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Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts.
J Nutr Biochem 2010; 21(7):659-64JN

Abstract

CXC chemokine ligand 10 (CXCL10) plays a pivotal role in the recruitment of Th1 cells and, thus, in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins derived from green tea, have multiple beneficial effects, but the effects of catechins on CXCL10 production from human gingival fibroblasts (HGFs) is not known. In this study, we investigated the mechanisms by which EGCG and ECG inhibit oncostatin M (OSM)-induced CXCL10 production in HGFs. HGFs constitutively expressed glycoprotein 130 and OSM receptor beta (OSMR beta), which are OSM receptors. OSM increased CXCL10 production in a concentration-dependent manner. EGCG and ECG prevented OSM-mediated CXCL10 production by HGFs. Inhibitors of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase (JNK), phosphatidylinositol-3-OH kinase and signal transducer and activator of transcription (STAT)3 decreased OSM-induced CXCL10 production. EGCG significantly prevented OSM-induced phosphorylation of JNK, Akt (Ser473) and STAT3 (Tyr705 and Ser727). ECG prevented phosphorylation of JNK and Akt (Ser473). In addition, EGCG and ECG attenuated OSMR beta expression on HGFs. These data provide a novel mechanism through which the green tea flavonoids, catechins, can provide direct benefits in periodontal disease.

Authors+Show Affiliations

Department of Conservative Dentistry and Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Tokushima 770-8504, Japan. hosokawa@dent.tokushima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19616927

Citation

Hosokawa, Yoshitaka, et al. "Catechins Inhibit CXCL10 Production From Oncostatin M-stimulated Human Gingival Fibroblasts." The Journal of Nutritional Biochemistry, vol. 21, no. 7, 2010, pp. 659-64.
Hosokawa Y, Hosokawa I, Ozaki K, et al. Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts. J Nutr Biochem. 2010;21(7):659-64.
Hosokawa, Y., Hosokawa, I., Ozaki, K., Nakanishi, T., Nakae, H., & Matsuo, T. (2010). Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts. The Journal of Nutritional Biochemistry, 21(7), pp. 659-64. doi:10.1016/j.jnutbio.2009.04.005.
Hosokawa Y, et al. Catechins Inhibit CXCL10 Production From Oncostatin M-stimulated Human Gingival Fibroblasts. J Nutr Biochem. 2010;21(7):659-64. PubMed PMID: 19616927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts. AU - Hosokawa,Yoshitaka, AU - Hosokawa,Ikuko, AU - Ozaki,Kazumi, AU - Nakanishi,Tadashi, AU - Nakae,Hideaki, AU - Matsuo,Takashi, Y1 - 2009/07/18/ PY - 2008/11/21/received PY - 2009/04/06/revised PY - 2009/04/13/accepted PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2010/10/6/medline SP - 659 EP - 64 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 21 IS - 7 N2 - CXC chemokine ligand 10 (CXCL10) plays a pivotal role in the recruitment of Th1 cells and, thus, in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins derived from green tea, have multiple beneficial effects, but the effects of catechins on CXCL10 production from human gingival fibroblasts (HGFs) is not known. In this study, we investigated the mechanisms by which EGCG and ECG inhibit oncostatin M (OSM)-induced CXCL10 production in HGFs. HGFs constitutively expressed glycoprotein 130 and OSM receptor beta (OSMR beta), which are OSM receptors. OSM increased CXCL10 production in a concentration-dependent manner. EGCG and ECG prevented OSM-mediated CXCL10 production by HGFs. Inhibitors of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase (JNK), phosphatidylinositol-3-OH kinase and signal transducer and activator of transcription (STAT)3 decreased OSM-induced CXCL10 production. EGCG significantly prevented OSM-induced phosphorylation of JNK, Akt (Ser473) and STAT3 (Tyr705 and Ser727). ECG prevented phosphorylation of JNK and Akt (Ser473). In addition, EGCG and ECG attenuated OSMR beta expression on HGFs. These data provide a novel mechanism through which the green tea flavonoids, catechins, can provide direct benefits in periodontal disease. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/19616927/Catechins_inhibit_CXCL10_production_from_oncostatin_M_stimulated_human_gingival_fibroblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(09)00089-8 DB - PRIME DP - Unbound Medicine ER -