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Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by microcystin-LR.
J Environ Sci (China). 2009; 21(3):395-401.JE

Abstract

Among more than 75 variants of microcystin (MC), microcystin-LR (MC-LR) is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 Ig/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation time, decrease brood size, suppress locomotion behavior, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp-16-2-gfp were very sensitive to 1.0 microg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp-16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily affected by MC-LR. Therefore, the generation time, brood size, and hsp-16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure.

Authors+Show Affiliations

Li Y
School of Public Health, Southeast University, Nanjing 210009, China. lyh1216@126.com
Wang Y
No affiliation info available
Yin L
No affiliation info available
Pu Y
No affiliation info available
Wang D
No affiliation info available

MeSH

AnimalsAnimals, Genetically ModifiedBehavior, AnimalBody SizeCaenorhabditis elegansCaenorhabditis elegans ProteinsEnzyme InhibitorsHeat-Shock ProteinsHumansMarine ToxinsMicrocystinsMotor ActivityRecombinant Fusion Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19634454

Citation

Li, Yunhui, et al. "Using the Nematode Caenorhabditis Elegans as a Model Animal for Assessing the Toxicity Induced By Microcystin-LR." Journal of Environmental Sciences (China), vol. 21, no. 3, 2009, pp. 395-401.
Li Y, Wang Y, Yin L, et al. Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by microcystin-LR. J Environ Sci (China). 2009;21(3):395-401.
Li, Y., Wang, Y., Yin, L., Pu, Y., & Wang, D. (2009). Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by microcystin-LR. Journal of Environmental Sciences (China), 21(3), 395-401.
Li Y, et al. Using the Nematode Caenorhabditis Elegans as a Model Animal for Assessing the Toxicity Induced By Microcystin-LR. J Environ Sci (China). 2009;21(3):395-401. PubMed PMID: 19634454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by microcystin-LR. AU - Li,Yunhui, AU - Wang,Yang, AU - Yin,Lihong, AU - Pu,Yuepu, AU - Wang,Dayong, PY - 2009/7/29/entrez PY - 2009/7/29/pubmed PY - 2009/8/11/medline SP - 395 EP - 401 JF - Journal of environmental sciences (China) JO - J Environ Sci (China) VL - 21 IS - 3 N2 - Among more than 75 variants of microcystin (MC), microcystin-LR (MC-LR) is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 Ig/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation time, decrease brood size, suppress locomotion behavior, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp-16-2-gfp were very sensitive to 1.0 microg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp-16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily affected by MC-LR. Therefore, the generation time, brood size, and hsp-16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure. SN - 1001-0742 UR - https://www.unboundmedicine.com/medline/citation/19634454/Using_the_nematode_Caenorhabditis_elegans_as_a_model_animal_for_assessing_the_toxicity_induced_by_microcystin_LR_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:19634454 DB - PRIME DP - Unbound Medicine ER -