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Polyclonal presence of non-multiresistant methicillin-resistant Staphylococcus aureus isolates carrying SCCmec IV in health care-associated infections in a hospital in Rio de Janeiro, Brazil.
Diagn Microbiol Infect Dis. 2009 Aug; 64(4):434-41.DM

Abstract

Change in epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) was observed because of the emergence of infections by non-multiresistant MRSA (nMRSA) in our hospital in Rio de Janeiro, Brazil. Clinical characterization and molecular analysis of 20 nMRSA isolates recovered from 17 patients, between February 2005 and March 2006, were performed. The analysis included SCCmec (staphylococcal cassette chromosome mec), pulsed field gel electrophoresis (PFGE), multilocus restriction fragment, and multilocus sequence typing. MICs for oxacillin and vancomycin and presence of Panton-Valentine leukocidin (PVL) genes were also investigated. All but 1 of the 20 isolates presented SCCmec type IV. PFGE clustered all isolates into 9 genotypes. MIC < or = 16 microg/mL to oxacillin was found for 65% of the isolates, whereas 80% exhibited MIC of 2 microg/mL for vancomycin. PVL-encoding genes were observed in 3 isolates. Polyclonal presence of nMRSA SCCmec IV was observed in our institution, including community and health care-associated isolates, which belonged to the sequence types (STs) 1 (clonal complex [CC1]), ST5 (CC5), ST8 and ST72 (CC8), ST97 (CC97), and 2 ST singletons (SLV5 and SLV30).

Authors+Show Affiliations

Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19631097

Citation

Schuenck, Ricardo Pinto, et al. "Polyclonal Presence of Non-multiresistant Methicillin-resistant Staphylococcus Aureus Isolates Carrying SCCmec IV in Health Care-associated Infections in a Hospital in Rio De Janeiro, Brazil." Diagnostic Microbiology and Infectious Disease, vol. 64, no. 4, 2009, pp. 434-41.
Schuenck RP, Nouér SA, Winter Cde O, et al. Polyclonal presence of non-multiresistant methicillin-resistant Staphylococcus aureus isolates carrying SCCmec IV in health care-associated infections in a hospital in Rio de Janeiro, Brazil. Diagn Microbiol Infect Dis. 2009;64(4):434-41.
Schuenck, R. P., Nouér, S. A., Winter, C. d. e. . O., Cavalcante, F. S., Scotti, T. D., Ferreira, A. L., Giambiagi-de Marval, M., & dos Santos, K. R. (2009). Polyclonal presence of non-multiresistant methicillin-resistant Staphylococcus aureus isolates carrying SCCmec IV in health care-associated infections in a hospital in Rio de Janeiro, Brazil. Diagnostic Microbiology and Infectious Disease, 64(4), 434-41. https://doi.org/10.1016/j.diagmicrobio.2009.04.007
Schuenck RP, et al. Polyclonal Presence of Non-multiresistant Methicillin-resistant Staphylococcus Aureus Isolates Carrying SCCmec IV in Health Care-associated Infections in a Hospital in Rio De Janeiro, Brazil. Diagn Microbiol Infect Dis. 2009;64(4):434-41. PubMed PMID: 19631097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyclonal presence of non-multiresistant methicillin-resistant Staphylococcus aureus isolates carrying SCCmec IV in health care-associated infections in a hospital in Rio de Janeiro, Brazil. AU - Schuenck,Ricardo Pinto, AU - Nouér,Simone Aranha, AU - Winter,Carolina de Oliveira, AU - Cavalcante,Fernanda Sampaio, AU - Scotti,Tatiana Dantas, AU - Ferreira,Adriana Lúcia Pires, AU - Giambiagi-de Marval,Márcia, AU - dos Santos,Kátia Regina Netto, PY - 2009/02/09/received PY - 2009/03/30/revised PY - 2009/04/11/accepted PY - 2009/7/28/entrez PY - 2009/7/28/pubmed PY - 2009/9/22/medline SP - 434 EP - 41 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 64 IS - 4 N2 - Change in epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) was observed because of the emergence of infections by non-multiresistant MRSA (nMRSA) in our hospital in Rio de Janeiro, Brazil. Clinical characterization and molecular analysis of 20 nMRSA isolates recovered from 17 patients, between February 2005 and March 2006, were performed. The analysis included SCCmec (staphylococcal cassette chromosome mec), pulsed field gel electrophoresis (PFGE), multilocus restriction fragment, and multilocus sequence typing. MICs for oxacillin and vancomycin and presence of Panton-Valentine leukocidin (PVL) genes were also investigated. All but 1 of the 20 isolates presented SCCmec type IV. PFGE clustered all isolates into 9 genotypes. MIC < or = 16 microg/mL to oxacillin was found for 65% of the isolates, whereas 80% exhibited MIC of 2 microg/mL for vancomycin. PVL-encoding genes were observed in 3 isolates. Polyclonal presence of nMRSA SCCmec IV was observed in our institution, including community and health care-associated isolates, which belonged to the sequence types (STs) 1 (clonal complex [CC1]), ST5 (CC5), ST8 and ST72 (CC8), ST97 (CC97), and 2 ST singletons (SLV5 and SLV30). SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/19631097/Polyclonal_presence_of_non_multiresistant_methicillin_resistant_Staphylococcus_aureus_isolates_carrying_SCCmec_IV_in_health_care_associated_infections_in_a_hospital_in_Rio_de_Janeiro_Brazil_ DB - PRIME DP - Unbound Medicine ER -