Tags

Type your tag names separated by a space and hit enter

The non-specific lipid transfer protein, Ara h 9, is an important allergen in peanut.
Clin Exp Allergy. 2009 Sep; 39(9):1427-37.CE

Abstract

BACKGROUND

Plant food allergy in the Mediterranean area is mainly caused by non-specific lipid transfer proteins (nsLTP). The aim of this study was to characterize peanut nsLTP in comparison with peach nsLTP, Pru p 3, and assess its importance in peanut allergy.

METHODS

Peanut-allergic patients from Spain (n=32) were included on the basis of a positive case history and either a positive skin prick test or specific IgE to peanut. For comparison, sera of 41 peanut-allergic subjects from outside the Mediterranean area were used. Natural Ara h 9 and two isoforms of recombinant Ara h 9, expressed in Pichia pastoris, were purified using a two-step chromatographic procedure. Allergen characterization was carried out by N-terminal sequencing, circular dichroism (CD) spectroscopy, immunoblotting, IgE inhibition tests and basophil histamine release assays.

RESULTS

Compared with natural peanut nsLTP, the recombinant proteins could be purified in high amounts from yeast supernatant (> or =45 mg/L). The identity of the proteins was verified by N-terminal amino acid sequencing and with rabbit nsLTP-specific antibodies. CD spectroscopy revealed similar secondary structures for all preparations and Pru p 3. The Ara h 9 isoforms showed 62-68% amino acid sequence identity with Pru p 3. IgE antibody reactivity to rAra h 9 was present in 29/32 Spanish and 6/41 non-Mediterranean subjects. Recombinant Ara h 9 showed strong cross-reactivity to nPru p 3 and similar IgE-binding capacity as nAra h 9. The two Ara h 9 isoforms displayed similar IgE reactivity. In peanut-allergic patients with concomitant peach allergy, Ara h 9 showed a weaker allergenic potency than Pru p 3 in histamine release assays.

CONCLUSIONS

Ara h 9 is a major allergen in peanut-allergic patients from the Mediterranean area. Ara h 9 is capable of inducing histamine release from basophils, but to a lesser extent than Pru p 3.

Authors+Show Affiliations

Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany. lauir@pei.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19624524

Citation

Lauer, I, et al. "The Non-specific Lipid Transfer Protein, Ara H 9, Is an Important Allergen in Peanut." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 39, no. 9, 2009, pp. 1427-37.
Lauer I, Dueringer N, Pokoj S, et al. The non-specific lipid transfer protein, Ara h 9, is an important allergen in peanut. Clin Exp Allergy. 2009;39(9):1427-37.
Lauer, I., Dueringer, N., Pokoj, S., Rehm, S., Zoccatelli, G., Reese, G., Miguel-Moncin, M. S., Cistero-Bahima, A., Enrique, E., Lidholm, J., Vieths, S., & Scheurer, S. (2009). The non-specific lipid transfer protein, Ara h 9, is an important allergen in peanut. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 39(9), 1427-37. https://doi.org/10.1111/j.1365-2222.2009.03312.x
Lauer I, et al. The Non-specific Lipid Transfer Protein, Ara H 9, Is an Important Allergen in Peanut. Clin Exp Allergy. 2009;39(9):1427-37. PubMed PMID: 19624524.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The non-specific lipid transfer protein, Ara h 9, is an important allergen in peanut. AU - Lauer,I, AU - Dueringer,N, AU - Pokoj,S, AU - Rehm,S, AU - Zoccatelli,G, AU - Reese,G, AU - Miguel-Moncin,M S, AU - Cistero-Bahima,A, AU - Enrique,E, AU - Lidholm,J, AU - Vieths,S, AU - Scheurer,S, Y1 - 2009/07/16/ PY - 2009/7/24/entrez PY - 2009/7/25/pubmed PY - 2009/11/18/medline SP - 1427 EP - 37 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 39 IS - 9 N2 - BACKGROUND: Plant food allergy in the Mediterranean area is mainly caused by non-specific lipid transfer proteins (nsLTP). The aim of this study was to characterize peanut nsLTP in comparison with peach nsLTP, Pru p 3, and assess its importance in peanut allergy. METHODS: Peanut-allergic patients from Spain (n=32) were included on the basis of a positive case history and either a positive skin prick test or specific IgE to peanut. For comparison, sera of 41 peanut-allergic subjects from outside the Mediterranean area were used. Natural Ara h 9 and two isoforms of recombinant Ara h 9, expressed in Pichia pastoris, were purified using a two-step chromatographic procedure. Allergen characterization was carried out by N-terminal sequencing, circular dichroism (CD) spectroscopy, immunoblotting, IgE inhibition tests and basophil histamine release assays. RESULTS: Compared with natural peanut nsLTP, the recombinant proteins could be purified in high amounts from yeast supernatant (> or =45 mg/L). The identity of the proteins was verified by N-terminal amino acid sequencing and with rabbit nsLTP-specific antibodies. CD spectroscopy revealed similar secondary structures for all preparations and Pru p 3. The Ara h 9 isoforms showed 62-68% amino acid sequence identity with Pru p 3. IgE antibody reactivity to rAra h 9 was present in 29/32 Spanish and 6/41 non-Mediterranean subjects. Recombinant Ara h 9 showed strong cross-reactivity to nPru p 3 and similar IgE-binding capacity as nAra h 9. The two Ara h 9 isoforms displayed similar IgE reactivity. In peanut-allergic patients with concomitant peach allergy, Ara h 9 showed a weaker allergenic potency than Pru p 3 in histamine release assays. CONCLUSIONS: Ara h 9 is a major allergen in peanut-allergic patients from the Mediterranean area. Ara h 9 is capable of inducing histamine release from basophils, but to a lesser extent than Pru p 3. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/19624524/The_non_specific_lipid_transfer_protein_Ara_h_9_is_an_important_allergen_in_peanut_ L2 - https://doi.org/10.1111/j.1365-2222.2009.03312.x DB - PRIME DP - Unbound Medicine ER -