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Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection.
J Invertebr Pathol. 2009 Oct; 102(2):129-32.JI

Abstract

Paenibacillus larvae is the causative agent of American Foulbrood (AFB), the most severe bacterial disease that affects honeybee larvae. AFB causes a significant decrease in the honeybee population affecting the beekeeping industry and agricultural production. After infection of larvae, P. larvae secretes proteases that could be involved in the pathogenicity. In the present article, we present the secretion of different proteases by P. larvae. Inhibition assays confirmed the presence of metalloproteases. Two different proteases patterns (PP1 and PP2) were identified in a collection of P. larvae isolates from different geographic origin. Forty nine percent of P. larvae isolates showed pattern PP1 while 51% exhibited pattern PP2. Most isolates belonging to genotype ERIC I - BOX A presented PP2, most isolates belonging to ERIC I - BOX C presented PP1 although relations were not significant. Isolates belonging to genotypes ERIC II and ERIC III presented PP2. No correlation was observed between the secreted proteases patterns and geographic distribution, since both patterns are widely distributed in Uruguay. According to exposure bioassays, isolates showing PP2 are more virulent than those showing PP1, suggesting that difference in pathogenicity could be related to the secretion of proteases.

Authors+Show Affiliations

Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, CP 11600 Montevideo, Uruguay. karina@iibce.edu.uyNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19638278

Citation

Antúnez, Karina, et al. "Characterization of Secreted Proteases of Paenibacillus Larvae, Potential Virulence Factors Involved in Honeybee Larval Infection." Journal of Invertebrate Pathology, vol. 102, no. 2, 2009, pp. 129-32.
Antúnez K, Anido M, Schlapp G, et al. Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection. J Invertebr Pathol. 2009;102(2):129-32.
Antúnez, K., Anido, M., Schlapp, G., Evans, J. D., & Zunino, P. (2009). Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection. Journal of Invertebrate Pathology, 102(2), 129-32. https://doi.org/10.1016/j.jip.2009.07.010
Antúnez K, et al. Characterization of Secreted Proteases of Paenibacillus Larvae, Potential Virulence Factors Involved in Honeybee Larval Infection. J Invertebr Pathol. 2009;102(2):129-32. PubMed PMID: 19638278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection. AU - Antúnez,Karina, AU - Anido,Matilde, AU - Schlapp,Geraldine, AU - Evans,Jay D, AU - Zunino,Pablo, Y1 - 2009/07/26/ PY - 2009/03/27/received PY - 2009/06/26/revised PY - 2009/07/12/accepted PY - 2009/7/30/entrez PY - 2009/7/30/pubmed PY - 2009/12/16/medline SP - 129 EP - 32 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 102 IS - 2 N2 - Paenibacillus larvae is the causative agent of American Foulbrood (AFB), the most severe bacterial disease that affects honeybee larvae. AFB causes a significant decrease in the honeybee population affecting the beekeeping industry and agricultural production. After infection of larvae, P. larvae secretes proteases that could be involved in the pathogenicity. In the present article, we present the secretion of different proteases by P. larvae. Inhibition assays confirmed the presence of metalloproteases. Two different proteases patterns (PP1 and PP2) were identified in a collection of P. larvae isolates from different geographic origin. Forty nine percent of P. larvae isolates showed pattern PP1 while 51% exhibited pattern PP2. Most isolates belonging to genotype ERIC I - BOX A presented PP2, most isolates belonging to ERIC I - BOX C presented PP1 although relations were not significant. Isolates belonging to genotypes ERIC II and ERIC III presented PP2. No correlation was observed between the secreted proteases patterns and geographic distribution, since both patterns are widely distributed in Uruguay. According to exposure bioassays, isolates showing PP2 are more virulent than those showing PP1, suggesting that difference in pathogenicity could be related to the secretion of proteases. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/19638278/Characterization_of_secreted_proteases_of_Paenibacillus_larvae_potential_virulence_factors_involved_in_honeybee_larval_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(09)00149-9 DB - PRIME DP - Unbound Medicine ER -