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Bacteria in the gut of Japanese honeybee, Apis cerana japonica, and their antagonistic effect against Paenibacillus larvae, the causal agent of American foulbrood.
J Invertebr Pathol. 2009 Oct; 102(2):91-6.JI

Abstract

We assessed the complexity of bacterial communities occurring in the digestive tract of the Japanese honeybee, Apis cerana japonica, using histological and 16S rRNA gene sequence analyzes. Both Gram-positive and -negative bacteria were observed, and the number of gut bacteria was higher in old larvae compared with young larvae. A total of 35 clones were obtained by a culture-dependent method, and 16S rRNA gene sequence analysis revealed that the bacterial population in the gut of Japanese honeybee was diverse, including the phyla firmicutes, actinobacteria, and alpha-, beta-, and gammaproteobacteria. Further investigation by in vitro inhibition assays was carried out to determine the ability of an isolate to inhibit Paenibacillus larvae, the causal agent of American foulbrood. Out of 35 isolates, seven showed strong inhibitory activity against P. larvae. Most of the antagonistic bacteria belonged to Bacillus species, suggesting that the bacterial isolates obtained in this study appear to be potential candidates for the biological control of P. larvae.

Authors+Show Affiliations

Honeybee Research Group, Animal Breeding and Reproduction Research Team, National Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan. yoshiyam@affrc.go.jpNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19616552

Citation

Yoshiyama, Mikio, and Kiyoshi Kimura. "Bacteria in the Gut of Japanese Honeybee, Apis Cerana Japonica, and Their Antagonistic Effect Against Paenibacillus Larvae, the Causal Agent of American Foulbrood." Journal of Invertebrate Pathology, vol. 102, no. 2, 2009, pp. 91-6.
Yoshiyama M, Kimura K. Bacteria in the gut of Japanese honeybee, Apis cerana japonica, and their antagonistic effect against Paenibacillus larvae, the causal agent of American foulbrood. J Invertebr Pathol. 2009;102(2):91-6.
Yoshiyama, M., & Kimura, K. (2009). Bacteria in the gut of Japanese honeybee, Apis cerana japonica, and their antagonistic effect against Paenibacillus larvae, the causal agent of American foulbrood. Journal of Invertebrate Pathology, 102(2), 91-6. https://doi.org/10.1016/j.jip.2009.07.005
Yoshiyama M, Kimura K. Bacteria in the Gut of Japanese Honeybee, Apis Cerana Japonica, and Their Antagonistic Effect Against Paenibacillus Larvae, the Causal Agent of American Foulbrood. J Invertebr Pathol. 2009;102(2):91-6. PubMed PMID: 19616552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacteria in the gut of Japanese honeybee, Apis cerana japonica, and their antagonistic effect against Paenibacillus larvae, the causal agent of American foulbrood. AU - Yoshiyama,Mikio, AU - Kimura,Kiyoshi, Y1 - 2009/07/17/ PY - 2009/06/04/received PY - 2009/07/08/revised PY - 2009/07/13/accepted PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2009/12/16/medline SP - 91 EP - 6 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 102 IS - 2 N2 - We assessed the complexity of bacterial communities occurring in the digestive tract of the Japanese honeybee, Apis cerana japonica, using histological and 16S rRNA gene sequence analyzes. Both Gram-positive and -negative bacteria were observed, and the number of gut bacteria was higher in old larvae compared with young larvae. A total of 35 clones were obtained by a culture-dependent method, and 16S rRNA gene sequence analysis revealed that the bacterial population in the gut of Japanese honeybee was diverse, including the phyla firmicutes, actinobacteria, and alpha-, beta-, and gammaproteobacteria. Further investigation by in vitro inhibition assays was carried out to determine the ability of an isolate to inhibit Paenibacillus larvae, the causal agent of American foulbrood. Out of 35 isolates, seven showed strong inhibitory activity against P. larvae. Most of the antagonistic bacteria belonged to Bacillus species, suggesting that the bacterial isolates obtained in this study appear to be potential candidates for the biological control of P. larvae. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/19616552/Bacteria_in_the_gut_of_Japanese_honeybee_Apis_cerana_japonica_and_their_antagonistic_effect_against_Paenibacillus_larvae_the_causal_agent_of_American_foulbrood_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(09)00144-X DB - PRIME DP - Unbound Medicine ER -