Tags

Type your tag names separated by a space and hit enter

Neuronal and glial localization of the cannabinoid-1 receptor in the superficial spinal dorsal horn of the rodent spinal cord.
Eur J Neurosci. 2009 Jul; 30(2):251-62.EJ

Abstract

A long line of experimental evidence indicates that endogenous cannabinoid mechanisms play important roles in nociceptive information processing in various areas of the nervous system including the spinal cord. Although it is extensively documented that the cannabinoid-1 receptor (CB(1)-R) is strongly expressed in the superficial spinal dorsal horn, its cellular distribution is poorly defined, hampering our interpretation of the effect of cannabinoids on pain processing spinal neural circuits. Thus, we investigated the cellular distribution of CB(1)-Rs in laminae I and II of the rodent spinal dorsal horn with immunocytochemical methods. Axonal varicosities revealed a strong immunoreactivity for CB(1)-R, but no CB(1)-R expression was observed on dendrites and perikarya of neurons. Investigating the co-localization of CB(1)-R with markers of peptidergic and non-peptidergic primary afferents, and axon terminals of putative glutamatergic and GABAergic spinal neurons we found that nearly half of the peptidergic (immunoreactive for calcitonin gene-related peptide) and more than 20% of the non-peptidergic (binding isolectin B4) nociceptive primary afferents, more than one-third and approximately 20% of the axon terminals of putative glutamatergic (immunoreactive for vesicular glutamate transporter 2) and GABAergic (immunoreactive for glutamic acid decarboxylase; GAD65 and/or GAD67) spinal interneurons, respectively, were positively stained for CB(1)-R. In addition to axon terminals, almost half of the astrocytic (immunoreactive for glial fibrillary acidic protein) and nearly 80% of microglial (immunoreactive for CD11b) profiles were also immunolabeled for CB(1)-R. The findings suggest that the activity-dependent release of endogenous cannabinoids activates a complex signaling mechanism in pain processing spinal neural circuits into which both neurons and glial cells may contribute.

Authors+Show Affiliations

Department of Anatomy, Histology and Embryology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19614976

Citation

Hegyi, Zoltán, et al. "Neuronal and Glial Localization of the Cannabinoid-1 Receptor in the Superficial Spinal Dorsal Horn of the Rodent Spinal Cord." The European Journal of Neuroscience, vol. 30, no. 2, 2009, pp. 251-62.
Hegyi Z, Kis G, Holló K, et al. Neuronal and glial localization of the cannabinoid-1 receptor in the superficial spinal dorsal horn of the rodent spinal cord. Eur J Neurosci. 2009;30(2):251-62.
Hegyi, Z., Kis, G., Holló, K., Ledent, C., & Antal, M. (2009). Neuronal and glial localization of the cannabinoid-1 receptor in the superficial spinal dorsal horn of the rodent spinal cord. The European Journal of Neuroscience, 30(2), 251-62. https://doi.org/10.1111/j.1460-9568.2009.06816.x
Hegyi Z, et al. Neuronal and Glial Localization of the Cannabinoid-1 Receptor in the Superficial Spinal Dorsal Horn of the Rodent Spinal Cord. Eur J Neurosci. 2009;30(2):251-62. PubMed PMID: 19614976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal and glial localization of the cannabinoid-1 receptor in the superficial spinal dorsal horn of the rodent spinal cord. AU - Hegyi,Zoltán, AU - Kis,Gréta, AU - Holló,Krisztina, AU - Ledent,Catherine, AU - Antal,Miklós, Y1 - 2009/07/15/ PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2010/1/9/medline SP - 251 EP - 62 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 30 IS - 2 N2 - A long line of experimental evidence indicates that endogenous cannabinoid mechanisms play important roles in nociceptive information processing in various areas of the nervous system including the spinal cord. Although it is extensively documented that the cannabinoid-1 receptor (CB(1)-R) is strongly expressed in the superficial spinal dorsal horn, its cellular distribution is poorly defined, hampering our interpretation of the effect of cannabinoids on pain processing spinal neural circuits. Thus, we investigated the cellular distribution of CB(1)-Rs in laminae I and II of the rodent spinal dorsal horn with immunocytochemical methods. Axonal varicosities revealed a strong immunoreactivity for CB(1)-R, but no CB(1)-R expression was observed on dendrites and perikarya of neurons. Investigating the co-localization of CB(1)-R with markers of peptidergic and non-peptidergic primary afferents, and axon terminals of putative glutamatergic and GABAergic spinal neurons we found that nearly half of the peptidergic (immunoreactive for calcitonin gene-related peptide) and more than 20% of the non-peptidergic (binding isolectin B4) nociceptive primary afferents, more than one-third and approximately 20% of the axon terminals of putative glutamatergic (immunoreactive for vesicular glutamate transporter 2) and GABAergic (immunoreactive for glutamic acid decarboxylase; GAD65 and/or GAD67) spinal interneurons, respectively, were positively stained for CB(1)-R. In addition to axon terminals, almost half of the astrocytic (immunoreactive for glial fibrillary acidic protein) and nearly 80% of microglial (immunoreactive for CD11b) profiles were also immunolabeled for CB(1)-R. The findings suggest that the activity-dependent release of endogenous cannabinoids activates a complex signaling mechanism in pain processing spinal neural circuits into which both neurons and glial cells may contribute. SN - 1460-9568 UR - https://www.unboundmedicine.com/medline/citation/19614976/Neuronal_and_glial_localization_of_the_cannabinoid_1_receptor_in_the_superficial_spinal_dorsal_horn_of_the_rodent_spinal_cord_ L2 - https://doi.org/10.1111/j.1460-9568.2009.06816.x DB - PRIME DP - Unbound Medicine ER -