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Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus.
Insect Mol Biol. 2009 Aug; 18(4):531-9.IM

Abstract

Ixodes persulcatus is the primary vector for human tick-borne diseases in Japan. A cDNA library was constructed from whole body homogenates of fed nymphs of I. persulcatus. From this library, one cDNA encoding defensin-like antimicrobial peptide was identified. The amino-acid sequence showed high similarity to those of the defensins of other ticks and arthropods. I. persulcatus defensin mRNA transcripts were detected at all life cycle stages of fed ticks and found to be predominantly expressed in the midguts of adult female ticks, but not in the salivary glands, a finding corroborated by Western blotting analysis. To investigate the function of I. persulcatus defensin, we examined its antibacterial activity by evaluation of growth of several bacterial strains in the presence of the synthetic peptide. The defensin from I. persulcatus markedly inhibited the growth of Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis and Corynebacterium renale, but not Gram-negative bacteria except Escherichia coli O157. In conclusion, these results suggest that I. persulcatus defensin may be playing a significant role in the defence against microbes from bloodmeals.

Authors+Show Affiliations

Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19604312

Citation

Saito, Y, et al. "Identification and Characterization of Antimicrobial Peptide, Defensin, in the Taiga Tick, Ixodes Persulcatus." Insect Molecular Biology, vol. 18, no. 4, 2009, pp. 531-9.
Saito Y, Konnai S, Yamada S, et al. Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus. Insect Mol Biol. 2009;18(4):531-9.
Saito, Y., Konnai, S., Yamada, S., Imamura, S., Nishikado, H., Ito, T., Onuma, M., & Ohashi, K. (2009). Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus. Insect Molecular Biology, 18(4), 531-9. https://doi.org/10.1111/j.1365-2583.2009.00897.x
Saito Y, et al. Identification and Characterization of Antimicrobial Peptide, Defensin, in the Taiga Tick, Ixodes Persulcatus. Insect Mol Biol. 2009;18(4):531-9. PubMed PMID: 19604312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus. AU - Saito,Y, AU - Konnai,S, AU - Yamada,S, AU - Imamura,S, AU - Nishikado,H, AU - Ito,T, AU - Onuma,M, AU - Ohashi,K, PY - 2009/7/17/entrez PY - 2009/7/17/pubmed PY - 2009/9/15/medline SP - 531 EP - 9 JF - Insect molecular biology JO - Insect Mol. Biol. VL - 18 IS - 4 N2 - Ixodes persulcatus is the primary vector for human tick-borne diseases in Japan. A cDNA library was constructed from whole body homogenates of fed nymphs of I. persulcatus. From this library, one cDNA encoding defensin-like antimicrobial peptide was identified. The amino-acid sequence showed high similarity to those of the defensins of other ticks and arthropods. I. persulcatus defensin mRNA transcripts were detected at all life cycle stages of fed ticks and found to be predominantly expressed in the midguts of adult female ticks, but not in the salivary glands, a finding corroborated by Western blotting analysis. To investigate the function of I. persulcatus defensin, we examined its antibacterial activity by evaluation of growth of several bacterial strains in the presence of the synthetic peptide. The defensin from I. persulcatus markedly inhibited the growth of Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis and Corynebacterium renale, but not Gram-negative bacteria except Escherichia coli O157. In conclusion, these results suggest that I. persulcatus defensin may be playing a significant role in the defence against microbes from bloodmeals. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/19604312/Identification_and_characterization_of_antimicrobial_peptide_defensin_in_the_taiga_tick_Ixodes_persulcatus_ L2 - https://doi.org/10.1111/j.1365-2583.2009.00897.x DB - PRIME DP - Unbound Medicine ER -