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Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering.
J Biomed Mater Res A 2010; 93(2):753-62JB

Abstract

Polymeric nanofiber matrices have already been widely used in tissue engineering. However, the fabrication of nanofibers into complex three-dimensional (3D) structures is restricted due to current manufacturing techniques. To overcome this limitation, we have incorporated nanofibers onto spiral-structured 3D scaffolds made of poly (epsilon-caprolactone) (PCL). The spiral structure with open geometries, large surface areas, and porosity will be helpful for improving nutrient transport and cell penetration into the scaffolds, which are otherwise limited in conventional tissue-engineered scaffolds for large bone defects repair. To investigate the effect of structure and fiber coating on the performance of the scaffolds, three groups of scaffolds including cylindrical PCL scaffolds, spiral PCL scaffolds (without fiber coating), and spiral-structured fibrous PCL scaffolds (with fiber coating) have been prepared. The morphology, porosity, and mechanical properties of the scaffolds have been characterized. Furthermore, human osteoblast cells are seeded on these scaffolds, and the cell attachment, proliferation, differentiation, and mineralized matrix deposition on the scaffolds are evaluated. The results indicated that the spiral scaffolds possess porosities within the range of human trabecular bone and an appropriate pore structure for cell growth, and significantly lower compressive modulus and strength than cylindrical scaffolds. When compared with the cylindrical scaffolds, the spiral-structured scaffolds demonstrated enhanced cell proliferation, differentiation, and mineralization and allowed better cellular growth and penetration. The incorporation of nanofibers onto spiral scaffolds further enhanced cell attachment, proliferation, and differentiation. These studies suggest that spiral-structured nanofibrous scaffolds may serve as promising alternatives for bone tissue engineering applications.

Authors+Show Affiliations

Department of Chemical, Biomedical and Materials Engineering, Stevens Institute of Technology, Hoboken, New Jersey 07030, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

19642211

Citation

Wang, Junping, et al. "Spiral-structured, Nanofibrous, 3D Scaffolds for Bone Tissue Engineering." Journal of Biomedical Materials Research. Part A, vol. 93, no. 2, 2010, pp. 753-62.
Wang J, Valmikinathan CM, Liu W, et al. Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering. J Biomed Mater Res A. 2010;93(2):753-62.
Wang, J., Valmikinathan, C. M., Liu, W., Laurencin, C. T., & Yu, X. (2010). Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering. Journal of Biomedical Materials Research. Part A, 93(2), pp. 753-62. doi:10.1002/jbm.a.32591.
Wang J, et al. Spiral-structured, Nanofibrous, 3D Scaffolds for Bone Tissue Engineering. J Biomed Mater Res A. 2010;93(2):753-62. PubMed PMID: 19642211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering. AU - Wang,Junping, AU - Valmikinathan,Chandra M, AU - Liu,Wei, AU - Laurencin,Cato T, AU - Yu,Xiaojun, PY - 2009/7/31/entrez PY - 2009/7/31/pubmed PY - 2010/6/16/medline SP - 753 EP - 62 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 93 IS - 2 N2 - Polymeric nanofiber matrices have already been widely used in tissue engineering. However, the fabrication of nanofibers into complex three-dimensional (3D) structures is restricted due to current manufacturing techniques. To overcome this limitation, we have incorporated nanofibers onto spiral-structured 3D scaffolds made of poly (epsilon-caprolactone) (PCL). The spiral structure with open geometries, large surface areas, and porosity will be helpful for improving nutrient transport and cell penetration into the scaffolds, which are otherwise limited in conventional tissue-engineered scaffolds for large bone defects repair. To investigate the effect of structure and fiber coating on the performance of the scaffolds, three groups of scaffolds including cylindrical PCL scaffolds, spiral PCL scaffolds (without fiber coating), and spiral-structured fibrous PCL scaffolds (with fiber coating) have been prepared. The morphology, porosity, and mechanical properties of the scaffolds have been characterized. Furthermore, human osteoblast cells are seeded on these scaffolds, and the cell attachment, proliferation, differentiation, and mineralized matrix deposition on the scaffolds are evaluated. The results indicated that the spiral scaffolds possess porosities within the range of human trabecular bone and an appropriate pore structure for cell growth, and significantly lower compressive modulus and strength than cylindrical scaffolds. When compared with the cylindrical scaffolds, the spiral-structured scaffolds demonstrated enhanced cell proliferation, differentiation, and mineralization and allowed better cellular growth and penetration. The incorporation of nanofibers onto spiral scaffolds further enhanced cell attachment, proliferation, and differentiation. These studies suggest that spiral-structured nanofibrous scaffolds may serve as promising alternatives for bone tissue engineering applications. SN - 1552-4965 UR - https://www.unboundmedicine.com/medline/citation/19642211/Spiral_structured_nanofibrous_3D_scaffolds_for_bone_tissue_engineering_ L2 - https://doi.org/10.1002/jbm.a.32591 DB - PRIME DP - Unbound Medicine ER -