Tags

Type your tag names separated by a space and hit enter

[HPLC fingerprint of chemical constituents of Flos Farfarae].
Yao Xue Xue Bao. 2009 May; 44(5):510-4.YX

Abstract

This paper is aimed to establish the method of fingerprint analysis of chemical constituents by reversed-phase high-performance liquid chromatographic (HPLC) with diode array detector (DAD) for the quality control of the flower buds of Tussilago farfara L. (Flos Farfarae). The method was performed on a Dikma Diamonsil C18 column (250 mm x 4.6 mm ID, 5 gim) with a mixed mobile phase of 0.03% trifluoroacetic acid solution and acetonitrile in a gradient mode. The flow rate was 1.0 mL x min(-1) and the wavelength of measurement was 240 nm. Ten batches of the Flos Farfarae were determined. The HPLC chromatographic fingerprint of chemical constituents was established from the 10 batches of the Flos Farfarae and showed 25 characteristic common peaks, among which 16 peaks were recognized and 18 compounds (adenosine, uridine, gallic acid, 3-O-caffeoylquinic acid, p-hydroxybenzoic acid, trans-caffeic acid, phthalic acid, rutin, hyperoside, 3,5-O-dicaffeoylquinic acid, kaempferol-3-O-beta-D-glucopyranoside, isoferulic acid, ferulic acid, quercetin, 2,2-dimethyl-6-acetyl chromanone, dibutylphthalate, tussilagone, 7beta-(3'-ethylcrotonoyloxy)-1alpha-(2'-methyl-butyryloxy)-3,14-dehydro-E-notonipetranone) were determined by comparison with chromatographic behaviors and UV spectra of the authentic compounds. The 10 batches of samples were classified as 2 clusters by cluster analysis and 6 samples were confirmed to establish the mutual model. The samples' quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 B version). The convenient and high specific method can be used to identify and evaluate the quality of the Flos Farfarae.

Authors+Show Affiliations

Liu YF
State Key Laboratory of Natural and Biomimetic Drugs, Peking University, China.
Yang XW
No affiliation info available

MeSH

Chemistry, PharmaceuticalChromatography, High Pressure LiquidCluster AnalysisDrugs, Chinese HerbalFlowersPlants, MedicinalQuality ControlTussilago

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19618728

Citation

Liu, Yu-feng, and Xiu-wei Yang. "[HPLC Fingerprint of Chemical Constituents of Flos Farfarae]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 44, no. 5, 2009, pp. 510-4.
Liu YF, Yang XW. [HPLC fingerprint of chemical constituents of Flos Farfarae]. Yao Xue Xue Bao. 2009;44(5):510-4.
Liu, Y. F., & Yang, X. W. (2009). [HPLC fingerprint of chemical constituents of Flos Farfarae]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 44(5), 510-4.
Liu YF, Yang XW. [HPLC Fingerprint of Chemical Constituents of Flos Farfarae]. Yao Xue Xue Bao. 2009;44(5):510-4. PubMed PMID: 19618728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [HPLC fingerprint of chemical constituents of Flos Farfarae]. AU - Liu,Yu-feng, AU - Yang,Xiu-wei, PY - 2009/7/22/entrez PY - 2009/7/22/pubmed PY - 2010/4/7/medline SP - 510 EP - 4 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 44 IS - 5 N2 - This paper is aimed to establish the method of fingerprint analysis of chemical constituents by reversed-phase high-performance liquid chromatographic (HPLC) with diode array detector (DAD) for the quality control of the flower buds of Tussilago farfara L. (Flos Farfarae). The method was performed on a Dikma Diamonsil C18 column (250 mm x 4.6 mm ID, 5 gim) with a mixed mobile phase of 0.03% trifluoroacetic acid solution and acetonitrile in a gradient mode. The flow rate was 1.0 mL x min(-1) and the wavelength of measurement was 240 nm. Ten batches of the Flos Farfarae were determined. The HPLC chromatographic fingerprint of chemical constituents was established from the 10 batches of the Flos Farfarae and showed 25 characteristic common peaks, among which 16 peaks were recognized and 18 compounds (adenosine, uridine, gallic acid, 3-O-caffeoylquinic acid, p-hydroxybenzoic acid, trans-caffeic acid, phthalic acid, rutin, hyperoside, 3,5-O-dicaffeoylquinic acid, kaempferol-3-O-beta-D-glucopyranoside, isoferulic acid, ferulic acid, quercetin, 2,2-dimethyl-6-acetyl chromanone, dibutylphthalate, tussilagone, 7beta-(3'-ethylcrotonoyloxy)-1alpha-(2'-methyl-butyryloxy)-3,14-dehydro-E-notonipetranone) were determined by comparison with chromatographic behaviors and UV spectra of the authentic compounds. The 10 batches of samples were classified as 2 clusters by cluster analysis and 6 samples were confirmed to establish the mutual model. The samples' quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 B version). The convenient and high specific method can be used to identify and evaluate the quality of the Flos Farfarae. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/19618728/[HPLC_fingerprint_of_chemical_constituents_of_Flos_Farfarae]_ DB - PRIME DP - Unbound Medicine ER -