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Telomerase activity, telomere length and human telomerase reverse transcriptase expression in hepatocellular carcinoma is independent of hepatitis virus status.
Liver Int. 2009 Sep; 29(8):1162-70.LI

Abstract

BACKGROUND

Telomerase expression and the maintenance of a critical telomere length (TL) in cancer initiation indicates that telomere shortening and telomerase expression initiates cancer by induction of chromosomal instability.

METHODS

Telomerase activity, TL and human telomerase reverse transcriptase (hTERT) expression were investigated in 58 hepatocellular carcinoma (HCC) and 17 chronic hepatitis patients by the telomerase repeat amplification protocol, Southern blotting and reverse transcriptase-polymerase chain reaction.

RESULTS

Telomerase was positive in 76% of HCC and 11.8% of chronic hepatitis patients (P<0.0001). The mean telomere length (MTL) in HCC was significantly shorter compared with chronic hepatitis (P<0.0001). The MTL was not significantly different in HCC patients with and without cirrhosis (P=0.77). In hepatitis B virus, hepatitis C virus and non-B non-C-related HCC groups, no differences were found in telomerase activity and MTL (P=0.77). hTERT, a regulator of telomerase, was, however, positive in 81% of HCCs. The correlation between telomerase activity and hTERT mRNA expression was statistically significant (P<0.0001). The MTL in telomerase-positive HCC cases was significantly shorter than the MTL in telomerase-negative cases (P<0.0001).

CONCLUSION

The majority of HCCs exhibited telomerase activity that correlated well with hTERT expression. MTL in HCC was significantly shorter than chronic hepatitis. It was also found that shorter telomeres are present in telomerase-positive HCC cases. However, no correlation was found between telomerase activity and TL with respect to the viral status in HCC.

Authors+Show Affiliations

Department of Hepatology, Postgraduate Institute of Medical Education & Research, Chandigarh, India. nitin3112k@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19627485

Citation

Saini, Nitin, et al. "Telomerase Activity, Telomere Length and Human Telomerase Reverse Transcriptase Expression in Hepatocellular Carcinoma Is Independent of Hepatitis Virus Status." Liver International : Official Journal of the International Association for the Study of the Liver, vol. 29, no. 8, 2009, pp. 1162-70.
Saini N, Srinivasan R, Chawla Y, et al. Telomerase activity, telomere length and human telomerase reverse transcriptase expression in hepatocellular carcinoma is independent of hepatitis virus status. Liver Int. 2009;29(8):1162-70.
Saini, N., Srinivasan, R., Chawla, Y., Sharma, S., Chakraborti, A., & Rajwanshi, A. (2009). Telomerase activity, telomere length and human telomerase reverse transcriptase expression in hepatocellular carcinoma is independent of hepatitis virus status. Liver International : Official Journal of the International Association for the Study of the Liver, 29(8), 1162-70. https://doi.org/10.1111/j.1478-3231.2009.02082.x
Saini N, et al. Telomerase Activity, Telomere Length and Human Telomerase Reverse Transcriptase Expression in Hepatocellular Carcinoma Is Independent of Hepatitis Virus Status. Liver Int. 2009;29(8):1162-70. PubMed PMID: 19627485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase activity, telomere length and human telomerase reverse transcriptase expression in hepatocellular carcinoma is independent of hepatitis virus status. AU - Saini,Nitin, AU - Srinivasan,Radhika, AU - Chawla,Yogesh, AU - Sharma,Sanjeev, AU - Chakraborti,Anuradha, AU - Rajwanshi,Arvind, Y1 - 2009/07/17/ PY - 2009/7/25/entrez PY - 2009/7/25/pubmed PY - 2009/10/21/medline SP - 1162 EP - 70 JF - Liver international : official journal of the International Association for the Study of the Liver JO - Liver Int VL - 29 IS - 8 N2 - BACKGROUND: Telomerase expression and the maintenance of a critical telomere length (TL) in cancer initiation indicates that telomere shortening and telomerase expression initiates cancer by induction of chromosomal instability. METHODS: Telomerase activity, TL and human telomerase reverse transcriptase (hTERT) expression were investigated in 58 hepatocellular carcinoma (HCC) and 17 chronic hepatitis patients by the telomerase repeat amplification protocol, Southern blotting and reverse transcriptase-polymerase chain reaction. RESULTS: Telomerase was positive in 76% of HCC and 11.8% of chronic hepatitis patients (P<0.0001). The mean telomere length (MTL) in HCC was significantly shorter compared with chronic hepatitis (P<0.0001). The MTL was not significantly different in HCC patients with and without cirrhosis (P=0.77). In hepatitis B virus, hepatitis C virus and non-B non-C-related HCC groups, no differences were found in telomerase activity and MTL (P=0.77). hTERT, a regulator of telomerase, was, however, positive in 81% of HCCs. The correlation between telomerase activity and hTERT mRNA expression was statistically significant (P<0.0001). The MTL in telomerase-positive HCC cases was significantly shorter than the MTL in telomerase-negative cases (P<0.0001). CONCLUSION: The majority of HCCs exhibited telomerase activity that correlated well with hTERT expression. MTL in HCC was significantly shorter than chronic hepatitis. It was also found that shorter telomeres are present in telomerase-positive HCC cases. However, no correlation was found between telomerase activity and TL with respect to the viral status in HCC. SN - 1478-3231 UR - https://www.unboundmedicine.com/medline/citation/19627485/Telomerase_activity_telomere_length_and_human_telomerase_reverse_transcriptase_expression_in_hepatocellular_carcinoma_is_independent_of_hepatitis_virus_status_ L2 - https://doi.org/10.1111/j.1478-3231.2009.02082.x DB - PRIME DP - Unbound Medicine ER -