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Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens.
J Insect Physiol. 2009 Nov; 55(11):997-1002.JI

Abstract

Trehalose is the main blood sugar of insects, and the enzyme trehalase is involved in energy metabolism and controlling trehalose levels in cells. Two forms (soluble and membrane-bound) of trehalase and the corresponding genes (NlTre-1 and NlTre-2) were identified from the brown planthopper, Nilaparvata lugens. Both NlTre-1 and NlTre-2 contain trehalase signature motifs, and NlTre-2 contains a putative transmembrane domain. Comparison of trehalase activity and gene mRNA level at different developmental stages, or following application of 20-hydroxyecdysone (20E), suggests that NlTre-1 and NlTre-2 encode a soluble trehalase and a membrane-bound trehalase respectively. Soluble trehalase activity accounted for the majority of total trehalase activity in N. lugens. Only soluble trehalase activity and NlTre-1 mRNA level could be induced by 20E. Additionally, only soluble trehalase activity was significantly higher in macropterous individuals than in brachypterous morphs. These results indicate that only soluble trehalase is differentially expressed between macropterous and brachypterous individuals and is more responsive to hormone stimulus.

Authors+Show Affiliations

Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19615372

Citation

Gu, Jianhua, et al. "Characterization of Putative Soluble and Membrane-bound Trehalases in a Hemipteran Insect, Nilaparvata Lugens." Journal of Insect Physiology, vol. 55, no. 11, 2009, pp. 997-1002.
Gu J, Shao Y, Zhang C, et al. Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens. J Insect Physiol. 2009;55(11):997-1002.
Gu, J., Shao, Y., Zhang, C., Liu, Z., & Zhang, Y. (2009). Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens. Journal of Insect Physiology, 55(11), 997-1002. https://doi.org/10.1016/j.jinsphys.2009.07.003
Gu J, et al. Characterization of Putative Soluble and Membrane-bound Trehalases in a Hemipteran Insect, Nilaparvata Lugens. J Insect Physiol. 2009;55(11):997-1002. PubMed PMID: 19615372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens. AU - Gu,Jianhua, AU - Shao,Ying, AU - Zhang,Chengwei, AU - Liu,Zewen, AU - Zhang,Yongjun, Y1 - 2009/07/24/ PY - 2009/04/23/received PY - 2009/07/07/revised PY - 2009/07/07/accepted PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2009/12/16/medline SP - 997 EP - 1002 JF - Journal of insect physiology JO - J. Insect Physiol. VL - 55 IS - 11 N2 - Trehalose is the main blood sugar of insects, and the enzyme trehalase is involved in energy metabolism and controlling trehalose levels in cells. Two forms (soluble and membrane-bound) of trehalase and the corresponding genes (NlTre-1 and NlTre-2) were identified from the brown planthopper, Nilaparvata lugens. Both NlTre-1 and NlTre-2 contain trehalase signature motifs, and NlTre-2 contains a putative transmembrane domain. Comparison of trehalase activity and gene mRNA level at different developmental stages, or following application of 20-hydroxyecdysone (20E), suggests that NlTre-1 and NlTre-2 encode a soluble trehalase and a membrane-bound trehalase respectively. Soluble trehalase activity accounted for the majority of total trehalase activity in N. lugens. Only soluble trehalase activity and NlTre-1 mRNA level could be induced by 20E. Additionally, only soluble trehalase activity was significantly higher in macropterous individuals than in brachypterous morphs. These results indicate that only soluble trehalase is differentially expressed between macropterous and brachypterous individuals and is more responsive to hormone stimulus. SN - 1879-1611 UR - https://www.unboundmedicine.com/medline/citation/19615372/Characterization_of_putative_soluble_and_membrane_bound_trehalases_in_a_hemipteran_insect_Nilaparvata_lugens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(09)00256-X DB - PRIME DP - Unbound Medicine ER -