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Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis.

Abstract

BACKGROUND

Both antibody and cell-mediated immune responses are involved in the defence against influenza. In Wegener's granulomatosis (WG), antibody responses to influenza vaccination appear to be similar to those in healthy controls, but cell-mediated responses have not been studied.

OBJECTIVE

To determine whether cell-mediated responses to influenza vaccination in WG vary from those in controls.

METHODS

Twenty-five patients with WG and healthy controls received subunit influenza vaccine. Peripheral blood mononuclear cells were obtained before and 1 month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon gamma (IFN gamma) ELISpot and intracellular cytokine staining for IFN gamma, tumour necrosis factor and interleukin 2.

RESULTS

Before vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. After vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4 T cells increased in patients and controls for A/H1N1 and A/H3N2.

CONCLUSIONS

Cell-mediated responses to influenza vaccination in patients with WG are comparable to those in healthy controls.

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  • Authors+Show Affiliations

    ,

    Department of Rheumatology and Clinical Immunology, University Medical Centre Groningen, University of Groningen, PO Box 30.001, 9700 RB Groningen, The Netherlands. bertholvast@hotmail.com

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    Source

    Annals of the rheumatic diseases 69:5 2010 May pg 924-7

    MeSH

    Adult
    CD4-Positive T-Lymphocytes
    CD8-Positive T-Lymphocytes
    Cytokines
    Female
    Granulomatosis with Polyangiitis
    Humans
    Immunity, Cellular
    Immunosuppressive Agents
    Influenza A Virus, H1N1 Subtype
    Influenza A Virus, H3N2 Subtype
    Influenza Vaccines
    Lymphocyte Activation
    Male
    Middle Aged
    Vaccination

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19596692

    Citation

    Holvast, Albert, et al. "Cell-mediated Immune Responses to Influenza Vaccination in Wegener's Granulomatosis." Annals of the Rheumatic Diseases, vol. 69, no. 5, 2010, pp. 924-7.
    Holvast A, de Haan A, van Assen S, et al. Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis. Ann Rheum Dis. 2010;69(5):924-7.
    Holvast, A., de Haan, A., van Assen, S., Stegeman, C. A., Huitema, M. G., Huckriede, A., ... Bijl, M. (2010). Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis. Annals of the Rheumatic Diseases, 69(5), pp. 924-7. doi:10.1136/ard.2009.112813.
    Holvast A, et al. Cell-mediated Immune Responses to Influenza Vaccination in Wegener's Granulomatosis. Ann Rheum Dis. 2010;69(5):924-7. PubMed PMID: 19596692.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis. AU - Holvast,Albert, AU - de Haan,Aalzen, AU - van Assen,Sander, AU - Stegeman,Coen A, AU - Huitema,Minke G, AU - Huckriede,Anke, AU - Benne,Cornelis A, AU - Westra,Johanna, AU - Palache,Abraham, AU - Wilschut,Jan, AU - Kallenberg,Cees G M, AU - Bijl,Marc, Y1 - 2009/07/12/ PY - 2009/7/15/entrez PY - 2009/7/15/pubmed PY - 2010/6/16/medline SP - 924 EP - 7 JF - Annals of the rheumatic diseases JO - Ann. Rheum. Dis. VL - 69 IS - 5 N2 - BACKGROUND: Both antibody and cell-mediated immune responses are involved in the defence against influenza. In Wegener's granulomatosis (WG), antibody responses to influenza vaccination appear to be similar to those in healthy controls, but cell-mediated responses have not been studied. OBJECTIVE: To determine whether cell-mediated responses to influenza vaccination in WG vary from those in controls. METHODS: Twenty-five patients with WG and healthy controls received subunit influenza vaccine. Peripheral blood mononuclear cells were obtained before and 1 month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon gamma (IFN gamma) ELISpot and intracellular cytokine staining for IFN gamma, tumour necrosis factor and interleukin 2. RESULTS: Before vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. After vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4 T cells increased in patients and controls for A/H1N1 and A/H3N2. CONCLUSIONS: Cell-mediated responses to influenza vaccination in patients with WG are comparable to those in healthy controls. SN - 1468-2060 UR - https://www.unboundmedicine.com/medline/citation/19596692/Cell_mediated_immune_responses_to_influenza_vaccination_in_Wegener's_granulomatosis_ L2 - http://ard.bmj.com/cgi/pmidlookup?view=long&pmid=19596692 DB - PRIME DP - Unbound Medicine ER -