Tags

Type your tag names separated by a space and hit enter

Identification and quantification of flavonoids and phenolic acids in burr parsley (Caucalis platycarpos L.), using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.
Molecules. 2009 Jul 09; 14(7):2466-90.M

Abstract

A sensitive method coupling high-performance liquid chromatography (HPLC) with diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was optimized for the separation and identification of phenolic acids, flavonoid glycosides and flavonoid aglycones in the extract of burr parsley (Caucalis platycarpos L.). Fragmentation behavior of flavonoid glycosides and phenolic acids were investigated using ion trap mass spectrometry in negative electrospray ionization. The MS, MS(n) and UV data together with HPLC retention time (T(R)) of phenolic acids and flavonoids allowed structural characterization of these compounds. Caffeoylquinic acid (CQA) isomers, p-coumaroyl-quinic acids (p-CoQA), feruloylquinic acids (FQA), dicaffeoylquinic acids (diCQA), luteolin-7-O-rutinoside, apigenin-7-O-rutinoside as well as isolated chrysoeriol-7-O-rutinoside have been identified as constituents of C. platycarpos for the first time. An accurate, precise and sensitive LC-DAD method for quantification of four phenolic acids (3-O-caffeoylquinic, caffeic, p-coumaric, o-coumaric acid), four flavonoid glycosides (luteolin-7-O-glucoside, apigenin-7-O-glucoside, quercetin-3-O-galactoside, quercetin-3-O-rhamnoside), and three flavonoid aglycones (luteolin, apigenin, chrysoeriol) in C. platycarpos extract was validated in terms of linearity, limit of detection, limit of quantification, precision and accuracy. 3-O-caffeoylquinic acid was the predominant phenolic acid and luteolin-7-O-glucoside was the predominant flavonoid glycoside.

Authors+Show Affiliations

Agency for Medicinal Products and Medical Devices, Ksaverska cesta 4, 10000 Zagreb, Croatia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19633617

Citation

Plazonić, Ana, et al. "Identification and Quantification of Flavonoids and Phenolic Acids in Burr Parsley (Caucalis Platycarpos L.), Using High-performance Liquid Chromatography With Diode Array Detection and Electrospray Ionization Mass Spectrometry." Molecules (Basel, Switzerland), vol. 14, no. 7, 2009, pp. 2466-90.
Plazonić A, Bucar F, Males Z, et al. Identification and quantification of flavonoids and phenolic acids in burr parsley (Caucalis platycarpos L.), using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry. Molecules. 2009;14(7):2466-90.
Plazonić, A., Bucar, F., Males, Z., Mornar, A., Nigović, B., & Kujundzić, N. (2009). Identification and quantification of flavonoids and phenolic acids in burr parsley (Caucalis platycarpos L.), using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry. Molecules (Basel, Switzerland), 14(7), 2466-90. https://doi.org/10.3390/molecules14072466
Plazonić A, et al. Identification and Quantification of Flavonoids and Phenolic Acids in Burr Parsley (Caucalis Platycarpos L.), Using High-performance Liquid Chromatography With Diode Array Detection and Electrospray Ionization Mass Spectrometry. Molecules. 2009 Jul 9;14(7):2466-90. PubMed PMID: 19633617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and quantification of flavonoids and phenolic acids in burr parsley (Caucalis platycarpos L.), using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry. AU - Plazonić,Ana, AU - Bucar,Franz, AU - Males,Zeljan, AU - Mornar,Ana, AU - Nigović,Biljana, AU - Kujundzić,Nikola, Y1 - 2009/07/09/ PY - 2009/06/08/received PY - 2009/06/25/revised PY - 2009/07/06/accepted PY - 2009/7/28/entrez PY - 2009/7/28/pubmed PY - 2009/10/9/medline SP - 2466 EP - 90 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 14 IS - 7 N2 - A sensitive method coupling high-performance liquid chromatography (HPLC) with diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was optimized for the separation and identification of phenolic acids, flavonoid glycosides and flavonoid aglycones in the extract of burr parsley (Caucalis platycarpos L.). Fragmentation behavior of flavonoid glycosides and phenolic acids were investigated using ion trap mass spectrometry in negative electrospray ionization. The MS, MS(n) and UV data together with HPLC retention time (T(R)) of phenolic acids and flavonoids allowed structural characterization of these compounds. Caffeoylquinic acid (CQA) isomers, p-coumaroyl-quinic acids (p-CoQA), feruloylquinic acids (FQA), dicaffeoylquinic acids (diCQA), luteolin-7-O-rutinoside, apigenin-7-O-rutinoside as well as isolated chrysoeriol-7-O-rutinoside have been identified as constituents of C. platycarpos for the first time. An accurate, precise and sensitive LC-DAD method for quantification of four phenolic acids (3-O-caffeoylquinic, caffeic, p-coumaric, o-coumaric acid), four flavonoid glycosides (luteolin-7-O-glucoside, apigenin-7-O-glucoside, quercetin-3-O-galactoside, quercetin-3-O-rhamnoside), and three flavonoid aglycones (luteolin, apigenin, chrysoeriol) in C. platycarpos extract was validated in terms of linearity, limit of detection, limit of quantification, precision and accuracy. 3-O-caffeoylquinic acid was the predominant phenolic acid and luteolin-7-O-glucoside was the predominant flavonoid glycoside. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/19633617/Identification_and_quantification_of_flavonoids_and_phenolic_acids_in_burr_parsley__Caucalis_platycarpos_L___using_high_performance_liquid_chromatography_with_diode_array_detection_and_electrospray_ionization_mass_spectrometry_ L2 - https://www.mdpi.com/resolver?pii=14072466 DB - PRIME DP - Unbound Medicine ER -