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Caenorhabditis elegans lifespan extension caused by treatment with an orally active ROS-generator is dependent on DAF-16 and SIR-2.1.
Biogerontology 2010; 11(2):183-95B

Abstract

In Caenorhabditis elegans pretreatment with juglone, a generator of reactive oxygen species (ROS) provides a subsequently increased ROS-resistance. We investigated whether juglone at low or high concentrations when provided via the oral route in a liquid axenic medium affects normal lifespan of C. elegans. High juglone concentrations led to premature death, low concentrations were tolerated well and caused a prolongation of lifespan. Lifespan extension under moderate oxidative stress was associated with increased expression of small heat-shock protein HSP-16.2, enhanced glutathione levels, and nuclear translocation of DAF-16. Silencing or deletion of DAF-16 prevented the juglone-induced adaptations. RNA-interference for SIR-2.1 had the same effects as the deletion of DAF-16 but did not affect nuclear accumulation of DAF-16. Our studies demonstrate that DAF-16- and SIR-2.1-dependent alterations in gene expression after a ROS challenge lead to a lifespan extension in C. elegans as long as the stressor concentration does not exceed the saturable protective capacity.

Authors+Show Affiliations

Department of Food and Nutrition, Molecular Nutrition Unit, Technical University of Munich, Am Forum 5, Freising, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19597959

Citation

Heidler, Tanja, et al. "Caenorhabditis Elegans Lifespan Extension Caused By Treatment With an Orally Active ROS-generator Is Dependent On DAF-16 and SIR-2.1." Biogerontology, vol. 11, no. 2, 2010, pp. 183-95.
Heidler T, Hartwig K, Daniel H, et al. Caenorhabditis elegans lifespan extension caused by treatment with an orally active ROS-generator is dependent on DAF-16 and SIR-2.1. Biogerontology. 2010;11(2):183-95.
Heidler, T., Hartwig, K., Daniel, H., & Wenzel, U. (2010). Caenorhabditis elegans lifespan extension caused by treatment with an orally active ROS-generator is dependent on DAF-16 and SIR-2.1. Biogerontology, 11(2), pp. 183-95. doi:10.1007/s10522-009-9239-x.
Heidler T, et al. Caenorhabditis Elegans Lifespan Extension Caused By Treatment With an Orally Active ROS-generator Is Dependent On DAF-16 and SIR-2.1. Biogerontology. 2010;11(2):183-95. PubMed PMID: 19597959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caenorhabditis elegans lifespan extension caused by treatment with an orally active ROS-generator is dependent on DAF-16 and SIR-2.1. AU - Heidler,Tanja, AU - Hartwig,Kai, AU - Daniel,Hannelore, AU - Wenzel,Uwe, Y1 - 2009/07/14/ PY - 2009/02/11/received PY - 2009/06/30/accepted PY - 2009/7/15/entrez PY - 2009/7/15/pubmed PY - 2010/5/21/medline SP - 183 EP - 95 JF - Biogerontology JO - Biogerontology VL - 11 IS - 2 N2 - In Caenorhabditis elegans pretreatment with juglone, a generator of reactive oxygen species (ROS) provides a subsequently increased ROS-resistance. We investigated whether juglone at low or high concentrations when provided via the oral route in a liquid axenic medium affects normal lifespan of C. elegans. High juglone concentrations led to premature death, low concentrations were tolerated well and caused a prolongation of lifespan. Lifespan extension under moderate oxidative stress was associated with increased expression of small heat-shock protein HSP-16.2, enhanced glutathione levels, and nuclear translocation of DAF-16. Silencing or deletion of DAF-16 prevented the juglone-induced adaptations. RNA-interference for SIR-2.1 had the same effects as the deletion of DAF-16 but did not affect nuclear accumulation of DAF-16. Our studies demonstrate that DAF-16- and SIR-2.1-dependent alterations in gene expression after a ROS challenge lead to a lifespan extension in C. elegans as long as the stressor concentration does not exceed the saturable protective capacity. SN - 1573-6768 UR - https://www.unboundmedicine.com/medline/citation/19597959/Caenorhabditis_elegans_lifespan_extension_caused_by_treatment_with_an_orally_active_ROS_generator_is_dependent_on_DAF_16_and_SIR_2_1_ L2 - https://doi.org/10.1007/s10522-009-9239-x DB - PRIME DP - Unbound Medicine ER -