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[Determination of ephedrine and pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang by capillary zone electrophoresis].
Zhongguo Zhong Yao Za Zhi. 2009 Apr; 34(8):980-3.ZZ

Abstract

OBJECTIVE

To establish a method for the determination of ephedrine and pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang by capillary zone electrophoresis.

METHOD

The conditions of the experiment were optimized with a fused-silica capillary of 60 cm x 50 microm (50 cm effective length) in a running buffer of 50 mmol x L(-1) borax-20 mmol x L(-1) threonine (pH 9.27) and an applied voltage of 15 kV (room temperature). Samples were introduced by hydrodynamic injections (10 cm x 20 s)and determined with on-column UV monitoring at 210 nm. Phenobarbital was chosen as the internal standard.

RESULT

Ephedrine and pseudoephedrine are separated successfully within 8 min. The linear responses covered the ranges from 21.3 to 213 mg x L(-1) (r = 0.9996) for ephedrine and from 8.4 to 84 mg x L(-1) (r = 0.9995) for pseudoephedrine. The detection limits (S/N = 3) of ephedrine and pseudoephedrine were shown to be 1.45 and 1.48 microg x mL(-1), respectively, The quantitation limits (S/N = 10) of ephedrine and pseudoephedrine were shown to be 4.81 and 4.93 mg x L(-1), respectively. The average recoveries for ephedrine and pseudoephedrine were 97.5% and 98.6% with RSD less than 5.0%.

CONCLUSION

The method is simple, rapid, cost-effective and precise with satisfactory results.

Authors+Show Affiliations

Hebei Key Laboratory of Drug Quality Research, School of Pharmacy, Hebei University, Baoding 071002, China. haoranjing1980@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19639780

Citation

Jing, Haoran, et al. "[Determination of Ephedrine and Pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang By Capillary Zone Electrophoresis]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 34, no. 8, 2009, pp. 980-3.
Jing H, Guo H, Wang Z, et al. [Determination of ephedrine and pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang by capillary zone electrophoresis]. Zhongguo Zhong Yao Za Zhi. 2009;34(8):980-3.
Jing, H., Guo, H., Wang, Z., Wang, M., & Zhang, B. (2009). [Determination of ephedrine and pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang by capillary zone electrophoresis]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 34(8), 980-3.
Jing H, et al. [Determination of Ephedrine and Pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang By Capillary Zone Electrophoresis]. Zhongguo Zhong Yao Za Zhi. 2009;34(8):980-3. PubMed PMID: 19639780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of ephedrine and pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang by capillary zone electrophoresis]. AU - Jing,Haoran, AU - Guo,Huaizhong, AU - Wang,Zijun, AU - Wang,Min, AU - Zhang,Bin, PY - 2009/7/31/entrez PY - 2009/7/31/pubmed PY - 2009/10/10/medline SP - 980 EP - 3 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 34 IS - 8 N2 - OBJECTIVE: To establish a method for the determination of ephedrine and pseudoephedrine in Herba Ephedrae and Maxing Shigan Tang by capillary zone electrophoresis. METHOD: The conditions of the experiment were optimized with a fused-silica capillary of 60 cm x 50 microm (50 cm effective length) in a running buffer of 50 mmol x L(-1) borax-20 mmol x L(-1) threonine (pH 9.27) and an applied voltage of 15 kV (room temperature). Samples were introduced by hydrodynamic injections (10 cm x 20 s)and determined with on-column UV monitoring at 210 nm. Phenobarbital was chosen as the internal standard. RESULT: Ephedrine and pseudoephedrine are separated successfully within 8 min. The linear responses covered the ranges from 21.3 to 213 mg x L(-1) (r = 0.9996) for ephedrine and from 8.4 to 84 mg x L(-1) (r = 0.9995) for pseudoephedrine. The detection limits (S/N = 3) of ephedrine and pseudoephedrine were shown to be 1.45 and 1.48 microg x mL(-1), respectively, The quantitation limits (S/N = 10) of ephedrine and pseudoephedrine were shown to be 4.81 and 4.93 mg x L(-1), respectively. The average recoveries for ephedrine and pseudoephedrine were 97.5% and 98.6% with RSD less than 5.0%. CONCLUSION: The method is simple, rapid, cost-effective and precise with satisfactory results. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/19639780/[Determination_of_ephedrine_and_pseudoephedrine_in_Herba_Ephedrae_and_Maxing_Shigan_Tang_by_capillary_zone_electrophoresis]_ DB - PRIME DP - Unbound Medicine ER -