Tags

Type your tag names separated by a space and hit enter

Capn8 promoter directs the expression of Cre recombinase in gastric pit cells of transgenic mice.
Genesis. 2009 Oct; 47(10):674-9.G

Abstract

Gastric pit cells are high-turnover epithelial cells of the gastric mucosa. They secrete mucus to protect the gastric epithelium from acid and pepsin. To investigate the genetic mechanisms underlying the physiological functions of gastric pit cells, we generated a transgenic mouse line, namely, Capn8-Cre, in which the expression of Cre recombinase was controlled by the promoter of the intracellular Ca(2+)-regulated cysteine protease calpain-8. To test the tissue distribution and excision activity of Cre recombinase, the Capn8-Cre transgenic mice were bred with the ROSA26 reporter strain and a mouse strain that carries Smad4 conditional alleles (Smad4(Co/Co)). Multiple-tissue PCR and LacZ staining demonstrated that Capn8-Cre transgenic mouse expressed Cre recombinase in the gastric pit cells. Cre recombinase activity was also detected in the liver and skin tissues. These data suggest that the Capn8-Cre mouse line described here could be used to dissect gene function in gastric pit cells.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, State Key Laboratory of Proteomics, Institute of Biotechnology, Beijing, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19603510

Citation

Zhao, Zengming, et al. "Capn8 Promoter Directs the Expression of Cre Recombinase in Gastric Pit Cells of Transgenic Mice." Genesis (New York, N.Y. : 2000), vol. 47, no. 10, 2009, pp. 674-9.
Zhao Z, Sun Y, Hou N, et al. Capn8 promoter directs the expression of Cre recombinase in gastric pit cells of transgenic mice. Genesis. 2009;47(10):674-9.
Zhao, Z., Sun, Y., Hou, N., Teng, Y., Wang, Y., & Yang, X. (2009). Capn8 promoter directs the expression of Cre recombinase in gastric pit cells of transgenic mice. Genesis (New York, N.Y. : 2000), 47(10), 674-9. https://doi.org/10.1002/dvg.20552
Zhao Z, et al. Capn8 Promoter Directs the Expression of Cre Recombinase in Gastric Pit Cells of Transgenic Mice. Genesis. 2009;47(10):674-9. PubMed PMID: 19603510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capn8 promoter directs the expression of Cre recombinase in gastric pit cells of transgenic mice. AU - Zhao,Zengming, AU - Sun,Yanxun, AU - Hou,Ning, AU - Teng,Yan, AU - Wang,Youliang, AU - Yang,Xiao, PY - 2009/7/16/entrez PY - 2009/7/16/pubmed PY - 2010/2/10/medline SP - 674 EP - 9 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 47 IS - 10 N2 - Gastric pit cells are high-turnover epithelial cells of the gastric mucosa. They secrete mucus to protect the gastric epithelium from acid and pepsin. To investigate the genetic mechanisms underlying the physiological functions of gastric pit cells, we generated a transgenic mouse line, namely, Capn8-Cre, in which the expression of Cre recombinase was controlled by the promoter of the intracellular Ca(2+)-regulated cysteine protease calpain-8. To test the tissue distribution and excision activity of Cre recombinase, the Capn8-Cre transgenic mice were bred with the ROSA26 reporter strain and a mouse strain that carries Smad4 conditional alleles (Smad4(Co/Co)). Multiple-tissue PCR and LacZ staining demonstrated that Capn8-Cre transgenic mouse expressed Cre recombinase in the gastric pit cells. Cre recombinase activity was also detected in the liver and skin tissues. These data suggest that the Capn8-Cre mouse line described here could be used to dissect gene function in gastric pit cells. SN - 1526-968X UR - https://www.unboundmedicine.com/medline/citation/19603510/Capn8_promoter_directs_the_expression_of_Cre_recombinase_in_gastric_pit_cells_of_transgenic_mice_ L2 - https://doi.org/10.1002/dvg.20552 DB - PRIME DP - Unbound Medicine ER -