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Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder.
Neurosci Res. 2009 Nov; 65(3):245-51.NR

Abstract

The neurochemical phenotypes of the transient receptor potential melastatin-8 (TRPM8)-immunoreactive afferent neurons innervating the rat urinary bladder were examined by using a highly sensitive tyramide signal amplification method, combined with wheat-germ agglutinin-horseradish peroxidase (WGA-HRP) retrograde tracing. TRPM8-immunoreactivity was detected in a small proportion of the WGA-HRP-labeled bladder afferent neurons in the dorsal root ganglia of the Th13-L1 (1.14%) and the L6-S1 (1.27%), and these neurons were small in size (<600 microm(2)). The 82.6+/-3.8% of the TRPM8-immunoreactive bladder afferent neurons and 80.9+/-1.5% of the total population of the TRPM8-immunoreactive afferent neurons in the observed dorsal root ganglia expressed NF200. On the other hand, the proportions of the co-expression of TRPM8 and nociceptive markers such as calcitonin gene-related peptide (CGRP), transient receptor potential vanilloid-1 (TRPV1), and isolectin B4 (IB4) in the bladder afferent neurons (81.5+/-5.2% for CGRP, 36.1+/-4.0% for TRPV1, and 15.8+/-5.5% for IB4) were higher in comparison to those in the total population of the TRPM8-immunoreactive afferent neurons (21.9+/-2.4% for CGRP, 16.6+/-1.7% for TRPV1, and 5.4+/-0.5% for IB4), although no significant difference existed for IB4. Our results suggest that the TRPM8-expressing bladder afferents should be classified as Adelta-fibers and C-fibers, while some of these afferents may be involved in nociceptive sensations.

Authors+Show Affiliations

Department of Urology, Kurume University School of Medicine, Asahimachi 67, Kurume 830-0011, Fukuoka, Japan. hayashi_tokumasa@kurume-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19622375

Citation

Hayashi, Tokumasa, et al. "Expression of the TRPM8-immunoreactivity in Dorsal Root Ganglion Neurons Innervating the Rat Urinary Bladder." Neuroscience Research, vol. 65, no. 3, 2009, pp. 245-51.
Hayashi T, Kondo T, Ishimatsu M, et al. Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder. Neurosci Res. 2009;65(3):245-51.
Hayashi, T., Kondo, T., Ishimatsu, M., Yamada, S., Nakamura, K., Matsuoka, K., & Akasu, T. (2009). Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder. Neuroscience Research, 65(3), 245-51. https://doi.org/10.1016/j.neures.2009.07.005
Hayashi T, et al. Expression of the TRPM8-immunoreactivity in Dorsal Root Ganglion Neurons Innervating the Rat Urinary Bladder. Neurosci Res. 2009;65(3):245-51. PubMed PMID: 19622375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder. AU - Hayashi,Tokumasa, AU - Kondo,Teruyoshi, AU - Ishimatsu,Masaru, AU - Yamada,Satoko, AU - Nakamura,Kei-ichiro, AU - Matsuoka,Kei, AU - Akasu,Takashi, Y1 - 2009/07/19/ PY - 2009/04/16/received PY - 2009/07/06/revised PY - 2009/07/14/accepted PY - 2009/7/23/entrez PY - 2009/7/23/pubmed PY - 2010/1/19/medline SP - 245 EP - 51 JF - Neuroscience research JO - Neurosci Res VL - 65 IS - 3 N2 - The neurochemical phenotypes of the transient receptor potential melastatin-8 (TRPM8)-immunoreactive afferent neurons innervating the rat urinary bladder were examined by using a highly sensitive tyramide signal amplification method, combined with wheat-germ agglutinin-horseradish peroxidase (WGA-HRP) retrograde tracing. TRPM8-immunoreactivity was detected in a small proportion of the WGA-HRP-labeled bladder afferent neurons in the dorsal root ganglia of the Th13-L1 (1.14%) and the L6-S1 (1.27%), and these neurons were small in size (<600 microm(2)). The 82.6+/-3.8% of the TRPM8-immunoreactive bladder afferent neurons and 80.9+/-1.5% of the total population of the TRPM8-immunoreactive afferent neurons in the observed dorsal root ganglia expressed NF200. On the other hand, the proportions of the co-expression of TRPM8 and nociceptive markers such as calcitonin gene-related peptide (CGRP), transient receptor potential vanilloid-1 (TRPV1), and isolectin B4 (IB4) in the bladder afferent neurons (81.5+/-5.2% for CGRP, 36.1+/-4.0% for TRPV1, and 15.8+/-5.5% for IB4) were higher in comparison to those in the total population of the TRPM8-immunoreactive afferent neurons (21.9+/-2.4% for CGRP, 16.6+/-1.7% for TRPV1, and 5.4+/-0.5% for IB4), although no significant difference existed for IB4. Our results suggest that the TRPM8-expressing bladder afferents should be classified as Adelta-fibers and C-fibers, while some of these afferents may be involved in nociceptive sensations. SN - 1872-8111 UR - https://www.unboundmedicine.com/medline/citation/19622375/Expression_of_the_TRPM8_immunoreactivity_in_dorsal_root_ganglion_neurons_innervating_the_rat_urinary_bladder_ DB - PRIME DP - Unbound Medicine ER -