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[Study on HPLC fingerprint of Trachelospermum jasminoides].
Zhongguo Zhong Yao Za Zhi. 2009 Mar; 34(6):727-30.ZZ

Abstract

OBJECTIVE

To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides.

METHOD

The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column (4.6 mm x 150 mm, 5 microm) with H2O (A) and methanol (B) as mobile phases in gradient mode. The elutin conditions were 0-15 min, changed from 10% B to 30% B; 15-40 min, to 40% B; 40-60 min, to 60% B. The column temperature was set at 30 degrees C and the flow rate was 0.8 mL x min(-1) with the detection wavelength of 230 nm.

RESULT

The HPLC chromatographic fingerprint of T. jasminoides, showing 19 characteristic peaks, was established from 14 lots of T. jasminoides. The similarity of every lot of T. jasminoides was calculated as good in general, the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity.

CONCLUSION

The chromatographic fingerprint of T. jasminoides with high characteristics and specificity can be used to control its quality.

Authors+Show Affiliations

Liu Y
School of Pharmaceutical and Technology, Tianjin University, Tianjin 300072, China.
Yu N
No affiliation info available
Yang X
No affiliation info available
Zhao Y
No affiliation info available

MeSH

ApocynaceaeChromatography, High Pressure LiquidDrugs, Chinese HerbalQuality Control

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19624016

Citation

Liu, Yiquan, et al. "[Study On HPLC Fingerprint of Trachelospermum Jasminoides]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 34, no. 6, 2009, pp. 727-30.
Liu Y, Yu N, Yang X, et al. [Study on HPLC fingerprint of Trachelospermum jasminoides]. Zhongguo Zhong Yao Za Zhi. 2009;34(6):727-30.
Liu, Y., Yu, N., Yang, X., & Zhao, Y. (2009). [Study on HPLC fingerprint of Trachelospermum jasminoides]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 34(6), 727-30.
Liu Y, et al. [Study On HPLC Fingerprint of Trachelospermum Jasminoides]. Zhongguo Zhong Yao Za Zhi. 2009;34(6):727-30. PubMed PMID: 19624016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on HPLC fingerprint of Trachelospermum jasminoides]. AU - Liu,Yiquan, AU - Yu,Nengjiang, AU - Yang,Xuedong, AU - Zhao,Yimin, PY - 2009/7/24/entrez PY - 2009/7/25/pubmed PY - 2009/9/2/medline SP - 727 EP - 30 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 34 IS - 6 N2 - OBJECTIVE: To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides. METHOD: The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column (4.6 mm x 150 mm, 5 microm) with H2O (A) and methanol (B) as mobile phases in gradient mode. The elutin conditions were 0-15 min, changed from 10% B to 30% B; 15-40 min, to 40% B; 40-60 min, to 60% B. The column temperature was set at 30 degrees C and the flow rate was 0.8 mL x min(-1) with the detection wavelength of 230 nm. RESULT: The HPLC chromatographic fingerprint of T. jasminoides, showing 19 characteristic peaks, was established from 14 lots of T. jasminoides. The similarity of every lot of T. jasminoides was calculated as good in general, the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity. CONCLUSION: The chromatographic fingerprint of T. jasminoides with high characteristics and specificity can be used to control its quality. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/19624016/[Study_on_HPLC_fingerprint_of_Trachelospermum_jasminoides]_ DB - PRIME DP - Unbound Medicine ER -