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Detection of four human coronaviruses in respiratory infections in children: a one-year study in Colorado.
J Med Virol. 2009 Sep; 81(9):1597-604.JM

Abstract

Lower respiratory tract infections are the leading cause of death in children worldwide. Studies on the epidemiology and clinical associations of the four human non-SARS human coronaviruses (HCoVs) using sensitive polymerase chain reaction (PCR) assays are needed to evaluate the clinical significance of HCoV infections worldwide. Pediatric respiratory specimens (1,683) submitted to a diagnostic virology laboratory over a 1-year period (December 2004-November 2005) that were negative for seven respiratory viruses by conventional methods were tested for RNA of four HCoVs using sensitive RT-PCR assays. Coronavirus RNAs were detected in 84 (5.0%) specimens: HCoV-NL63 in 37 specimens, HCoV-OC43 in 34, HCoV-229E in 11, and HCoV-HKU1 in 2. The majority of HCoV infections occurred during winter months, and over 62% were in previously healthy children. Twenty-six (41%) coronavirus positive patients had evidence of a lower respiratory tract infection (LRTI), 17 (26%) presented with vomiting and/or diarrhea, and 5 (8%) presented with meningoencephalitis or seizures. Respiratory specimens from one immunocompromised patient were persistently positive for HCoV-229E RNA for 3 months. HCoV-NL63-positive patients were nearly twice as likely to be hospitalized (P = 0.02) and to have a LRTI (P = 0.04) than HCoV-OC43-positive patients. HCoVs are associated with a small, but significant number (at least 2.4% of total samples submitted), of both upper and lower respiratory tract illnesses in children in Colorado. Our data raise the possibility that HCoV may play a role in gastrointestinal and CNS disease. Additional studies are needed to investigate the potential roles of HCoVs in these diseases.

Authors+Show Affiliations

Department of Pediatrics, The Children's Hospital, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA. samuel.dominguez@ucdenver.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19626607

Citation

Dominguez, Samuel R., et al. "Detection of Four Human Coronaviruses in Respiratory Infections in Children: a One-year Study in Colorado." Journal of Medical Virology, vol. 81, no. 9, 2009, pp. 1597-604.
Dominguez SR, Robinson CC, Holmes KV. Detection of four human coronaviruses in respiratory infections in children: a one-year study in Colorado. J Med Virol. 2009;81(9):1597-604.
Dominguez, S. R., Robinson, C. C., & Holmes, K. V. (2009). Detection of four human coronaviruses in respiratory infections in children: a one-year study in Colorado. Journal of Medical Virology, 81(9), 1597-604. https://doi.org/10.1002/jmv.21541
Dominguez SR, Robinson CC, Holmes KV. Detection of Four Human Coronaviruses in Respiratory Infections in Children: a One-year Study in Colorado. J Med Virol. 2009;81(9):1597-604. PubMed PMID: 19626607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of four human coronaviruses in respiratory infections in children: a one-year study in Colorado. AU - Dominguez,Samuel R, AU - Robinson,Christine C, AU - Holmes,Kathryn V, PY - 2009/7/24/entrez PY - 2009/7/25/pubmed PY - 2009/9/22/medline SP - 1597 EP - 604 JF - Journal of medical virology JO - J Med Virol VL - 81 IS - 9 N2 - Lower respiratory tract infections are the leading cause of death in children worldwide. Studies on the epidemiology and clinical associations of the four human non-SARS human coronaviruses (HCoVs) using sensitive polymerase chain reaction (PCR) assays are needed to evaluate the clinical significance of HCoV infections worldwide. Pediatric respiratory specimens (1,683) submitted to a diagnostic virology laboratory over a 1-year period (December 2004-November 2005) that were negative for seven respiratory viruses by conventional methods were tested for RNA of four HCoVs using sensitive RT-PCR assays. Coronavirus RNAs were detected in 84 (5.0%) specimens: HCoV-NL63 in 37 specimens, HCoV-OC43 in 34, HCoV-229E in 11, and HCoV-HKU1 in 2. The majority of HCoV infections occurred during winter months, and over 62% were in previously healthy children. Twenty-six (41%) coronavirus positive patients had evidence of a lower respiratory tract infection (LRTI), 17 (26%) presented with vomiting and/or diarrhea, and 5 (8%) presented with meningoencephalitis or seizures. Respiratory specimens from one immunocompromised patient were persistently positive for HCoV-229E RNA for 3 months. HCoV-NL63-positive patients were nearly twice as likely to be hospitalized (P = 0.02) and to have a LRTI (P = 0.04) than HCoV-OC43-positive patients. HCoVs are associated with a small, but significant number (at least 2.4% of total samples submitted), of both upper and lower respiratory tract illnesses in children in Colorado. Our data raise the possibility that HCoV may play a role in gastrointestinal and CNS disease. Additional studies are needed to investigate the potential roles of HCoVs in these diseases. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/19626607/Detection_of_four_human_coronaviruses_in_respiratory_infections_in_children:_a_one_year_study_in_Colorado_ L2 - https://doi.org/10.1002/jmv.21541 DB - PRIME DP - Unbound Medicine ER -