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Novel molecularly imprinted polymers for the selective extraction and determination of metoclopramide in human serum and urine samples using high-performance liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15; 877(24):2537-44.JC

Abstract

This work was performed in order to study the possibilities in using molecularly imprinted polymers (MIPs) as sorbent material in solid-phase extraction (MISPE) for the sample clean-up technique for the determination of metoclopramide (MCP) in biological fluids. The effective factors influencing the bulk polymerization have been studied. Molecular recognition properties, binding capability and selectivity of the molecularly imprinted polymers (MIPs) were evaluated and the results revealed the obtained MIPs have high affinity for MCP in aqueous environment. The optimal conditions for solid-phase extraction (SPE) consisted of conditioning with 1 mL of methanol and 1 mL of deionized water at neutral pH, loading with 1 mL of the water sample (50 microg L(-1)) at pH 8.5, washing using 1 mL of acetone and elution with 2x 1 mL methanol/acetic acid (10/1, v/v). After optimization of SPE procedure, the MIP was then directly used to selectively extract the target drug from human serum and urine with an extraction recovery of more than 90%. Chromatograms of the eluate solutions show an efficient clean-up, which supports the potential of MISPE for clean-up of trace amounts of MCP from serum and urine. The limits of detection of MCP in human serum and urine were 3 and 1.2 microg L(-1), respectively.

Authors+Show Affiliations

Department of Chemistry, Amirkabir University of Technology, Tehran, Iran. mehranjavanbakht@gmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19604730

Citation

Javanbakht, Mehran, et al. "Novel Molecularly Imprinted Polymers for the Selective Extraction and Determination of Metoclopramide in Human Serum and Urine Samples Using High-performance Liquid Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 24, 2009, pp. 2537-44.
Javanbakht M, Shaabani N, Akbari-Adergani B. Novel molecularly imprinted polymers for the selective extraction and determination of metoclopramide in human serum and urine samples using high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(24):2537-44.
Javanbakht, M., Shaabani, N., & Akbari-Adergani, B. (2009). Novel molecularly imprinted polymers for the selective extraction and determination of metoclopramide in human serum and urine samples using high-performance liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(24), 2537-44. https://doi.org/10.1016/j.jchromb.2009.06.030
Javanbakht M, Shaabani N, Akbari-Adergani B. Novel Molecularly Imprinted Polymers for the Selective Extraction and Determination of Metoclopramide in Human Serum and Urine Samples Using High-performance Liquid Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15;877(24):2537-44. PubMed PMID: 19604730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel molecularly imprinted polymers for the selective extraction and determination of metoclopramide in human serum and urine samples using high-performance liquid chromatography. AU - Javanbakht,Mehran, AU - Shaabani,Narges, AU - Akbari-Adergani,Behrouz, Y1 - 2009/06/27/ PY - 2009/04/07/received PY - 2009/06/20/revised PY - 2009/06/22/accepted PY - 2009/7/17/entrez PY - 2009/7/17/pubmed PY - 2009/9/25/medline SP - 2537 EP - 44 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 877 IS - 24 N2 - This work was performed in order to study the possibilities in using molecularly imprinted polymers (MIPs) as sorbent material in solid-phase extraction (MISPE) for the sample clean-up technique for the determination of metoclopramide (MCP) in biological fluids. The effective factors influencing the bulk polymerization have been studied. Molecular recognition properties, binding capability and selectivity of the molecularly imprinted polymers (MIPs) were evaluated and the results revealed the obtained MIPs have high affinity for MCP in aqueous environment. The optimal conditions for solid-phase extraction (SPE) consisted of conditioning with 1 mL of methanol and 1 mL of deionized water at neutral pH, loading with 1 mL of the water sample (50 microg L(-1)) at pH 8.5, washing using 1 mL of acetone and elution with 2x 1 mL methanol/acetic acid (10/1, v/v). After optimization of SPE procedure, the MIP was then directly used to selectively extract the target drug from human serum and urine with an extraction recovery of more than 90%. Chromatograms of the eluate solutions show an efficient clean-up, which supports the potential of MISPE for clean-up of trace amounts of MCP from serum and urine. The limits of detection of MCP in human serum and urine were 3 and 1.2 microg L(-1), respectively. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/19604730/Novel_molecularly_imprinted_polymers_for_the_selective_extraction_and_determination_of_metoclopramide_in_human_serum_and_urine_samples_using_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00459-0 DB - PRIME DP - Unbound Medicine ER -