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Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trial.
Nutr J 2009; 8:34NJ

Abstract

BACKGROUND

Whey protein is a potential source of bioactive peptides. Based on findings from in vitro experiments indicating a novel whey derived peptide (NOP-47) increased endothelial nitric oxide synthesis, we tested its effects on vascular function in humans.

METHODS

A randomized, placebo-controlled, crossover study design was used. Healthy men (n = 10) and women (n = 10) (25 +/- 5 y, BMI = 24.3 +/- 2.3 kg/m2) participated in two vascular testing days each preceded by 2 wk of supplementation with a single dose of 5 g/day of a novel whey-derived peptide (NOP-47) or placebo. There was a 2 wk washout period between trials. After 2 wk of supplementation, vascular function in the forearm and circulating oxidative stress and inflammatory related biomarkers were measured serially for 2 h after ingestion of 5 g of NOP-47 or placebo. Macrovascular and microvascular function were assessed using brachial artery flow mediated dilation (FMD) and venous occlusion strain gauge plethysmography.

RESULTS

Baseline peak FMD was not different for Placebo (7.7%) and NOP-47 (7.8%). Placebo had no effect on FMD at 30, 60, and 90 min post-ingestion (7.5%, 7.2%, and 7.6%, respectively) whereas NOP-47 significantly improved FMD responses at these respective postprandial time points compared to baseline (8.9%, 9.9%, and 9.0%; P < 0.0001 for time x trial interaction). Baseline reactive hyperemia forearm blood flow was not different for placebo (27.2 +/- 7.2%/min) and NOP-47 (27.3 +/- 7.6%/min). Hyperemia blood flow measured 120 min post-ingestion (27.2 +/- 7.8%/min) was unaffected by placebo whereas NOP-47 significantly increased hyperemia compared to baseline (29.9 +/- 7.8%/min; P = 0.008 for time x trial interaction). Plasma myeloperoxidase was increased transiently by both NOP-47 and placebo, but there were no changes in markers inflammation. Plasma total nitrites/nitrates significantly decreased over the 2 hr post-ingestion period and were lower at 120 min after placebo (-25%) compared to NOP-47 (-18%).

CONCLUSION

These findings indicate that supplementation with a novel whey-derived peptide in healthy individuals improves vascular function.

Authors+Show Affiliations

Department of Kinesiology, University of Connecticut, 2095 Hillside Road, Unit 1110, Storrs, CT 06269, USA. kevin.ballard@uconn.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

19624856

Citation

Ballard, Kevin D., et al. "Acute Ingestion of a Novel Whey-derived Peptide Improves Vascular Endothelial Responses in Healthy Individuals: a Randomized, Placebo Controlled Trial." Nutrition Journal, vol. 8, 2009, p. 34.
Ballard KD, Bruno RS, Seip RL, et al. Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trial. Nutr J. 2009;8:34.
Ballard, K. D., Bruno, R. S., Seip, R. L., Quann, E. E., Volk, B. M., Freidenreich, D. J., ... Volek, J. S. (2009). Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trial. Nutrition Journal, 8, p. 34. doi:10.1186/1475-2891-8-34.
Ballard KD, et al. Acute Ingestion of a Novel Whey-derived Peptide Improves Vascular Endothelial Responses in Healthy Individuals: a Randomized, Placebo Controlled Trial. Nutr J. 2009 Jul 22;8:34. PubMed PMID: 19624856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trial. AU - Ballard,Kevin D, AU - Bruno,Richard S, AU - Seip,Richard L, AU - Quann,Erin E, AU - Volk,Brittanie M, AU - Freidenreich,Daniel J, AU - Kawiecki,Diana M, AU - Kupchak,Brian R, AU - Chung,Min-Yu, AU - Kraemer,William J, AU - Volek,Jeff S, Y1 - 2009/07/22/ PY - 2009/03/12/received PY - 2009/07/22/accepted PY - 2009/7/24/entrez PY - 2009/7/25/pubmed PY - 2009/10/9/medline SP - 34 EP - 34 JF - Nutrition journal JO - Nutr J VL - 8 N2 - BACKGROUND: Whey protein is a potential source of bioactive peptides. Based on findings from in vitro experiments indicating a novel whey derived peptide (NOP-47) increased endothelial nitric oxide synthesis, we tested its effects on vascular function in humans. METHODS: A randomized, placebo-controlled, crossover study design was used. Healthy men (n = 10) and women (n = 10) (25 +/- 5 y, BMI = 24.3 +/- 2.3 kg/m2) participated in two vascular testing days each preceded by 2 wk of supplementation with a single dose of 5 g/day of a novel whey-derived peptide (NOP-47) or placebo. There was a 2 wk washout period between trials. After 2 wk of supplementation, vascular function in the forearm and circulating oxidative stress and inflammatory related biomarkers were measured serially for 2 h after ingestion of 5 g of NOP-47 or placebo. Macrovascular and microvascular function were assessed using brachial artery flow mediated dilation (FMD) and venous occlusion strain gauge plethysmography. RESULTS: Baseline peak FMD was not different for Placebo (7.7%) and NOP-47 (7.8%). Placebo had no effect on FMD at 30, 60, and 90 min post-ingestion (7.5%, 7.2%, and 7.6%, respectively) whereas NOP-47 significantly improved FMD responses at these respective postprandial time points compared to baseline (8.9%, 9.9%, and 9.0%; P < 0.0001 for time x trial interaction). Baseline reactive hyperemia forearm blood flow was not different for placebo (27.2 +/- 7.2%/min) and NOP-47 (27.3 +/- 7.6%/min). Hyperemia blood flow measured 120 min post-ingestion (27.2 +/- 7.8%/min) was unaffected by placebo whereas NOP-47 significantly increased hyperemia compared to baseline (29.9 +/- 7.8%/min; P = 0.008 for time x trial interaction). Plasma myeloperoxidase was increased transiently by both NOP-47 and placebo, but there were no changes in markers inflammation. Plasma total nitrites/nitrates significantly decreased over the 2 hr post-ingestion period and were lower at 120 min after placebo (-25%) compared to NOP-47 (-18%). CONCLUSION: These findings indicate that supplementation with a novel whey-derived peptide in healthy individuals improves vascular function. SN - 1475-2891 UR - https://www.unboundmedicine.com/medline/citation/19624856/Acute_ingestion_of_a_novel_whey_derived_peptide_improves_vascular_endothelial_responses_in_healthy_individuals:_a_randomized_placebo_controlled_trial_ L2 - https://nutritionj.biomedcentral.com/articles/10.1186/1475-2891-8-34 DB - PRIME DP - Unbound Medicine ER -