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Molecular characterization of Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolates in Egypt.
Microbiol Res 2010; 165(2):152-62MR

Abstract

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+ methicillin-susceptible S. aureus and PVL+CA-MRSA from different countries, and investigated the allelic variations among their lukS-PV and lukF-PV gene sequences. The prevalence of PVL+MRSA was 19.04%. They belonged to different genetic clones with multi-locus sequence types (MLST) 30, 80, and the novel type 1010. ST80 strains showed unique antibiotic resistance profile that was distinguishable from the European ST80 clone: they had no resistance to tetracycline and fusidic acid. Two nonsynonymous substitutions in the lukS-PV gene region and two haplotype variants among these isolates were detected. We revealed that PVL is a good marker for CA-MRSA infections and that PVL+ isolates belonged to different genotypes.

Authors+Show Affiliations

Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. shymaa21@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19616420

Citation

Enany, Shymaa, et al. "Molecular Characterization of Panton-Valentine Leukocidin-positive Community-acquired Methicillin-resistant Staphylococcus Aureus Isolates in Egypt." Microbiological Research, vol. 165, no. 2, 2010, pp. 152-62.
Enany S, Yaoita E, Yoshida Y, et al. Molecular characterization of Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolates in Egypt. Microbiol Res. 2010;165(2):152-62.
Enany, S., Yaoita, E., Yoshida, Y., Enany, M., & Yamamoto, T. (2010). Molecular characterization of Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolates in Egypt. Microbiological Research, 165(2), pp. 152-62. doi:10.1016/j.micres.2009.03.005.
Enany S, et al. Molecular Characterization of Panton-Valentine Leukocidin-positive Community-acquired Methicillin-resistant Staphylococcus Aureus Isolates in Egypt. Microbiol Res. 2010 Feb 28;165(2):152-62. PubMed PMID: 19616420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolates in Egypt. AU - Enany,Shymaa, AU - Yaoita,Eishin, AU - Yoshida,Yutaka, AU - Enany,Mohamed, AU - Yamamoto,Tadashi, Y1 - 2009/07/17/ PY - 2009/02/03/received PY - 2009/03/23/revised PY - 2009/03/29/accepted PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2010/4/8/medline SP - 152 EP - 62 JF - Microbiological research JO - Microbiol. Res. VL - 165 IS - 2 N2 - Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+ methicillin-susceptible S. aureus and PVL+CA-MRSA from different countries, and investigated the allelic variations among their lukS-PV and lukF-PV gene sequences. The prevalence of PVL+MRSA was 19.04%. They belonged to different genetic clones with multi-locus sequence types (MLST) 30, 80, and the novel type 1010. ST80 strains showed unique antibiotic resistance profile that was distinguishable from the European ST80 clone: they had no resistance to tetracycline and fusidic acid. Two nonsynonymous substitutions in the lukS-PV gene region and two haplotype variants among these isolates were detected. We revealed that PVL is a good marker for CA-MRSA infections and that PVL+ isolates belonged to different genotypes. SN - 1618-0623 UR - https://www.unboundmedicine.com/medline/citation/19616420/Molecular_characterization_of_Panton_Valentine_leukocidin_positive_community_acquired_methicillin_resistant_Staphylococcus_aureus_isolates_in_Egypt_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-5013(09)00021-4 DB - PRIME DP - Unbound Medicine ER -