Tags

Type your tag names separated by a space and hit enter

Proteomic characterization of copper stress response in Elsholtzia splendens roots and leaves.
Plant Mol Biol. 2009 Oct; 71(3):251-63.PM

Abstract

Elsholtzia splendens is generally considered as a Cu-tolerant and -accumulating plant species, and a candidate for phytoremediation of Cu-contaminated soils. To better understand the Cu tolerance/accumulation mechanisms in E. splendens, proteomic analysis was performed on E. splendens roots and leaves exposed to 100 muM CuSO(4) for 3 and 6 days. After 6 days of treatment, Cu accumulation in roots increased much more than that in leaves. SDS-PAGE analysis showed that the proteins changed more intensively in roots than did in leaves upon Cu stress. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 45 protein spots were significantly changed in roots, but only six protein spots in leaves. The abundance of protein spots mostly showed temporal changes. MALDI-TOF MS and LTQ-ESI-MS/MS were used to identify the differently expressed protein spots. The identified root proteins were involved in various cellular processes such as signal transduction, regulation of transcription and translation, energy metabolism, regulation of redox homeostasis and cell defense. The leaf proteins were mainly degraded fragments of RuBisCo and antioxidative protein. The roles of these proteins in Cu tolerance/accumulation were discussed. The resulting differences in protein expression pattern suggested that redirection of root cellular metabolism and redox homeostasis might be important survival mechanisms of E. splendens upon Cu stress.

Authors+Show Affiliations

Department of Environmental Engineering, Zhejiang University, 310029 Hangzhou, People's Republic of China. lifeng0617@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19629718

Citation

Li, Feng, et al. "Proteomic Characterization of Copper Stress Response in Elsholtzia Splendens Roots and Leaves." Plant Molecular Biology, vol. 71, no. 3, 2009, pp. 251-63.
Li F, Shi J, Shen C, et al. Proteomic characterization of copper stress response in Elsholtzia splendens roots and leaves. Plant Mol Biol. 2009;71(3):251-63.
Li, F., Shi, J., Shen, C., Chen, G., Hu, S., & Chen, Y. (2009). Proteomic characterization of copper stress response in Elsholtzia splendens roots and leaves. Plant Molecular Biology, 71(3), 251-63. https://doi.org/10.1007/s11103-009-9521-y
Li F, et al. Proteomic Characterization of Copper Stress Response in Elsholtzia Splendens Roots and Leaves. Plant Mol Biol. 2009;71(3):251-63. PubMed PMID: 19629718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic characterization of copper stress response in Elsholtzia splendens roots and leaves. AU - Li,Feng, AU - Shi,Jiyan, AU - Shen,Chaofeng, AU - Chen,Guangcun, AU - Hu,Shaoping, AU - Chen,Yingxu, Y1 - 2009/07/24/ PY - 2009/02/13/received PY - 2009/06/27/accepted PY - 2009/7/25/entrez PY - 2009/7/25/pubmed PY - 2009/9/26/medline SP - 251 EP - 63 JF - Plant molecular biology JO - Plant Mol Biol VL - 71 IS - 3 N2 - Elsholtzia splendens is generally considered as a Cu-tolerant and -accumulating plant species, and a candidate for phytoremediation of Cu-contaminated soils. To better understand the Cu tolerance/accumulation mechanisms in E. splendens, proteomic analysis was performed on E. splendens roots and leaves exposed to 100 muM CuSO(4) for 3 and 6 days. After 6 days of treatment, Cu accumulation in roots increased much more than that in leaves. SDS-PAGE analysis showed that the proteins changed more intensively in roots than did in leaves upon Cu stress. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 45 protein spots were significantly changed in roots, but only six protein spots in leaves. The abundance of protein spots mostly showed temporal changes. MALDI-TOF MS and LTQ-ESI-MS/MS were used to identify the differently expressed protein spots. The identified root proteins were involved in various cellular processes such as signal transduction, regulation of transcription and translation, energy metabolism, regulation of redox homeostasis and cell defense. The leaf proteins were mainly degraded fragments of RuBisCo and antioxidative protein. The roles of these proteins in Cu tolerance/accumulation were discussed. The resulting differences in protein expression pattern suggested that redirection of root cellular metabolism and redox homeostasis might be important survival mechanisms of E. splendens upon Cu stress. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/19629718/Proteomic_characterization_of_copper_stress_response_in_Elsholtzia_splendens_roots_and_leaves_ L2 - https://doi.org/10.1007/s11103-009-9521-y DB - PRIME DP - Unbound Medicine ER -