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Assessment of the genetic stability of micropropagated plants of Cannabis sativa by ISSR markers.

Abstract

Inter-simple sequence repeat (ISSR) markers were used to evaluate the genetic stability of the micropropagated plants of Cannabis sativa over 30 passages in culture and hardening in soil for 8 months. A total of 15 ISSR primers resulted in 115 distinct and reproducible bands. All the ISSR profiles from micropropagated plants were monomorphic and comparable to mother plants, confirming the genetic stability among clones and mother plants. Chemical analysis of cannabinoids, using gas chromatography/flame ionization detection (GC/FID), was done to further confirm whether the qualitative and quantitative differences in the major secondary metabolites exist between the mother plant and micropropagated plants. Six major cannabinoids - Delta(9)-THC, THCV, CBD, CBC, CBG, and CBN - were identified and compared with the mother plant. Our results clearly showed a similar cannabinoid profile and insignificant differences in THC content between the two types of plants. These results suggest that the micropropagation protocol developed by us for rapid IN VITRO multiplication is appropriate and applicable for clonal mass propagation of C. SATIVA.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. hlata@olemiss.edu

    , , ,

    Source

    Planta medica 76:1 2010 Jan pg 97-100

    MeSH

    Base Sequence
    Cannabinoids
    Cannabis
    DNA, Plant
    Dronabinol
    Genetic Markers
    Plant Extracts
    Reproduction, Asexual

    Pub Type(s)

    Letter
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    19637112

    Citation

    TY - JOUR T1 - Assessment of the genetic stability of micropropagated plants of Cannabis sativa by ISSR markers. AU - Lata,Hemant, AU - Chandra,Suman, AU - Techen,Natascha, AU - Khan,Ikhlas A, AU - ElSohly,Mahmoud A, Y1 - 2009/07/27/ PY - 2009/7/29/entrez PY - 2009/7/29/pubmed PY - 2010/5/22/medline SP - 97 EP - 100 JF - Planta medica JO - Planta Med. VL - 76 IS - 1 N2 - Inter-simple sequence repeat (ISSR) markers were used to evaluate the genetic stability of the micropropagated plants of Cannabis sativa over 30 passages in culture and hardening in soil for 8 months. A total of 15 ISSR primers resulted in 115 distinct and reproducible bands. All the ISSR profiles from micropropagated plants were monomorphic and comparable to mother plants, confirming the genetic stability among clones and mother plants. Chemical analysis of cannabinoids, using gas chromatography/flame ionization detection (GC/FID), was done to further confirm whether the qualitative and quantitative differences in the major secondary metabolites exist between the mother plant and micropropagated plants. Six major cannabinoids - Delta(9)-THC, THCV, CBD, CBC, CBG, and CBN - were identified and compared with the mother plant. Our results clearly showed a similar cannabinoid profile and insignificant differences in THC content between the two types of plants. These results suggest that the micropropagation protocol developed by us for rapid IN VITRO multiplication is appropriate and applicable for clonal mass propagation of C. SATIVA. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/19637112/abstract/Assessment_of_the_Genetic_Stability_of_Micropropagated_Plants_of_Cannabis_sativa_by_ISSR_Markers_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1185945 ER -