Tags

Type your tag names separated by a space and hit enter

Intraarterially delivered human umbilical cord blood-derived mesenchymal stem cells in canine cerebral ischemia.
J Neurosci Res 2009; 87(16):3554-67JN

Abstract

The present study examined the effects of human umbilical cord blood-derived mesenchymal stem cells (HUCB-derived MSCs) delivered through the basilar artery in a canine thromboembolic brain ischemia model. Cerebral ischemia was induced through occlusion of the middle cerebral artery by injecting thrombus emboli into 10 beagles. In the HUCBC group (n = 5), 1 x 10(6) HUCB-derived MSCs were transplanted through the basilar artery 1 day after ischemic induction using an endovascular interventional approach. In the control group (n = 5), phosphate-buffered saline (PBS) was injected in the same manner in as the HUCBC group. Upon neurobehavioral examination, earlier recovery was observed in the HUCBC group. The HUCBC group showed a decrease in the infarction volume at 1 week after cerebral ischemic induction, whereas the control group showed an increase in the infarction volume at 1 week, by magnetic resonance image analysis. Transplanted cells had differentiated into neurons and astrocytes and were observed in and around endothelial cells that were positive for von Willebrand factor (vWF). HUCB-derived MSCs expressed neuroprotective factors, such as brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF), at 4 weeks after the transplantation. The transplanted cells demonstrated their efficacy by reducing the infarction lesion volume and through earlier recovery from the neurological deficit. These results suggest that intraarterial transplantation of HUCB-derived MSCs could be useful in clinical treatment of cerebral ischemia.

Authors+Show Affiliations

Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19642203

Citation

Chung, Dai-Jung, et al. "Intraarterially Delivered Human Umbilical Cord Blood-derived Mesenchymal Stem Cells in Canine Cerebral Ischemia." Journal of Neuroscience Research, vol. 87, no. 16, 2009, pp. 3554-67.
Chung DJ, Choi CB, Lee SH, et al. Intraarterially delivered human umbilical cord blood-derived mesenchymal stem cells in canine cerebral ischemia. J Neurosci Res. 2009;87(16):3554-67.
Chung, D. J., Choi, C. B., Lee, S. H., Kang, E. H., Lee, J. H., Hwang, S. H., ... Kim, H. Y. (2009). Intraarterially delivered human umbilical cord blood-derived mesenchymal stem cells in canine cerebral ischemia. Journal of Neuroscience Research, 87(16), pp. 3554-67. doi:10.1002/jnr.22162.
Chung DJ, et al. Intraarterially Delivered Human Umbilical Cord Blood-derived Mesenchymal Stem Cells in Canine Cerebral Ischemia. J Neurosci Res. 2009;87(16):3554-67. PubMed PMID: 19642203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraarterially delivered human umbilical cord blood-derived mesenchymal stem cells in canine cerebral ischemia. AU - Chung,Dai-Jung, AU - Choi,Chi-Bong, AU - Lee,Sung-Ho, AU - Kang,Eun-Hee, AU - Lee,Jae-Hoon, AU - Hwang,Soo-Han, AU - Han,Hoon, AU - Lee,Jong-Hwan, AU - Choe,Bo-Young, AU - Lee,Soo-Yeol, AU - Kim,Hwi-Yool, PY - 2009/7/31/entrez PY - 2009/7/31/pubmed PY - 2010/1/6/medline SP - 3554 EP - 67 JF - Journal of neuroscience research JO - J. Neurosci. Res. VL - 87 IS - 16 N2 - The present study examined the effects of human umbilical cord blood-derived mesenchymal stem cells (HUCB-derived MSCs) delivered through the basilar artery in a canine thromboembolic brain ischemia model. Cerebral ischemia was induced through occlusion of the middle cerebral artery by injecting thrombus emboli into 10 beagles. In the HUCBC group (n = 5), 1 x 10(6) HUCB-derived MSCs were transplanted through the basilar artery 1 day after ischemic induction using an endovascular interventional approach. In the control group (n = 5), phosphate-buffered saline (PBS) was injected in the same manner in as the HUCBC group. Upon neurobehavioral examination, earlier recovery was observed in the HUCBC group. The HUCBC group showed a decrease in the infarction volume at 1 week after cerebral ischemic induction, whereas the control group showed an increase in the infarction volume at 1 week, by magnetic resonance image analysis. Transplanted cells had differentiated into neurons and astrocytes and were observed in and around endothelial cells that were positive for von Willebrand factor (vWF). HUCB-derived MSCs expressed neuroprotective factors, such as brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF), at 4 weeks after the transplantation. The transplanted cells demonstrated their efficacy by reducing the infarction lesion volume and through earlier recovery from the neurological deficit. These results suggest that intraarterial transplantation of HUCB-derived MSCs could be useful in clinical treatment of cerebral ischemia. SN - 1097-4547 UR - https://www.unboundmedicine.com/medline/citation/19642203/Intraarterially_delivered_human_umbilical_cord_blood_derived_mesenchymal_stem_cells_in_canine_cerebral_ischemia_ L2 - https://doi.org/10.1002/jnr.22162 DB - PRIME DP - Unbound Medicine ER -