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Separation and determination of clenbuterol by HPLC using a vancomycin chiral stationary phase.
J AOAC Int. 2009 May-Jun; 92(3):824-9.JA

Abstract

Enantiomers of clenbuterol were separated by a new HPLC method on a chiral column. Enantiomeric resolution was achieved on a vancomycyin macrocyclic antibiotic chiral stationary phase known as chirobiotic V with UV detection at 247 nm. The polar ionic mobile phase consisting of methanol-triethylamine-glacial acetic acid (100 + 0.05 + 0.025, v/v/v), was used at a flow rate of 1.0 mL/min. The method was validated for linearity, accuracy, precision, and robustness. Standard linear calibration curves were established for the R-(-) and S-(+) enantiomers over the range of 0.2-20 microg/mL, and an average recovery of 98.0% and a mean relative standard deviation of 1.5% were obtained at 5.0 microg/mL. The lower limit of detection was 0.05 microg/mL for each enantiomer. The mean recovery for R-(-) and S-(+)-clenbuterol enantiomers from plasma was 91.0-97.0% at 0.20-20 microg/mL. The method was successfully used to identify and quantify the clenbuterol enantiomers in human plasma.

Authors+Show Affiliations

King Saud University, College of Pharmacy, Pharmaceutical Chemistry Department, PO Box 2457, Riyadh 11451, Saudi Arabia. gamal_most@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19610374

Citation

Mostafa, Gamal A E., et al. "Separation and Determination of Clenbuterol By HPLC Using a Vancomycin Chiral Stationary Phase." Journal of AOAC International, vol. 92, no. 3, 2009, pp. 824-9.
Mostafa GA, Hefnawy MM, El-Majed A. Separation and determination of clenbuterol by HPLC using a vancomycin chiral stationary phase. J AOAC Int. 2009;92(3):824-9.
Mostafa, G. A., Hefnawy, M. M., & El-Majed, A. (2009). Separation and determination of clenbuterol by HPLC using a vancomycin chiral stationary phase. Journal of AOAC International, 92(3), 824-9.
Mostafa GA, Hefnawy MM, El-Majed A. Separation and Determination of Clenbuterol By HPLC Using a Vancomycin Chiral Stationary Phase. J AOAC Int. 2009 May-Jun;92(3):824-9. PubMed PMID: 19610374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of clenbuterol by HPLC using a vancomycin chiral stationary phase. AU - Mostafa,Gamal A E, AU - Hefnawy,Mohammed M, AU - El-Majed,Abdulrahman, PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2009/8/12/medline SP - 824 EP - 9 JF - Journal of AOAC International JO - J AOAC Int VL - 92 IS - 3 N2 - Enantiomers of clenbuterol were separated by a new HPLC method on a chiral column. Enantiomeric resolution was achieved on a vancomycyin macrocyclic antibiotic chiral stationary phase known as chirobiotic V with UV detection at 247 nm. The polar ionic mobile phase consisting of methanol-triethylamine-glacial acetic acid (100 + 0.05 + 0.025, v/v/v), was used at a flow rate of 1.0 mL/min. The method was validated for linearity, accuracy, precision, and robustness. Standard linear calibration curves were established for the R-(-) and S-(+) enantiomers over the range of 0.2-20 microg/mL, and an average recovery of 98.0% and a mean relative standard deviation of 1.5% were obtained at 5.0 microg/mL. The lower limit of detection was 0.05 microg/mL for each enantiomer. The mean recovery for R-(-) and S-(+)-clenbuterol enantiomers from plasma was 91.0-97.0% at 0.20-20 microg/mL. The method was successfully used to identify and quantify the clenbuterol enantiomers in human plasma. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/19610374/Separation_and_determination_of_clenbuterol_by_HPLC_using_a_vancomycin_chiral_stationary_phase_ DB - PRIME DP - Unbound Medicine ER -
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