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Genetic variability in sunflower (Helianthus annuus L.) and in the Helianthus genus as assessed by retrotransposon-based molecular markers.
Theor Appl Genet. 2009 Oct; 119(6):1027-38.TA

Abstract

The inter-retrotransposon amplified polymorphism (IRAP) protocol was applied for the first time within the genus Helianthus to assess intraspecific variability based on retrotransposon sequences among 36 wild accessions and 26 cultivars of Helianthus annuus L., and interspecific variability among 39 species of Helianthus. Two groups of LTRs, one belonging to a Copia-like retroelement and the other to a putative retrotransposon of unknown nature (SURE) have been isolated, sequenced and primers were designed to obtain IRAP fingerprints. The number of polymorphic bands in H. annuus wild accessions is as high as in Helianthus species. If we assume that a polymorphic band can be related to a retrotransposon insertion, this result suggests that retrotransposon activity continued after Helianthus speciation. Calculation of similarity indices from binary matrices (Shannon's and Jaccard's indices) show that variability is reduced among domesticated H. annuus. On the contrary, similarity indices among Helianthus species were as large as those observed among wild H. annuus accessions, probably related to their scattered geographic distribution. Principal component analysis of IRAP fingerprints allows the distinction between perennial and annual Helianthus species especially when the SURE element is concerned.

Authors+Show Affiliations

Department of Crop Plant Biology, Genetics Section, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19618160

Citation

Vukich, M, et al. "Genetic Variability in Sunflower (Helianthus Annuus L.) and in the Helianthus Genus as Assessed By Retrotransposon-based Molecular Markers." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 119, no. 6, 2009, pp. 1027-38.
Vukich M, Schulman AH, Giordani T, et al. Genetic variability in sunflower (Helianthus annuus L.) and in the Helianthus genus as assessed by retrotransposon-based molecular markers. Theor Appl Genet. 2009;119(6):1027-38.
Vukich, M., Schulman, A. H., Giordani, T., Natali, L., Kalendar, R., & Cavallini, A. (2009). Genetic variability in sunflower (Helianthus annuus L.) and in the Helianthus genus as assessed by retrotransposon-based molecular markers. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 119(6), 1027-38. https://doi.org/10.1007/s00122-009-1106-2
Vukich M, et al. Genetic Variability in Sunflower (Helianthus Annuus L.) and in the Helianthus Genus as Assessed By Retrotransposon-based Molecular Markers. Theor Appl Genet. 2009;119(6):1027-38. PubMed PMID: 19618160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic variability in sunflower (Helianthus annuus L.) and in the Helianthus genus as assessed by retrotransposon-based molecular markers. AU - Vukich,M, AU - Schulman,A H, AU - Giordani,T, AU - Natali,L, AU - Kalendar,R, AU - Cavallini,A, Y1 - 2009/07/19/ PY - 2009/03/07/received PY - 2009/06/30/accepted PY - 2009/7/21/entrez PY - 2009/7/21/pubmed PY - 2009/12/16/medline SP - 1027 EP - 38 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 119 IS - 6 N2 - The inter-retrotransposon amplified polymorphism (IRAP) protocol was applied for the first time within the genus Helianthus to assess intraspecific variability based on retrotransposon sequences among 36 wild accessions and 26 cultivars of Helianthus annuus L., and interspecific variability among 39 species of Helianthus. Two groups of LTRs, one belonging to a Copia-like retroelement and the other to a putative retrotransposon of unknown nature (SURE) have been isolated, sequenced and primers were designed to obtain IRAP fingerprints. The number of polymorphic bands in H. annuus wild accessions is as high as in Helianthus species. If we assume that a polymorphic band can be related to a retrotransposon insertion, this result suggests that retrotransposon activity continued after Helianthus speciation. Calculation of similarity indices from binary matrices (Shannon's and Jaccard's indices) show that variability is reduced among domesticated H. annuus. On the contrary, similarity indices among Helianthus species were as large as those observed among wild H. annuus accessions, probably related to their scattered geographic distribution. Principal component analysis of IRAP fingerprints allows the distinction between perennial and annual Helianthus species especially when the SURE element is concerned. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/19618160/Genetic_variability_in_sunflower__Helianthus_annuus_L___and_in_the_Helianthus_genus_as_assessed_by_retrotransposon_based_molecular_markers_ L2 - https://dx.doi.org/10.1007/s00122-009-1106-2 DB - PRIME DP - Unbound Medicine ER -