by mediating transmembrane water fluxes through aquaporin (AQP) water channels. Retinal content and immunolocalization of two water channels, AQP1 and AQP4, in the diabetic rat retinas during high-salt loading were examined in this study. Diabetes was induced by an intraperitoneal injection of streptozotocin. Diabetic and control animals were observed after varying lengths of exposure to normal- and high-salt conditions. Ultrathin sections of retinal tissue, stained with uranyl acetate and lead citrate, were photographed using a transmission electron microscope (TEM). Retinal wholemounts were immunostained with AQP1 and AQP4 antibody to detect the immunolocalization changes by confocal microscopy. AQP1 and AQP4 content were evaluated by Western blot analysis. In the retinas of high-salt loading diabetic animals, obviously increased intracellular edema was observed by TEM in ganglion cells and mitochondrial swelling was observed in glial cells. Immunolocalization of AQP1 increased from the posterior to peripheral retina. Western blot results indicated that a high-salt diet may cause increased retinal content of AQP4 and may exacerbate increased retinal content of AQP1 caused by diabetic retinopathy. High-salt loading may increase neural retinal edema in rats with diabetic retinopathy, and altered glial cell mediated water transport via AQP channels in the retina may play an important role in the neural retinal edema formation and resolution.

Authors+Show Affiliations

EENT Hospital, Eye Institute, Fudan University, Fengyang Road 83, Shanghai 200031, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19596320

Citation

Qin, Yaowu, et al. "High-salt Loading Exacerbates Increased Retinal Content of Aquaporins AQP1 and AQP4 in Rats With Diabetic Retinopathy." Experimental Eye Research, vol. 89, no. 5, 2009, pp. 741-7.
Qin Y, Xu G, Fan J, et al. High-salt loading exacerbates increased retinal content of aquaporins AQP1 and AQP4 in rats with diabetic retinopathy. Exp Eye Res. 2009;89(5):741-7.
Qin, Y., Xu, G., Fan, J., Witt, R. E., & Da, C. (2009). High-salt loading exacerbates increased retinal content of aquaporins AQP1 and AQP4 in rats with diabetic retinopathy. Experimental Eye Research, 89(5), 741-7. https://doi.org/10.1016/j.exer.2009.06.020
Qin Y, et al. High-salt Loading Exacerbates Increased Retinal Content of Aquaporins AQP1 and AQP4 in Rats With Diabetic Retinopathy. Exp Eye Res. 2009;89(5):741-7. PubMed PMID: 19596320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-salt loading exacerbates increased retinal content of aquaporins AQP1 and AQP4 in rats with diabetic retinopathy. AU - Qin,Yaowu, AU - Xu,Gezhi, AU - Fan,Jiawen, AU - Witt,Rachel E, AU - Da,Cuidi, Y1 - 2009/07/09/ PY - 2009/04/21/received PY - 2009/06/24/revised PY - 2009/06/26/accepted PY - 2009/7/15/entrez PY - 2009/7/15/pubmed PY - 2010/2/5/medline SP - 741 EP - 7 JF - Experimental eye research JO - Exp Eye Res VL - 89 IS - 5 N2 - In the neural retina, glial cells control formation of ionic gradients by mediating transmembrane water fluxes through aquaporin (AQP) water channels. Retinal content and immunolocalization of two water channels, AQP1 and AQP4, in the diabetic rat retinas during high-salt loading were examined in this study. Diabetes was induced by an intraperitoneal injection of streptozotocin. Diabetic and control animals were observed after varying lengths of exposure to normal- and high-salt conditions. Ultrathin sections of retinal tissue, stained with uranyl acetate and lead citrate, were photographed using a transmission electron microscope (TEM). Retinal wholemounts were immunostained with AQP1 and AQP4 antibody to detect the immunolocalization changes by confocal microscopy. AQP1 and AQP4 content were evaluated by Western blot analysis. In the retinas of high-salt loading diabetic animals, obviously increased intracellular edema was observed by TEM in ganglion cells and mitochondrial swelling was observed in glial cells. Immunolocalization of AQP1 increased from the posterior to peripheral retina. Western blot results indicated that a high-salt diet may cause increased retinal content of AQP4 and may exacerbate increased retinal content of AQP1 caused by diabetic retinopathy. High-salt loading may increase neural retinal edema in rats with diabetic retinopathy, and altered glial cell mediated water transport via AQP channels in the retina may play an important role in the neural retinal edema formation and resolution. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/19596320/High_salt_loading_exacerbates_increased_retinal_content_of_aquaporins_AQP1_and_AQP4_in_rats_with_diabetic_retinopathy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(09)00187-0 DB - PRIME DP - Unbound Medicine ER -