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Differences in adipocytokines and fatty acid composition between two adipocyte fractions of small and large cells in high-fat diet-induced obese mice.
Ann Nutr Metab. 2009; 54(4):258-67.AN

Abstract

OBJECTIVE

This study was conducted to compare the differences in adipocytokines and fatty acid composition between two fraction sizes of small and large cells from subcutaneous and visceral fat of mice receiving a high-fat diet (HFD).

METHODS

Body weight, blood glucose and cholesterol levels, and adipocyte diameter distribution were examined. Total adipocyte fractions could be separated into the small-cell fraction (SCF) and the large-cell fraction (LCF). Adipocytokine expression, fatty acid composition and stearoyl-CoA desaturase (SCD) expression were compared between SCF and LCF.

RESULTS

In HFD mice, body weight, glucose and cholesterol levels were significantly higher than in NCD mice. The adipocyte diameter of HFD mice continuously increased throughout the feeding period regardless of the fat depot. The expression of adipocytokine genes such as angiotensinogen, plasminogen activator inhibitor-1, adipsin, leptin, TNF-alpha and adiponectin was higher in LCF than SCF. The ratio of stearic acid to the total fatty acid content was lower in SCF than LCF, whereas oleic acid composition was higher in LCF. SCD expression was higher in LCF than SCF.

CONCLUSION

Our results indicate that large adipocytes strongly express adipocytokine genes and show high oleic acid composition via SCD upregulation. Based on these findings, the differences in fatty acid composition between adipocyte sizes may be related to adipocytokine expression.

Authors+Show Affiliations

Division of Cell Regeneration and Transplantation, Advanced Medical Research Center, Nihon University School of Medicine, 30-1 Ohyaguchi-Kamimachi Itabashi-ku, Tokyo 173-8610, Japan. tmatsu@med.nihon-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19641303

Citation

Matsubara, Yoshiyuki, et al. "Differences in Adipocytokines and Fatty Acid Composition Between Two Adipocyte Fractions of Small and Large Cells in High-fat Diet-induced Obese Mice." Annals of Nutrition & Metabolism, vol. 54, no. 4, 2009, pp. 258-67.
Matsubara Y, Kano K, Kondo D, et al. Differences in adipocytokines and fatty acid composition between two adipocyte fractions of small and large cells in high-fat diet-induced obese mice. Ann Nutr Metab. 2009;54(4):258-67.
Matsubara, Y., Kano, K., Kondo, D., Mugishima, H., & Matsumoto, T. (2009). Differences in adipocytokines and fatty acid composition between two adipocyte fractions of small and large cells in high-fat diet-induced obese mice. Annals of Nutrition & Metabolism, 54(4), 258-67. https://doi.org/10.1159/000229506
Matsubara Y, et al. Differences in Adipocytokines and Fatty Acid Composition Between Two Adipocyte Fractions of Small and Large Cells in High-fat Diet-induced Obese Mice. Ann Nutr Metab. 2009;54(4):258-67. PubMed PMID: 19641303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in adipocytokines and fatty acid composition between two adipocyte fractions of small and large cells in high-fat diet-induced obese mice. AU - Matsubara,Yoshiyuki, AU - Kano,Koichiro, AU - Kondo,Daisuke, AU - Mugishima,Hideo, AU - Matsumoto,Taro, Y1 - 2009/07/27/ PY - 2008/06/03/received PY - 2009/05/06/accepted PY - 2009/7/31/entrez PY - 2009/7/31/pubmed PY - 2010/1/13/medline SP - 258 EP - 67 JF - Annals of nutrition & metabolism JO - Ann Nutr Metab VL - 54 IS - 4 N2 - OBJECTIVE: This study was conducted to compare the differences in adipocytokines and fatty acid composition between two fraction sizes of small and large cells from subcutaneous and visceral fat of mice receiving a high-fat diet (HFD). METHODS: Body weight, blood glucose and cholesterol levels, and adipocyte diameter distribution were examined. Total adipocyte fractions could be separated into the small-cell fraction (SCF) and the large-cell fraction (LCF). Adipocytokine expression, fatty acid composition and stearoyl-CoA desaturase (SCD) expression were compared between SCF and LCF. RESULTS: In HFD mice, body weight, glucose and cholesterol levels were significantly higher than in NCD mice. The adipocyte diameter of HFD mice continuously increased throughout the feeding period regardless of the fat depot. The expression of adipocytokine genes such as angiotensinogen, plasminogen activator inhibitor-1, adipsin, leptin, TNF-alpha and adiponectin was higher in LCF than SCF. The ratio of stearic acid to the total fatty acid content was lower in SCF than LCF, whereas oleic acid composition was higher in LCF. SCD expression was higher in LCF than SCF. CONCLUSION: Our results indicate that large adipocytes strongly express adipocytokine genes and show high oleic acid composition via SCD upregulation. Based on these findings, the differences in fatty acid composition between adipocyte sizes may be related to adipocytokine expression. SN - 1421-9697 UR - https://www.unboundmedicine.com/medline/citation/19641303/Differences_in_adipocytokines_and_fatty_acid_composition_between_two_adipocyte_fractions_of_small_and_large_cells_in_high_fat_diet_induced_obese_mice_ L2 - https://www.karger.com?DOI=10.1159/000229506 DB - PRIME DP - Unbound Medicine ER -