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Bioassay guided purification of the antimicrobial fraction of a Brazilian propolis from Bahia state.
BMC Complement Altern Med. 2009 Jul 30; 9:25.BC

Abstract

BACKGROUND

Brazilian propolis type 6 (Atlantic forest, Bahia) is distinct from the other types of propolis especially due to absence of flavonoids and presence of other non-polar, long chain compounds, but presenting good in vitro and in vivo antimicrobial activity. Several authors have suggested that fatty acids found in this propolis might be responsible for its antimicrobial activity; however, so far no evidence concerning this finding has been reported in the literature. The goals of this study were to evaluate the antibacterial activity of the main pure fatty acids in the ethanolic extract and fractions and elucidate the chemical nature of the bioactive compounds isolated from Brazilian propolis type 6.

METHODS

Brazilian propolis type 6 ethanolic extract (EEP), hexane fraction (H-Fr), major fatty acids, and isolated sub-fractions were analyzed using high performance liquid chromatography (HPLC), high resolution gas chromatography with flame ionization detection (HRGC-FID), and gas chromatography-mass spectrometry (GC-MS). Three sub-fractions of H-Fr were obtained through preparative HPLC. Antimicrobial activity of EEP, H-Fr, sub-fractions, and fatty acids were tested against Staphyloccus aureus ATCC 25923 and Streptococcus mutans Ingbritt 1600 using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).

RESULTS

EEP and H-Fr inhibited the growth of the microorganisms tested; nevertheless, no antimicrobial activity was found for the major fatty acids. The three sub-fractions (1, 2, and 3) were isolated from H-Fr by preparative HPLC and only sub-fraction 1 showed antimicrobial activity.

CONCLUSION

a) The major fatty acids tested were not responsible for the antimicrobial activity of propolis type 6; b) Sub-fraction 1, belonging to the benzophenone class, was responsible for the antimicrobial activity observed in the present study. The identification of the bioactive compound will improve the development of more efficient uses of this natural product.

Authors+Show Affiliations

Department of Physiological Sciences, Piracicaba Dental School, University of Campinas; Avenida Limeira, Piracicaba, SP 13414-903, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19643008

Citation

Castro, Myrella Lessio, et al. "Bioassay Guided Purification of the Antimicrobial Fraction of a Brazilian Propolis From Bahia State." BMC Complementary and Alternative Medicine, vol. 9, 2009, p. 25.
Castro ML, Vilela WR, Zauli RC, et al. Bioassay guided purification of the antimicrobial fraction of a Brazilian propolis from Bahia state. BMC Complement Altern Med. 2009;9:25.
Castro, M. L., Vilela, W. R., Zauli, R. C., Ikegaki, M., Rehder, V. L., Foglio, M. A., de Alencar, S. M., & Rosalen, P. L. (2009). Bioassay guided purification of the antimicrobial fraction of a Brazilian propolis from Bahia state. BMC Complementary and Alternative Medicine, 9, 25. https://doi.org/10.1186/1472-6882-9-25
Castro ML, et al. Bioassay Guided Purification of the Antimicrobial Fraction of a Brazilian Propolis From Bahia State. BMC Complement Altern Med. 2009 Jul 30;9:25. PubMed PMID: 19643008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioassay guided purification of the antimicrobial fraction of a Brazilian propolis from Bahia state. AU - Castro,Myrella Lessio, AU - Vilela,Walquíria Ribeiro, AU - Zauli,Rogéria Cristina, AU - Ikegaki,Masaharu, AU - Rehder,Vera Lúcia Garcia, AU - Foglio,Mary Ann, AU - de Alencar,Severino Matias, AU - Rosalen,Pedro Luiz, Y1 - 2009/07/30/ PY - 2009/03/19/received PY - 2009/07/30/accepted PY - 2009/8/1/entrez PY - 2009/8/1/pubmed PY - 2009/10/20/medline SP - 25 EP - 25 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 9 N2 - BACKGROUND: Brazilian propolis type 6 (Atlantic forest, Bahia) is distinct from the other types of propolis especially due to absence of flavonoids and presence of other non-polar, long chain compounds, but presenting good in vitro and in vivo antimicrobial activity. Several authors have suggested that fatty acids found in this propolis might be responsible for its antimicrobial activity; however, so far no evidence concerning this finding has been reported in the literature. The goals of this study were to evaluate the antibacterial activity of the main pure fatty acids in the ethanolic extract and fractions and elucidate the chemical nature of the bioactive compounds isolated from Brazilian propolis type 6. METHODS: Brazilian propolis type 6 ethanolic extract (EEP), hexane fraction (H-Fr), major fatty acids, and isolated sub-fractions were analyzed using high performance liquid chromatography (HPLC), high resolution gas chromatography with flame ionization detection (HRGC-FID), and gas chromatography-mass spectrometry (GC-MS). Three sub-fractions of H-Fr were obtained through preparative HPLC. Antimicrobial activity of EEP, H-Fr, sub-fractions, and fatty acids were tested against Staphyloccus aureus ATCC 25923 and Streptococcus mutans Ingbritt 1600 using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). RESULTS: EEP and H-Fr inhibited the growth of the microorganisms tested; nevertheless, no antimicrobial activity was found for the major fatty acids. The three sub-fractions (1, 2, and 3) were isolated from H-Fr by preparative HPLC and only sub-fraction 1 showed antimicrobial activity. CONCLUSION: a) The major fatty acids tested were not responsible for the antimicrobial activity of propolis type 6; b) Sub-fraction 1, belonging to the benzophenone class, was responsible for the antimicrobial activity observed in the present study. The identification of the bioactive compound will improve the development of more efficient uses of this natural product. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/19643008/Bioassay_guided_purification_of_the_antimicrobial_fraction_of_a_Brazilian_propolis_from_Bahia_state_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-9-25 DB - PRIME DP - Unbound Medicine ER -