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Amplified electrochemical aptasensor for thrombin based on bio-barcode method.
Biosens Bioelectron. 2009 Sep 15; 25(1):259-62.BB

Abstract

In the present study, an electrochemical aptasensor for highly sensitive detection of thrombin was developed based on bio-barcode amplification assay. For this proposed aptasensor, capture DNA aptamerI was immobilized on the Au electrode. The functional Au nanoparticles (DNA-AuNPs) are loaded with barcode binding DNA and aptamerII. Through the specific recognition for thrombin, a sandwich format of Au/aptamerI/thrombin/DNA-AuNPs was fabricated. After hybridization with the PbSNPs-labeled barcode DNA, the assembled sensor was obtained. The concentration of thrombin was monitored based on the concentration of lead ions dissolved through differential pulse anodic stripping voltammetric (DPASV). Under optimum conditions, a detection limit of 6.2x10(-15) mol L(-1) (M) thrombin was achieved. In addition, the sensor exhibited excellent selectivity against other proteins.

Authors+Show Affiliations

Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19608403

Citation

Zhang, Xiaoru, et al. "Amplified Electrochemical Aptasensor for Thrombin Based On Bio-barcode Method." Biosensors & Bioelectronics, vol. 25, no. 1, 2009, pp. 259-62.
Zhang X, Qi B, Li Y, et al. Amplified electrochemical aptasensor for thrombin based on bio-barcode method. Biosens Bioelectron. 2009;25(1):259-62.
Zhang, X., Qi, B., Li, Y., & Zhang, S. (2009). Amplified electrochemical aptasensor for thrombin based on bio-barcode method. Biosensors & Bioelectronics, 25(1), 259-62. https://doi.org/10.1016/j.bios.2009.06.026
Zhang X, et al. Amplified Electrochemical Aptasensor for Thrombin Based On Bio-barcode Method. Biosens Bioelectron. 2009 Sep 15;25(1):259-62. PubMed PMID: 19608403.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amplified electrochemical aptasensor for thrombin based on bio-barcode method. AU - Zhang,Xiaoru, AU - Qi,Baoping, AU - Li,Ying, AU - Zhang,Shusheng, Y1 - 2009/06/24/ PY - 2009/05/07/received PY - 2009/06/15/revised PY - 2009/06/16/accepted PY - 2009/7/18/entrez PY - 2009/7/18/pubmed PY - 2009/12/16/medline SP - 259 EP - 62 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 1 N2 - In the present study, an electrochemical aptasensor for highly sensitive detection of thrombin was developed based on bio-barcode amplification assay. For this proposed aptasensor, capture DNA aptamerI was immobilized on the Au electrode. The functional Au nanoparticles (DNA-AuNPs) are loaded with barcode binding DNA and aptamerII. Through the specific recognition for thrombin, a sandwich format of Au/aptamerI/thrombin/DNA-AuNPs was fabricated. After hybridization with the PbSNPs-labeled barcode DNA, the assembled sensor was obtained. The concentration of thrombin was monitored based on the concentration of lead ions dissolved through differential pulse anodic stripping voltammetric (DPASV). Under optimum conditions, a detection limit of 6.2x10(-15) mol L(-1) (M) thrombin was achieved. In addition, the sensor exhibited excellent selectivity against other proteins. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19608403/Amplified_electrochemical_aptasensor_for_thrombin_based_on_bio_barcode_method_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00342-X DB - PRIME DP - Unbound Medicine ER -