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Investigation of toxin genes by polymerase chain reaction in Staphylococcus aureus strains isolated from bovine mastitis in Turkey.
Foodborne Pathog Dis. 2009 Oct; 6(8):1029-35.FP

Abstract

Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs), and toxic shock syndrome toxin-1. The aim of the present study was to characterize coa-positive S. aureus strains (n = 92) isolated from bovine subclinical mastitis in Turkey by polymerase chain reaction (PCR) amplification of exfoliative toxin (eta and etb) and toxic shock syndrome toxin-1 (tsst) genes. In addition, a multiplex PCR was employed to investigate the presence of SE genes sea, seb, sec, sed, see, seg, seh, sej, and sei. By PCR amplification, while eta and etb were not detected, only three isolates (3.3%) were positive for tsst. Twenty-seven (29.3%) isolates harbored one or more SE genes, and sei was the most common pattern by multiplex PCR. None of the isolates harbored the genes encoding sea, see, and seh. The application of this multiplex PCR assay could enable more samples to be rapidly characterized for enterotoxin production of S. aureus isolates from milk for epidemiological studies.

Authors+Show Affiliations

Department of Microbiology, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey. mkarahan@firat.edu.trNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19642915

Citation

Karahan, Murat, et al. "Investigation of Toxin Genes By Polymerase Chain Reaction in Staphylococcus Aureus Strains Isolated From Bovine Mastitis in Turkey." Foodborne Pathogens and Disease, vol. 6, no. 8, 2009, pp. 1029-35.
Karahan M, Açik MN, Cetinkaya B. Investigation of toxin genes by polymerase chain reaction in Staphylococcus aureus strains isolated from bovine mastitis in Turkey. Foodborne Pathog Dis. 2009;6(8):1029-35.
Karahan, M., Açik, M. N., & Cetinkaya, B. (2009). Investigation of toxin genes by polymerase chain reaction in Staphylococcus aureus strains isolated from bovine mastitis in Turkey. Foodborne Pathogens and Disease, 6(8), 1029-35. https://doi.org/10.1089/fpd.2009.0304
Karahan M, Açik MN, Cetinkaya B. Investigation of Toxin Genes By Polymerase Chain Reaction in Staphylococcus Aureus Strains Isolated From Bovine Mastitis in Turkey. Foodborne Pathog Dis. 2009;6(8):1029-35. PubMed PMID: 19642915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of toxin genes by polymerase chain reaction in Staphylococcus aureus strains isolated from bovine mastitis in Turkey. AU - Karahan,Murat, AU - Açik,Mehmet Nuri, AU - Cetinkaya,Burhan, PY - 2009/8/1/entrez PY - 2009/8/1/pubmed PY - 2010/1/16/medline SP - 1029 EP - 35 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 6 IS - 8 N2 - Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs), and toxic shock syndrome toxin-1. The aim of the present study was to characterize coa-positive S. aureus strains (n = 92) isolated from bovine subclinical mastitis in Turkey by polymerase chain reaction (PCR) amplification of exfoliative toxin (eta and etb) and toxic shock syndrome toxin-1 (tsst) genes. In addition, a multiplex PCR was employed to investigate the presence of SE genes sea, seb, sec, sed, see, seg, seh, sej, and sei. By PCR amplification, while eta and etb were not detected, only three isolates (3.3%) were positive for tsst. Twenty-seven (29.3%) isolates harbored one or more SE genes, and sei was the most common pattern by multiplex PCR. None of the isolates harbored the genes encoding sea, see, and seh. The application of this multiplex PCR assay could enable more samples to be rapidly characterized for enterotoxin production of S. aureus isolates from milk for epidemiological studies. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/19642915/Investigation_of_toxin_genes_by_polymerase_chain_reaction_in_Staphylococcus_aureus_strains_isolated_from_bovine_mastitis_in_Turkey_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2009.0304?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -