Prime

Type your tag names separated by a space and hit enter

Simultaneous determination of oxyresveratrol and resveratrol in rat bile and urine by HPLC after oral administration of Smilax china extract.

Abstract

Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 x 4.6 mm, 5 microm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively.

Authors+Show Affiliations

,

Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, PR China.

, , , ,

Source

Natural product communications 4:6 2009 Jun pg 825-30

MeSH

Administration, Oral
Animals
Bile
Chromatography, High Pressure Liquid
Female
Plant Extracts
Rats
Rats, Sprague-Dawley
Resveratrol
Sensitivity and Specificity
Smilax
Stilbenes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19634330

Citation

TY - JOUR T1 - Simultaneous determination of oxyresveratrol and resveratrol in rat bile and urine by HPLC after oral administration of Smilax china extract. AU - Huang,Hui-lian, AU - Zhang,Jin-qiang, AU - Chena,Guang-tong, AU - Lu,Zhi-qiang, AU - Sha,Na, AU - Guo,De-an, PY - 2009/7/29/entrez PY - 2009/7/29/pubmed PY - 2009/9/9/medline SP - 825 EP - 30 JF - Natural product communications JO - Nat Prod Commun VL - 4 IS - 6 N2 - Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 x 4.6 mm, 5 microm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/19634330/Simultaneous_determination_of_oxyresveratrol_and_resveratrol_in_rat_bile_and_urine_by_HPLC_after_oral_administration_of_Smilax_china_extract_ ER -