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Effect of adrenotensin on cell proliferation is mediated by angiotensin II in cultured rat mesangial cells.
Acta Pharmacol Sin. 2009 Aug; 30(8):1132-7.AP

Abstract

AIM

Both adrenomedullin (ADM) and adrenotensin (ADT) are derived from the same propeptide precursor, and both act as circulating hormones and local paracrine mediators with multiple biological activities. Compared with ADM, little is known about how ADT achieves its functions. In the present study, we investigated the effect of ADT on cell proliferation and transforming growth factor-beta (TGF-beta) secretion in cultured renal mesangial cells (MCs) and determined whether angiotensin II (Ang II) was involved in mediating this process.

METHODS

Cell proliferation was measured by bromodeoxyuridine (BrdU) incorporation assay, Ang II levels were assayed using an enzyme immunoassay, and real time PCR was used to measure Ang II type 1 (AT1) receptor, Ang II type 2 (AT2) receptor, angiotensinogen (AGT), renin, angiotensin converting enzyme (ACE) and TGF-beta1 mRNA levels. TGF-beta1 and collagen type IV protein levels in cell media were measured using enzyme-linked immunoassays.

RESULTS

ADT treatment induced cell proliferation in a concentration-dependent manner; it also increased the levels of TGF-beta1 mRNA and protein as well as collagen type IV excretion by cultured MCs. ADT treatment increased renin and AGT mRNAs as well as Ang II protein, but did not affect the ACE mRNA level. ADT up-regulated angiotensin AT1 receptor mRNA, but not that of the AT2 receptor. The angiotensin AT1 receptor antagonist losartan blocked the effects of ADT-induced cell proliferation, TGF-beta1 and collagen type IV synthesis and secretion.

CONCLUSION

ADT has a stimulating role in cell proliferation in cultured MCs. Increases in the levels of Ang II and the AT1 receptor after ADT treatment mediate the stimulating effects of ADT on cell proliferation and extracellular matrix synthesis and secretion.

Authors+Show Affiliations

Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19597528

Citation

Xue, Hong, et al. "Effect of Adrenotensin On Cell Proliferation Is Mediated By Angiotensin II in Cultured Rat Mesangial Cells." Acta Pharmacologica Sinica, vol. 30, no. 8, 2009, pp. 1132-7.
Xue H, Yuan P, Zhou L, et al. Effect of adrenotensin on cell proliferation is mediated by angiotensin II in cultured rat mesangial cells. Acta Pharmacol Sin. 2009;30(8):1132-7.
Xue, H., Yuan, P., Zhou, L., Yao, T., Huang, Y., & Lu, L. M. (2009). Effect of adrenotensin on cell proliferation is mediated by angiotensin II in cultured rat mesangial cells. Acta Pharmacologica Sinica, 30(8), 1132-7. https://doi.org/10.1038/aps.2009.103
Xue H, et al. Effect of Adrenotensin On Cell Proliferation Is Mediated By Angiotensin II in Cultured Rat Mesangial Cells. Acta Pharmacol Sin. 2009;30(8):1132-7. PubMed PMID: 19597528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of adrenotensin on cell proliferation is mediated by angiotensin II in cultured rat mesangial cells. AU - Xue,Hong, AU - Yuan,Ping, AU - Zhou,Li, AU - Yao,Tai, AU - Huang,Yu, AU - Lu,Li-min, Y1 - 2009/07/13/ PY - 2009/7/15/entrez PY - 2009/7/15/pubmed PY - 2009/10/23/medline SP - 1132 EP - 7 JF - Acta pharmacologica Sinica JO - Acta Pharmacol. Sin. VL - 30 IS - 8 N2 - AIM: Both adrenomedullin (ADM) and adrenotensin (ADT) are derived from the same propeptide precursor, and both act as circulating hormones and local paracrine mediators with multiple biological activities. Compared with ADM, little is known about how ADT achieves its functions. In the present study, we investigated the effect of ADT on cell proliferation and transforming growth factor-beta (TGF-beta) secretion in cultured renal mesangial cells (MCs) and determined whether angiotensin II (Ang II) was involved in mediating this process. METHODS: Cell proliferation was measured by bromodeoxyuridine (BrdU) incorporation assay, Ang II levels were assayed using an enzyme immunoassay, and real time PCR was used to measure Ang II type 1 (AT1) receptor, Ang II type 2 (AT2) receptor, angiotensinogen (AGT), renin, angiotensin converting enzyme (ACE) and TGF-beta1 mRNA levels. TGF-beta1 and collagen type IV protein levels in cell media were measured using enzyme-linked immunoassays. RESULTS: ADT treatment induced cell proliferation in a concentration-dependent manner; it also increased the levels of TGF-beta1 mRNA and protein as well as collagen type IV excretion by cultured MCs. ADT treatment increased renin and AGT mRNAs as well as Ang II protein, but did not affect the ACE mRNA level. ADT up-regulated angiotensin AT1 receptor mRNA, but not that of the AT2 receptor. The angiotensin AT1 receptor antagonist losartan blocked the effects of ADT-induced cell proliferation, TGF-beta1 and collagen type IV synthesis and secretion. CONCLUSION: ADT has a stimulating role in cell proliferation in cultured MCs. Increases in the levels of Ang II and the AT1 receptor after ADT treatment mediate the stimulating effects of ADT on cell proliferation and extracellular matrix synthesis and secretion. SN - 1745-7254 UR - https://www.unboundmedicine.com/medline/citation/19597528/Effect_of_adrenotensin_on_cell_proliferation_is_mediated_by_angiotensin_II_in_cultured_rat_mesangial_cells_ L2 - http://dx.doi.org/10.1038/aps.2009.103 DB - PRIME DP - Unbound Medicine ER -