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A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L.

Abstract

INTRODUCTION

Cannabis and cannabinoid based medicines are currently under serious investigation for legitimate development as medicinal agents, necessitating new low-cost, high-throughput analytical methods for quality control.

OBJECTIVE

The goal of this study was to develop and validate, according to ICH guidelines, a simple rapid HPTLC method for the quantification of Delta(9)-tetrahydrocannabinol (Delta(9)-THC) and qualitative analysis of other main neutral cannabinoids found in cannabis.

METHODOLOGY

The method was developed and validated with the use of pure cannabinoid reference standards and two medicinal cannabis cultivars. Accuracy was determined by comparing results obtained from the HTPLC method with those obtained from a validated HPLC method.

RESULTS

Delta(9)-THC gives linear calibration curves in the range of 50-500 ng at 206 nm with a linear regression of y = 11.858x + 125.99 and r(2) = 0.9968.

CONCLUSION

Results have shown that the HPTLC method is reproducible and accurate for the quantification of Delta(9)-THC in cannabis. The method is also useful for the qualitative screening of the main neutral cannabinoids found in cannabis cultivars.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Division of Pharmacognosy, Institute of Biology, Leiden University, Leiden, The Netherlands. jtfische@gmail.com

    , ,

    Source

    MeSH

    Cannabinoids
    Cannabis
    Chromatography, High Pressure Liquid
    Chromatography, Thin Layer
    Densitometry
    Dronabinol
    Flowers
    Linear Models
    Molecular Structure
    Reproducibility of Results
    Species Specificity

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19609880

    Citation

    TY - JOUR T1 - A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L. AU - Fischedick,Justin T, AU - Glas,Ronald, AU - Hazekamp,Arno, AU - Verpoorte,Rob, PY - 2009/7/18/entrez PY - 2009/7/18/pubmed PY - 2010/3/3/medline SP - 421 EP - 6 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 20 IS - 5 N2 - INTRODUCTION: Cannabis and cannabinoid based medicines are currently under serious investigation for legitimate development as medicinal agents, necessitating new low-cost, high-throughput analytical methods for quality control. OBJECTIVE: The goal of this study was to develop and validate, according to ICH guidelines, a simple rapid HPTLC method for the quantification of Delta(9)-tetrahydrocannabinol (Delta(9)-THC) and qualitative analysis of other main neutral cannabinoids found in cannabis. METHODOLOGY: The method was developed and validated with the use of pure cannabinoid reference standards and two medicinal cannabis cultivars. Accuracy was determined by comparing results obtained from the HTPLC method with those obtained from a validated HPLC method. RESULTS: Delta(9)-THC gives linear calibration curves in the range of 50-500 ng at 206 nm with a linear regression of y = 11.858x + 125.99 and r(2) = 0.9968. CONCLUSION: Results have shown that the HPTLC method is reproducible and accurate for the quantification of Delta(9)-THC in cannabis. The method is also useful for the qualitative screening of the main neutral cannabinoids found in cannabis cultivars. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/19609880/abstract/A_qualitative_and_quantitative_HPTLC_densitometry_method_for_the_analysis_of_cannabinoids_in_Cannabis_sativa_L_ L2 - https://doi.org/10.1002/pca.1143 ER -