Tags

Type your tag names separated by a space and hit enter

Expression analysis identifies FAD2-2 as the olive oleate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil.
J Agric Food Chem. 2009 Jul 22; 57(14):6199-206.JA

Abstract

The effect of ripening stage and water regimen on oleate desaturase gene expression levels in the fruit of different olive (Olea europaea L.) varieties was investigated to elucidate the contribution of each to the linoleic acid content in virgin olive oil. To this end, fatty acid analysis and quantitative real time PCR were performed using distinct olive tissues and different developmental stages from the Picual and Arbequina cultivars. The results showed that the olive FAD2-1, FAD2-2, and FAD6 genes were spatial and temporally regulated. In addition, the data indicated that FAD2-2 seems to be the main gene responsible for the linoleic acid content in the olive fruit mesocarp tissue. This conclusion was also confirmed when the study was extended to Hojiblanca, Picudo, and Manzanilla varieties. With regard to the water regimen, unlike the Picual cultivar, a small increase of linoleic acid was observed in the Arbequina variety cultivated with irrigation, which correlated well with the increase detected for the FAD2-2 gene expression level. All of these data strongly suggest that FAD2-2 is the main gene that determines the linoleic acid content in the virgin olive oil.

Authors+Show Affiliations

Instituto de la Grasa (CSIC), 41012 Sevilla, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19601663

Citation

Hernández, M Luisa, et al. "Expression Analysis Identifies FAD2-2 as the Olive Oleate Desaturase Gene Mainly Responsible for the Linoleic Acid Content in Virgin Olive Oil." Journal of Agricultural and Food Chemistry, vol. 57, no. 14, 2009, pp. 6199-206.
Hernández ML, Padilla MN, Mancha M, et al. Expression analysis identifies FAD2-2 as the olive oleate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil. J Agric Food Chem. 2009;57(14):6199-206.
Hernández, M. L., Padilla, M. N., Mancha, M., & Martínez-Rivas, J. M. (2009). Expression analysis identifies FAD2-2 as the olive oleate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil. Journal of Agricultural and Food Chemistry, 57(14), 6199-206. https://doi.org/10.1021/jf900678z
Hernández ML, et al. Expression Analysis Identifies FAD2-2 as the Olive Oleate Desaturase Gene Mainly Responsible for the Linoleic Acid Content in Virgin Olive Oil. J Agric Food Chem. 2009 Jul 22;57(14):6199-206. PubMed PMID: 19601663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis identifies FAD2-2 as the olive oleate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil. AU - Hernández,M Luisa, AU - Padilla,María N, AU - Mancha,Manuel, AU - Martínez-Rivas,José M, PY - 2009/7/16/entrez PY - 2009/7/16/pubmed PY - 2009/11/17/medline SP - 6199 EP - 206 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 14 N2 - The effect of ripening stage and water regimen on oleate desaturase gene expression levels in the fruit of different olive (Olea europaea L.) varieties was investigated to elucidate the contribution of each to the linoleic acid content in virgin olive oil. To this end, fatty acid analysis and quantitative real time PCR were performed using distinct olive tissues and different developmental stages from the Picual and Arbequina cultivars. The results showed that the olive FAD2-1, FAD2-2, and FAD6 genes were spatial and temporally regulated. In addition, the data indicated that FAD2-2 seems to be the main gene responsible for the linoleic acid content in the olive fruit mesocarp tissue. This conclusion was also confirmed when the study was extended to Hojiblanca, Picudo, and Manzanilla varieties. With regard to the water regimen, unlike the Picual cultivar, a small increase of linoleic acid was observed in the Arbequina variety cultivated with irrigation, which correlated well with the increase detected for the FAD2-2 gene expression level. All of these data strongly suggest that FAD2-2 is the main gene that determines the linoleic acid content in the virgin olive oil. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19601663/Expression_analysis_identifies_FAD2_2_as_the_olive_oleate_desaturase_gene_mainly_responsible_for_the_linoleic_acid_content_in_virgin_olive_oil_ L2 - https://doi.org/10.1021/jf900678z DB - PRIME DP - Unbound Medicine ER -