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Screening Pneumocystis carinii pneumonia in non-HIV-infected immunocompromised patients using polymerase chain reaction.
Diagn Microbiol Infect Dis. 2009 Aug; 64(4):396-401.DM

Abstract

We evaluated the clinical value of polymerase chain reaction (PCR) for screening Pneumocystis carinii pneumonia (PCP) in non-HIV-infected immunocompromised patients. We retrospectively analyzed PCR and Grocott's methenamine silver (GMS) staining on 1044 clinical specimens obtained from 756 patients. Positive rates of PCR and GMS staining in sputum specimens were 21.1% and 9.5%, respectively (P < 0.01), and in bronchoalveolar lavage (BAL), specimens were 31.9% and 25.5%, respectively. Among 5 patient groups, the highest GMS staining and PCR positive rates were observed in immunosuppressed patients. In 28 GMS staining-positive patients, positive rates of PCR and GMS staining after a short anti-PCP treatment were 39.3% and 17.9%, respectively. In 5 patients with positive PCR but negative GMS staining results in initial sputum examination, GMS staining result turned positive in subsequent BAL specimens. In conclusion, PCR is sensitive for detection of Pneumocystis in sputum specimens and is useful for screening PCP in non-HIV-infected high-risk patients.

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Authors+Show Affiliations

Jiancheng W
Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
Minjun H
No affiliation info available
Yi-jun A
No affiliation info available
Lan S
No affiliation info available
Zengzhu G
No affiliation info available
Jianrong S
No affiliation info available
Xixiong K
No affiliation info available

MeSH

Bronchoalveolar Lavage FluidHumansImmunocompromised HostMass ScreeningPneumocystis cariniiPneumonia, PneumocystisPolymerase Chain ReactionRetrospective StudiesSensitivity and SpecificitySputumStaining and Labeling

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

19631093

Citation

Jiancheng, Wang, et al. "Screening Pneumocystis Carinii Pneumonia in non-HIV-infected Immunocompromised Patients Using Polymerase Chain Reaction." Diagnostic Microbiology and Infectious Disease, vol. 64, no. 4, 2009, pp. 396-401.
Jiancheng W, Minjun H, Yi-jun A, et al. Screening Pneumocystis carinii pneumonia in non-HIV-infected immunocompromised patients using polymerase chain reaction. Diagn Microbiol Infect Dis. 2009;64(4):396-401.
Jiancheng, W., Minjun, H., Yi-jun, A., Lan, S., Zengzhu, G., Jianrong, S., & Xixiong, K. (2009). Screening Pneumocystis carinii pneumonia in non-HIV-infected immunocompromised patients using polymerase chain reaction. Diagnostic Microbiology and Infectious Disease, 64(4), 396-401. https://doi.org/10.1016/j.diagmicrobio.2009.04.009
Jiancheng W, et al. Screening Pneumocystis Carinii Pneumonia in non-HIV-infected Immunocompromised Patients Using Polymerase Chain Reaction. Diagn Microbiol Infect Dis. 2009;64(4):396-401. PubMed PMID: 19631093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening Pneumocystis carinii pneumonia in non-HIV-infected immunocompromised patients using polymerase chain reaction. AU - Jiancheng,Wang, AU - Minjun,Huang, AU - Yi-jun,An, AU - Lan,Sun, AU - Zengzhu,Guo, AU - Jianrong,Su, AU - Xixiong,Kang, PY - 2009/01/02/received PY - 2009/04/09/revised PY - 2009/04/14/accepted PY - 2009/7/28/entrez PY - 2009/7/28/pubmed PY - 2009/9/22/medline SP - 396 EP - 401 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 64 IS - 4 N2 - We evaluated the clinical value of polymerase chain reaction (PCR) for screening Pneumocystis carinii pneumonia (PCP) in non-HIV-infected immunocompromised patients. We retrospectively analyzed PCR and Grocott's methenamine silver (GMS) staining on 1044 clinical specimens obtained from 756 patients. Positive rates of PCR and GMS staining in sputum specimens were 21.1% and 9.5%, respectively (P < 0.01), and in bronchoalveolar lavage (BAL), specimens were 31.9% and 25.5%, respectively. Among 5 patient groups, the highest GMS staining and PCR positive rates were observed in immunosuppressed patients. In 28 GMS staining-positive patients, positive rates of PCR and GMS staining after a short anti-PCP treatment were 39.3% and 17.9%, respectively. In 5 patients with positive PCR but negative GMS staining results in initial sputum examination, GMS staining result turned positive in subsequent BAL specimens. In conclusion, PCR is sensitive for detection of Pneumocystis in sputum specimens and is useful for screening PCP in non-HIV-infected high-risk patients. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/19631093/Screening_Pneumocystis_carinii_pneumonia_in_non_HIV_infected_immunocompromised_patients_using_polymerase_chain_reaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(09)00149-7 DB - PRIME DP - Unbound Medicine ER -