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Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus.
J Gen Virol. 2009 Nov; 90(Pt 11):2695-2703.JG

Abstract

Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4 % to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks.

Authors+Show Affiliations

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.AIDS Institute, The University of Hong Kong Li Ka Shing Faculty of Medicine, Hong Kong SAR.Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19625462

Citation

Xu, Lili, et al. "Angiotensin-converting Enzyme 2 (ACE2) From Raccoon Dog Can Serve as an Efficient Receptor for the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus." The Journal of General Virology, vol. 90, no. Pt 11, 2009, pp. 2695-2703.
Xu L, Zhang Y, Liu Y, et al. Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus. J Gen Virol. 2009;90(Pt 11):2695-2703.
Xu, L., Zhang, Y., Liu, Y., Chen, Z., Deng, H., Ma, Z., Wang, H., Hu, Z., & Deng, F. (2009). Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus. The Journal of General Virology, 90(Pt 11), 2695-2703. https://doi.org/10.1099/vir.0.013490-0
Xu L, et al. Angiotensin-converting Enzyme 2 (ACE2) From Raccoon Dog Can Serve as an Efficient Receptor for the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus. J Gen Virol. 2009;90(Pt 11):2695-2703. PubMed PMID: 19625462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus. AU - Xu,Lili, AU - Zhang,Yanfang, AU - Liu,Yun, AU - Chen,Zhiwei, AU - Deng,Hongkui, AU - Ma,Zhongbin, AU - Wang,Hualin, AU - Hu,Zhihong, AU - Deng,Fei, Y1 - 2009/07/22/ PY - 2009/7/24/entrez PY - 2009/7/25/pubmed PY - 2009/10/31/medline SP - 2695 EP - 2703 JF - The Journal of general virology JO - J Gen Virol VL - 90 IS - Pt 11 N2 - Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4 % to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks. SN - 1465-2099 UR - https://www.unboundmedicine.com/medline/citation/19625462/Angiotensin_converting_enzyme_2__ACE2__from_raccoon_dog_can_serve_as_an_efficient_receptor_for_the_spike_protein_of_severe_acute_respiratory_syndrome_coronavirus_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.013490-0 DB - PRIME DP - Unbound Medicine ER -