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Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC-DAD-ELSD and HPLC-TOF/MS.
Biomed Chromatogr. 2010 Feb; 24(2):125-31.BC

Abstract

Buyang Huanwu Decoction (BYHYD) is a classical traditional Chinese medicinal prescription that has been widely used for treating cerebrovascular illnesses for hundreds of years. In this study, a comprehensive analytical method has been developed for quantitative analysis of the major constituents in BYHWD. This method was based on high-performance liquid chromatography coupled to a diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) on a common reverse-phase C(18) column. HPLC coupled with on-line time-of-flight mass spectrometry (HPLC-TOF/MS) was additionally adopted to provide further validation for the constituents. It was found that 0.3% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution. This method, which showed excellent precision and accuracy, was successfully applied to quantify the bioactive constituents in six BYHWD products. The validated HPLC-DAD-ELSD method, together with the HPLC-TOF/MS analysis, provided a new basis for assessing the quality of traditional Chinese medicinal prescription consisting of many bioactive components.

Authors+Show Affiliations

Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education; Nanjing, 210009, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19630070

Citation

Liu, E-Hu, et al. "Simultaneous Determination of Twelve Bioactive Constituents in Buyang Huanwu Decoction By HPLC-DAD-ELSD and HPLC-TOF/MS." Biomedical Chromatography : BMC, vol. 24, no. 2, 2010, pp. 125-31.
Liu EH, Qi LW, Cheng XL, et al. Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC-DAD-ELSD and HPLC-TOF/MS. Biomed Chromatogr. 2010;24(2):125-31.
Liu, E. H., Qi, L. W., Cheng, X. L., Peng, Y. B., & Li, P. (2010). Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC-DAD-ELSD and HPLC-TOF/MS. Biomedical Chromatography : BMC, 24(2), 125-31. https://doi.org/10.1002/bmc.1269
Liu EH, et al. Simultaneous Determination of Twelve Bioactive Constituents in Buyang Huanwu Decoction By HPLC-DAD-ELSD and HPLC-TOF/MS. Biomed Chromatogr. 2010;24(2):125-31. PubMed PMID: 19630070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC-DAD-ELSD and HPLC-TOF/MS. AU - Liu,E-Hu, AU - Qi,Lian-Wen, AU - Cheng,Xiao-Lan, AU - Peng,Yong-bo, AU - Li,Ping, PY - 2009/7/25/entrez PY - 2009/7/25/pubmed PY - 2010/3/2/medline SP - 125 EP - 31 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 24 IS - 2 N2 - Buyang Huanwu Decoction (BYHYD) is a classical traditional Chinese medicinal prescription that has been widely used for treating cerebrovascular illnesses for hundreds of years. In this study, a comprehensive analytical method has been developed for quantitative analysis of the major constituents in BYHWD. This method was based on high-performance liquid chromatography coupled to a diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) on a common reverse-phase C(18) column. HPLC coupled with on-line time-of-flight mass spectrometry (HPLC-TOF/MS) was additionally adopted to provide further validation for the constituents. It was found that 0.3% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution. This method, which showed excellent precision and accuracy, was successfully applied to quantify the bioactive constituents in six BYHWD products. The validated HPLC-DAD-ELSD method, together with the HPLC-TOF/MS analysis, provided a new basis for assessing the quality of traditional Chinese medicinal prescription consisting of many bioactive components. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/19630070/Simultaneous_determination_of_twelve_bioactive_constituents_in_Buyang_Huanwu_decoction_by_HPLC_DAD_ELSD_and_HPLC_TOF/MS_ L2 - https://doi.org/10.1002/bmc.1269 DB - PRIME DP - Unbound Medicine ER -