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Stable oil bodies sheltered by a unique caleosin in cycad megagametophytes.
Plant Physiol Biochem. 2009 Nov-Dec; 47(11-12):1009-16.PP

Abstract

Stable oil bodies of smaller sizes and higher thermostability were isolated from mature cycad (Cycas revoluta) megagametophytes compared with those isolated from sesame seeds. Immunological cross-recognition revealed that cycad oil bodies contained a major protein of 27 kDa, tentatively identified as caleosin, while oleosin, the well-known structural protein, was apparently absent. Mass spectrometric analysis showed that the putative cycad caleosin possessed a tryptic fragment of 15 residues matching to that of a theoretical moss caleosin. A complete cDNA fragment encoding this putative caleosin was obtained by PCR cloning using a primer designed according to the tryptic peptide and another one designed according to a highly conservative region among diverse caleosins. The identification of this clone was subsequently confirmed by immunodetection and MALDI-MS analyses of its recombinant fusion protein over-expressed in Escherichia coli and the native form from cycad oil bodies. Stable artificial oil bodies were successfully constituted with triacylglycerol, phospholipid and the recombinant fusion protein containing the cycad caleosin. These results suggest that stable oil bodies in cycad megagametophytes are mainly sheltered by a unique structural protein caleosin.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19635673

Citation

Jiang, Pei-Luen, et al. "Stable Oil Bodies Sheltered By a Unique Caleosin in Cycad Megagametophytes." Plant Physiology and Biochemistry : PPB, vol. 47, no. 11-12, 2009, pp. 1009-16.
Jiang PL, Chen JC, Chiu ST, et al. Stable oil bodies sheltered by a unique caleosin in cycad megagametophytes. Plant Physiol Biochem. 2009;47(11-12):1009-16.
Jiang, P. L., Chen, J. C., Chiu, S. T., & Tzen, J. T. (2009). Stable oil bodies sheltered by a unique caleosin in cycad megagametophytes. Plant Physiology and Biochemistry : PPB, 47(11-12), 1009-16. https://doi.org/10.1016/j.plaphy.2009.07.004
Jiang PL, et al. Stable Oil Bodies Sheltered By a Unique Caleosin in Cycad Megagametophytes. Plant Physiol Biochem. 2009 Nov-Dec;47(11-12):1009-16. PubMed PMID: 19635673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stable oil bodies sheltered by a unique caleosin in cycad megagametophytes. AU - Jiang,Pei-Luen, AU - Chen,Jeff C F, AU - Chiu,Shau-Ting, AU - Tzen,Jason T C, Y1 - 2009/07/15/ PY - 2009/05/27/received PY - 2009/07/02/revised PY - 2009/07/05/accepted PY - 2009/7/29/entrez PY - 2009/7/29/pubmed PY - 2010/3/27/medline SP - 1009 EP - 16 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 47 IS - 11-12 N2 - Stable oil bodies of smaller sizes and higher thermostability were isolated from mature cycad (Cycas revoluta) megagametophytes compared with those isolated from sesame seeds. Immunological cross-recognition revealed that cycad oil bodies contained a major protein of 27 kDa, tentatively identified as caleosin, while oleosin, the well-known structural protein, was apparently absent. Mass spectrometric analysis showed that the putative cycad caleosin possessed a tryptic fragment of 15 residues matching to that of a theoretical moss caleosin. A complete cDNA fragment encoding this putative caleosin was obtained by PCR cloning using a primer designed according to the tryptic peptide and another one designed according to a highly conservative region among diverse caleosins. The identification of this clone was subsequently confirmed by immunodetection and MALDI-MS analyses of its recombinant fusion protein over-expressed in Escherichia coli and the native form from cycad oil bodies. Stable artificial oil bodies were successfully constituted with triacylglycerol, phospholipid and the recombinant fusion protein containing the cycad caleosin. These results suggest that stable oil bodies in cycad megagametophytes are mainly sheltered by a unique structural protein caleosin. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/19635673/Stable_oil_bodies_sheltered_by_a_unique_caleosin_in_cycad_megagametophytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(09)00167-3 DB - PRIME DP - Unbound Medicine ER -